1887

Abstract

Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5′ ORF) and 822 (3′ ORF) bases. The 3′ ORF is virtually identical in sequence to RNA-3, which encodes the 30509 (30K) coat protein. The 5′ ORF encodes an 38860 (39K) protein which slightly resembles the 32K protein encoded by RNA-3 of alfalfa mosaic virus (AIMV). RBDV RNA-2 resembles AIMV RNA-3 in being bicistronic and encoding a coat protein at the 3′ end. Comparing RNA-1 and RNA-2 of RBDV, only the 18 3′-terminal nucleotides are identical in sequence but the 3′-terminal 71 nucleotides of each RNA species have the potential to form similar stem-loop structures.

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/content/journal/jgv/10.1099/0022-1317-72-9-2183
1991-09-01
2019-11-18
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-9-2183
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