1887

Abstract

The sequence of the 3′-terminal 1865 nucleotides of the genome of the tobamovirus odontoglossum ringspot virus (ORSV) was determined. This sequence contained two open reading frames (ORFs), 912 and 477 nucleotides long. The 912 nucleotide ORF has been identified as the cell-to-cell transport protein gene. The 477 nucleotide ORF was expressed in , and the product was detected by antibodies specific for the coat protein of ORSV. The amino acid sequence of protein encoded by this ORF shares 84% similarity with the tobacco mosaic virus (TMV) (vulgare) coat protein. The 3′-terminal untranslated region of ORSV comprises 414 nucleotides, 210 nucleotides more than that of TMV (vulgare) RNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-9-2247
1991-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/9/JV0720092247.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-9-2247&mimeType=html&fmt=ahah

References

  1. Corbett M. K. 1967; Some distinguishing characteristics of the orchid strain of tobacco mosaic virus. Phytopathology 57:164–172
    [Google Scholar]
  2. Haseloff J., Symons R. H. 1981; Chrysanthemum stunt viroid: primary sequence and secondary structure. Nucleic Acids Research 9:2741–2752
    [Google Scholar]
  3. Ikegami M., Osaki T., Inouye T. 1987; Single-stranded DNA in tobacco leaf curl virus. Annals of the Phytopathological Society of Japan 53:269–273
    [Google Scholar]
  4. Inouye N. 1966; A virus disease of Cymbidium caused by odontoglossum ring spot virus in Japan. Berichte des Ohara Instituts für Landwirtschaftliche Biologie Bd. XIII, Heft 3: pp 149–159
    [Google Scholar]
  5. Isomura Y., Matumoto Y., Murayama A., Chatani M., Inouye N., Ikegami M. 1990; Nucleotide sequence of cell-to-cell transport protein gene of odontoglossum ringspot virus. Nucleic Acids Research 18:7448
    [Google Scholar]
  6. Jensen D. D., Gold H. A. 1951; A virus ring spot of Odontoglossum orchid: symptoms, transmission and electron microscopy. Phytopathology 41:648–653
    [Google Scholar]
  7. Kado C. I., van Regenmortel M. H. V., Knight C. A. 1968; Studies on some strains of tobacco mosaic virus in orchids. I. Biological, chemical and serological studies. Virology 34:17–24
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Lawson R. H. 1970; Etiology of flower necrosis in Cattleya orchid. Phytopathology 60:36–40
    [Google Scholar]
  10. Murayama A., Yamaya Y., Ikegami M. 1991; Identification of the coat protein gene of bean golden mosaic geminivirus. Intervirology 32:258–261
    [Google Scholar]
  11. Paul H. L. 1975; Odontoglossum ringspot virus. CMI/AAB Descriptions of Plant Viruses no 155
    [Google Scholar]
  12. Sippel A. E. 1973; Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli. European Journal of Biochemistry 37:31–40
    [Google Scholar]
  13. Van Belkum A., Abrahams J. P., Pleij C. W. A., Bosch L. 1985; Five pseudoknots are present at the 204 nucleotides long 3′ non-coding region of tobacco mosaic virus. Nucleic Acids Research 13:7673–7678
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-9-2247
Loading
/content/journal/jgv/10.1099/0022-1317-72-9-2247
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error