RT Journal Article SR Electronic(1) A1 de Haan, Peter A1 Kormelink, Richard A1 de Oliveira Resende, Renato A1 van Poelwijk, Frank A1 Peters, Dick A1 Goldbach, RobYR 1991 T1 Tomato spotted wilt virus L RNA encodes a putative RNA polymerase JF Journal of General Virology, VO 72 IS 9 SP 2207 OP 2216 DO https://doi.org/10.1099/0022-1317-72-9-2207 PB Microbiology Society, SN 1465-2099, AB The complete nucleotide sequence of the large (L) genome segment of tomato spotted wilt virus (TSWV) has been determined. The RNA is 8897 nucleotides long and contains complementary 3′ and 5′ ends, comprising 62 nucleotides at the 5′ end and 66 nucleotides at the 3′ end. The RNA is of negative polarity, with one large open reading frame (ORF) located on the viral complementary strand. This ORF corresponds to a primary translation product of 2875 amino acids in length, with a predicted M r of 331500. Comparison with the polymerase proteins of other negative-strand viruses indicates that this protein most likely represents the viral polymerase. The genetic organization of TSWV L RNA is similar to that of the L RNA segments of Bunyamwera and Hantaan viruses, animal-infecting representatives of the Bunyaviridae., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-9-2207