1887

Abstract

This study outlines the effects of a modification of the actin-based cytoskeleton on the maturation of rabies virus in human neuroblastoma cell and primary rat cortical neuron cultures. In a Ca-depleted or an EGTA-containing medium, disruption of microfilaments did not affect intracellular viral nucleoprotein synthesis, as demonstrated by dual-immunofluorescence microscopy, and caused no change in the extracellular titre of rabies virus. Furthermore, the continuous presence of the anti-calmodulin drugs trifluoperazine (1 to 20 µ) and chlorpromazine (1 to 30 µ), or the -type Ca channel antagonist nifedepine (1 to 10 µ) or the Ca-specific ionophore A23187 (0.05 to 1.0 µ), did not modify the extracellular titre of rabies virus significantly over a 48 h period. The inference from these studies is that the maturation of rabies virus is independent of the integrity of the microfilament structures and calmodulin-dependent processes of neuronal cells.

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1991-09-01
2021-10-19
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References

  1. Birtch M., Allen T. D. 1980; The modulation of cellular contractility and adhesion by trypsin and EGTA. Experimental Cell Research 125:221–231
    [Google Scholar]
  2. Bohn W., Rutter G., Hohenberg H., Mannweiler K. 1983; Inhibition of measles virus budding by phenothiazines. Virology 130:44–55
    [Google Scholar]
  3. Bohn W., Rutter G., Hohenberg H., Mannweiler K., Nobis P. 1986; Involvement of actin filaments in budding of measles virus: studies on cytoskeletons of infected cells. Virology 149:91–106
    [Google Scholar]
  4. Bussereau F., Perrin P. 1982; Cellular response to rabies virus infection. Comparative Immunology and Microbiology of Infectious Diseases 5:49–59
    [Google Scholar]
  5. Damsky C. H., Sheffield J. B., Tuszynski G. P., Warren L. 1977; Is there a role for actin in viral budding?. Journal of Cell Biology 75:595–605
    [Google Scholar]
  6. Dix R. D., Courtney R. J. 1976; Effects of cytochalasin B on herpes simplex virus type I replication. Virology 70:127–135
    [Google Scholar]
  7. Genty N., Bussereau F. 1980; Is cytoskeleton involved in VSV reproduction?. Journal of Virology 34:777–781
    [Google Scholar]
  8. Griffin J. A., Compans R. W. 1979; Effect of cytochalasin B on the maturation of enveloped viruses. Journal of Experimental Medicine 150:379–391
    [Google Scholar]
  9. Heggeness M. H., Scheid A., Choppin P. W. 1980; Conformation of the helical nucleocapsids of paramyxoviruses and vesicular stomatitis Virus: reversible coiling and uncoiling induced by changes in salt concentration. Proceedings of the National Academy of Sciences, U.S.A. 77:2631–2635
    [Google Scholar]
  10. Iwasaki Y., Minamoto N. 1982; Scanning and freeze-fracture electron microscopy of rabies virus infection in murine neuroblastoma cells. Comparative Immunology and Microbiology of Infectious Diseases 5:1–8
    [Google Scholar]
  11. Iwasaki Y., Liu D.-S., Yamamoto T., Konno H. 1985; On the replication and spread of rabies virus in the human central nervous system. Journal of Neuropathology and Experimental Neurology 44:185–195
    [Google Scholar]
  12. Jones K. H., Senft J. A. 1985; An improved method to determine cell viability by simultaneous staining with fluorescein diacetate - propidium iodide. Journal of Histochemistry and Cytochemistry 33:77–79
    [Google Scholar]
  13. Lockhart B. P., Tsiang H., Ceccaldi P. E., Guillemer S. 1991; Ketamine-mediated inhibition of rabies virus infection in vitro and in rat brain. Antiviral Chemistry and Chemotherapy 2:9–15
    [Google Scholar]
  14. Lycke E., Tsiang H. 1987; Rabies virus infection of cultured rat sensory neurones. Journal of Virology 61:2733–2741
    [Google Scholar]
  15. Metsikko K., Simons K. 1986; The budding mechanism of spikeless vesicular stomatitis virus particles. EMBO Journal 5:1913–1920
    [Google Scholar]
  16. Moyer S. A., Baker S. C., Horikami S. M. 1990; Host cell proteins required for measles virus reproduction. Journal of General Virology 71:775–783
    [Google Scholar]
  17. Naito S., Matsumoto S. 1978; Identification of cellular actin with rabies virus. Virology 91:151–163
    [Google Scholar]
  18. Odenwald W. F., Arnheiter H., Dubois-Dalcq M., Lazzarini R. A. 1986; Stereo images of vesicular stomatitis virus assembly. Journal of Virology 57:922–932
    [Google Scholar]
  19. Ono K., Dubois-Dalcq M., Schubert M., Lazzarini R. A. 1987; A mutated membrane protein of vesicular stomatitis virus has an abnormal distribution within the infected cell and causes defective budding. Journal of Virology 61:1332–1341
    [Google Scholar]
  20. Osborn M., Weber K. 1980a; Dimethylsulphoxide and the ionophore A23187 affect the arrangement of actin and induce nuclear actin paracrystals in PtK2 cells. Experimental Cell Research 129:103–114
    [Google Scholar]
  21. Osborn M., Weber K. 1980b; Damage of cellular functions by trifluoperazine, a calmodulin-specific drug. Experimental Cell Research 130:484–488
    [Google Scholar]
  22. Pal R., Wagner R. R. 1987; Rhabdovirus membrane and maturation. In The Rhaboviruses pp 75–128 Edited by Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  23. Piazza G. A., Wallace R. W. 1985; Calmodulin accelerates the rate of polymerization of human platelet actin and alters the structural characteristics of actin filaments. Proceedings of the National Academy of Sciences, U.S.A 82:1683–1687
    [Google Scholar]
  24. Simons K., Garoff H. 1980; The budding mechanisms of enveloped animal viruses. Journal of General Virology 50:1–21
    [Google Scholar]
  25. Smith A. L., Tignor G. H., Mifune K., Motohashi T. 1977; Isolation and assay of rabies serogroup viruses in CER cells. Intervirology 8:92–99
    [Google Scholar]
  26. Trudeau D. L., Freier E. F. 1967; Determination of Ca2+ in urine and serum by atomic absorption spectrophotometry (ASS). Clinical Chemistry 13:101–114
    [Google Scholar]
  27. Wang E., Wolf B. A., Lamb R. A., Choppin P. W., Goldberg A. R. 1976; The presence of actin in enveloped virus. In Cell Motility pp 589–600 Edited by Goldberg R., Pollard T., Rosenbaum J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
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