Influence of the C terminus of the small protein subunit of bean pod mottle virus on the antigenicity of the virus determined using monoclonal antibodies and anti-peptide antiserum
Middle component particles of bean pod mottle virus (BPMV) containing small protein subunits with a cleaved C terminus were used to produce monoclonal antibodies (MAbs). All MAbs were specific for cryptotopes, i.e. epitopes present only on dissociated BPMV protein. The MAbs reacted more strongly with virus protein preparations containing the cleaved form of the small subunit than with preparations containing only the uncleaved form. It seems that the presence of additional residues at the C terminus of the intact small subunit interferes with antibody binding. Antibodies raised against synthetic peptides corresponding to the C terminus of the uncleaved small subunit reacted with both intact virions and dissociated subunits. This C-terminal region seems to play a dominant role in the antigenicity of the virus.
BaranyG.,
MerrifieldR. B.1980; Solid-phase peptide synthesis. In The Peptides. Analysis, Synthesis, Biology vol 2 Special Methods in Peptide Synthesis pp 3–285 Edited by
GrossE.,
MeienhoferJ.
New York: Academic Press;
BriandJ. P.,
MullerS.,
Van RegenmortelM. H. V.1985; Synthetic peptides as antigens: pitfalls of conjugation methods. Journal of Immunological Methods 78:59–69
DekkerE. L.,
PortaC.,
Van RegenmortelM. H. V.1989; Limitations of different ELISA procedures for localizing epitopes in viral coat protein subunits. Archives of Virology 105:269–286
DoreI.,
WeissE.,
AltschuhD.,
Van RegenmortelM. H. V.1988; Visualization by electron microscopy of the location of tobacco mosaic virus epitopes reacting with monoclonal antibodies in enzyme immunoassay. Virology 162:279–289
HardieG.,
Van RegenmortelM. H. V.1977; Isolation of specific antibody under conditions of low ionic strength. Journal of Immunological Methods 15:305–314
HuguenotC.,
GivordL.,
SommermeyerG.,
Van RegenmortelM. H. V.1989; Differentiation of peanut clump virus serotypes by monoclonal antibodies. Research in Virology 140:87–102
JeagléM.,
Van RegenmortelM. H. V.1985; Use of ELISA for measuring the extent of serological cross-reactivity between plant viruses. Journal of Virological Methods 11:189–198
JaegléM.,
BriandJ. P.,
BurckardJ.,
Van RegenmortelM. H. V.1988; Accessibility of three continuous epitopes in tomato bushy stunt virus. Annales de l’Institut Pasteur Virologie 139:39–50
KalmarG. B.,
EastwellK. C.1989a; Reaction of coat proteins of two comoviruses in different aggregation states with monoclonal antibodies. Journal of General Virology 70:3451–3457
KalmarG. B.,
EastwellK. C.1989b; Serological differentiation between top component and nucleoprotein components of comoviruses. Journal of General Virology 70:3459–3464
MackenzieD. J.,
TremaineJ. H.1986; The use of a monoclonal antibody specific for the N-terminal region of southern bean mosaic virus as a probe of virus structure. Journal of General Virology 67:727–735
PolsonA.,
von WechmarM. B.,
Van RegenmortelM. H. V.1980; Isolation of viral IgY antibodies from yolks of immunized hens. Immunological Communications 9:475–493
ShepherdR. J.1963; Serological relationship between bean pod mottle virus and cowpea mosaic viruses from Arkansas and Trinidad. Phytopathology 53:865–866
StockerJ. W.,
FosterH. K.,
MiggianoV.,
StahliC.,
StaigerG.,
TakacsB.,
StaehelinT.1982; Generation of two new myeloma cell lines ‘PAT and ‘PAI-O’ for hybridoma production. Research Disclosures 217:155–157
SwaansH.,
Van KammenA.1973; Reconsideration of the distinction between the severe and yellow strains of cowpea mosaic virus. Netherlands Journal of Plant Pathology 79:257–265
TremaineJ. H.,
RonaldW. P.,
MackenzieD. J.1985b; Southern bean mosaic virus monoclonal antibodies: reactivity with virus strains and with the virus antigen in different conformations. Phytopathology 75:1208–1212
Van RegenmortelM. H. V.1986; The potential for using monoclonal antibodies in the detection of plant viruses. In Developments and Applications in Virus Testing pp 89–101 Edited by
JonesR. A. C.,
TorranceL.
Wellesbourne: Association of Applied Biologists;
WesthofE.,
AltschuhA.,
MorasD.,
BloomerA. C.,
MondragonA.,
Van RegenmortelM. H. V.1984; Correlation between segmental mobility and the location of antigenic determinants in proteins. Nature, London 311:123–126
ZreinM.,
BurckardJ.,
Van RegenmortelM. H. V.1986; Use of the biotin–avidin system for detecting a broad range of serologically related plant viruses by ELISA. Journal of Virological Methods 13:121–128
Influence of the C terminus of the small protein subunit of bean pod mottle virus on the antigenicity of the virus determined using monoclonal antibodies and anti-peptide antiserum