A comparison of WIN 51711 and R 78206 as stabilizers of poliovirus virions and procapsids Free

Abstract

The thermal denaturation of poliovirus virions and procapsids in the 42 to 48 °C range was studied using N- and H-specific monoclonal antibodies. The half-life of the N antigen of Mahoney and Sabin 1 virions was extended 50- to 250-fold by either 10 µ WIN 51711 or R 78206. The minimum concentrations required for full stabilization at 46 °C (1.0 µ for WIN 51711, 0.2 µ for R 78206) were independent of the strain or serotype of the virus; 30 to 60 molecules of stabilizer per virion were required for full protection. R 78206 was the most efficient stabilizer of Mahoney procapsids; the half-life of the N-specific epitopes of these particles at 44 °C was extended from less than 1 min to 1 day.

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1991-09-01
2024-03-29
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References

  1. Andries K., Dewindt B., De Brabander M., Stokbroekx R., Janssen P. A. J. 1988; In vitro activity of R61837, a new antirhinovirus compound. Archives of Virology 101:155–167
    [Google Scholar]
  2. Andries K., Dewindt B., Snoeks J., Willebrords R. 1989; Lack of quantitative correlation between inhibition of replication of rhinoviruses by an antiviral drug and their stabilization. Archives of Virology 106:51–61
    [Google Scholar]
  3. Andries K., Dewindt B., Snoeks J., Wouters L., Moereels H., Lewi P. J., Janssen P. A. J. 1990; Two groups of rhinoviruses revealed by a panel of antiviral compounds present sequence divergence and differential pathogenicity. Journal of Virology 64:1117–1123
    [Google Scholar]
  4. Bauer D. J., Selway J. W. T., Batchelor J. F., Tisdale M., Caldwell J. C., Young D. A. B. 1981; 4′, 6-dichloroflavan (BW683C), a new anti-rhinovirus compound. Nature, London 292:369–370
    [Google Scholar]
  5. Brioen P., Sijens R. J., Vrijsen R., Rombaut B., Thomas A. A. M., Jackers A., Boeye A. 1982; Hybridoma antibodies to poliovirus N and H antigen. Archives of Virology 74:325–330
    [Google Scholar]
  6. Chapman M. S., Minor I., Rossmann M. G., Diana G. D., Andries K. 1991; Human rhinovirus 14 complexed with antiviral compound R 61837. Journal of Molecular Biology 217:455–463
    [Google Scholar]
  7. Everaert L., Vrijsen R., Boeye A. 1989; Eclipse products of poliovirus in cold-synchronized HeLa cells. Virology 171:76–82
    [Google Scholar]
  8. Ishitsuka H., Ninomya Y. N., Ohsawa C., Fujiu M., Suhura Y. 1982; Direct and specific inactivation of rhinovirus by chalcone Ro 09-410. Antimicrobial Agents and Chemotherapy 22:617–621
    [Google Scholar]
  9. Kenny M. T., Dulworth J. K., Bargar T. M., Torney H. L., Graham M. C., Manelli A. M. 1986; In vitro antiviral activity of the 6-substituted 2-(3′, 4′-dichlorophenoxy)-2H-pyrano[2, 3-b]pyridines MDL 20, 610, MDL 20, 646, and MDL 20, 957. Antimicrobial Agents and Chemotherapy 30:516–518
    [Google Scholar]
  10. Kohara M., Omata T., Kameda A., Semler B. L., Itoh H., Wimmer E., Nomoto A. 1985; In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin-1 strain. Journal of Virology 53:786–792
    [Google Scholar]
  11. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virologyv 67:1283–1291
    [Google Scholar]
  12. Nakano J. H., Hatch M. H., Thieme M. L., Nottay B. 1978; Parameters for differentiating vaccine-derived and wild poliovirus strains. Progress in Medical Virology 2:178–206
    [Google Scholar]
  13. Otto M. J., Fox M. P., Fancher M. J., Kuhrt M. F., Diana G. D., McKinlay M. A. 1985; In vitro activity of WIN 51711, a new broad-spectrum antipicornavirus drug. Antimicrobial Agents and Chemotherapy 27:883–886
    [Google Scholar]
  14. Rombaut B., Boeye A. 1991; In vitro assembly of poliovirus 14S subunits: WIN 51711 stabilization as a model for the antigenicity conferring activity of infected cell extracts. Virology 180:788–792
    [Google Scholar]
  15. Rombaut B., Vrijsen R., Boeye A. 1985; Stabilization by host cell components and Mg2+ of the neutralization epitopes of poliovirus. Journal of General Virology 66:303–307
    [Google Scholar]
  16. Rombaut B., Boeye A., Ferguson M., Minor P. D., Mosser A., Rueckert R. 1990a; Creation of an antigenic site in poliovirus type 1 by assembly of 14S subunits. Virology 174:305–307
    [Google Scholar]
  17. Rombaut B., Brioen P., Boeye A. 1990b; Disoxaril stabilization and immunogenicity of poliovirus procapsids. Journal of General Virology 71:1081–1086
    [Google Scholar]
  18. Rombaut B., Foriers A., Boeye A. 1990c; Purification of poliovirus 14S subunits by sucrose gradient ultracentrifugation and high-performance size-exclusion chromatography. Journal of Virological Methods 29:303–312
    [Google Scholar]
  19. Smith T. J., Kremer M. J., Luo M., Vriend G., Arnold E., Kamer G., Rossmann M. G., McKinlay M. A., Diana G. D., Otto M. J. 1986; The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating. Science 233:1286–1293
    [Google Scholar]
  20. Vrijsen R., Rombaut B., Boeye A. 1983; A simple quantitative protein A micro-immunoprecipitation method; assay of antibodies to the N and H antigens of poliovirus. Journal of Immunological Methods 59:217–220
    [Google Scholar]
  21. Wallis C., Melnick J. L. 1961; Stabilization of poliovirus by cations. Texas Reports in Biology and Medicine 19:683–700
    [Google Scholar]
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