Antigenic variation of European haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies Free

Abstract

Monocloral antibodies (MAbs) against Puumala (PUU) virus, the aetiological agent of nephropathia epidemica, were produced by fusing activated spleen cells from a bank vole () with the mouse myeloma cell line SP2/0. This novel approach, utilizing the natural vector of PUU virus for hybridoma production, proved to be highly efficient, and eight stable PUU virus-specific heterohybridomas were isolated and characterized. The bank vole MAbs were all specific for the nucleocapsid protein (N) of PUU virus, as determined by immunoprecipitation. When evaluated by additivity immunoassays, the MAbs were found to recognize several different, distinct or overlapping, epitopes on N. The MAbs were used in immunofluorescence assays to compare eight PUU-related virus isolates, and the prototype Hantaan, Urban rat and Prospect Hill viruses. The reactivity varied among the different MAbs and could be classified into five groups. One MAb reacted exclusively with PUU-related viruses; two MAbs reacted with all PUU-related virus strains tested, as well as Prospect Hill virus, but did not react with Urban rat virus and Hantaan virus; one MAb reacted with all PUU-related virus strains tested and weakly with Hantaan virus, but not with Urban rat and Prospect Hill viruses; two MAbs reacted with all the virus strains tested. Two virus strains, K-27 and CG-1820, isolated in the western U.S.S.R., were distinguished from the other PUU-related virus strains by two MAbs, suggesting that the large group of independently isolated PUU-related viruses may be more heterogeneous than previously believed.

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1991-09-01
2024-03-28
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References

  1. Arikawa J., Schmaljohn A. L., Dalrymple J. M., Schmaljohn C. S. 1989; Characterization of Hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies. Journal of General Virology 70:615–624.
    [Google Scholar]
  2. Baek L. J., Yanagihara R., Gibbs C. J. Jr, Miyazaki M, Gajdusek D. C. 1988; Leakey virus: a new hantavirus isolated from Mus musculus in the United States.. Journal of General Virology 69:3129–3132
    [Google Scholar]
  3. Bogdanova S. B., Gavrilovskaya I. N., Boyko V. A., Prokhorova N. A., Linev M. B., Apekina N. S., Gorbachkova Ye. A., Rymalov Nl. V., Bernshteyn A. D., Chumakov M. P. 1987; Persistent infection caused by hemorrhagic fever with renal syndrome in red mice (Clethrionomys glareolus), natural hosts of the virus.. Mikrobiologicheskiv zhurnal 49:99–106.
    [Google Scholar]
  4. Brummer-Korvenkontio M., Vaheri A., Hovi T., von Bonsdorff C. H., Vuorimies J., Manni T., Penttinen K., Oker-Blom N & Lä, Hdevirta J. 1980; Nephropathia epidemica. Detection of antigen in bank voles and serological diagnosis of human infection.. Journal of Infectious Diseases 141:131–134.
    [Google Scholar]
  5. Franko M. C., Gibbs C. J., Lee P. W., Gajdusek D. C. 1983; Monoclonal antibodies specific for Hantaan virus. Proceedings of the National Academy of Sciences, U.S. A. 80:4149–4153
    [Google Scholar]
  6. Friguet B., Djavadi-Ohaniance L., Pages J., Bussard A., Goldberg M. 1983; A convenient enzyme-linked immunosorbent assay for testing whether monoclonal antibodies recognize the same antigenic site. Application to hybridomas specific for the β2-subunit of Escherichia coli tryptophan synthetase.. Journal of Immunological Methods 60:351–358
    [Google Scholar]
  7. Goding J. W. 1980; Antibody production of hybridomas. Journal of Immunological Methods 39:285–308
    [Google Scholar]
  8. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Lähdevirta J. 1971; Nephropathia epidemica in Finland. A clinical, histological and epidemiological study.. Annals of Clinical Research 3: supplement 8
    [Google Scholar]
  11. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–308
    [Google Scholar]
  12. Lee H. W., Lee P. W., Baek L. J., Song C. K., Seong I. W. 1981; Intraspecific transmission of Hantaan virus, etiologic agent of Korean hemorrhagic fever, in the rodent Apodemus agrarius. American Journal of Tropical Medicine and Hygiene 30:1106–1112
    [Google Scholar]
  13. Lee H. W., Baek L. J., Johnson K. M. 1982; Isolation of Hantaan virus, the etiologic agent of Korean haemorrhagic fever, from wild urban rats. Journal of Infectious Diseases 146:638–644
    [Google Scholar]
  14. Lee P. W., Amyx H. L., Gajdusek D. C., Yanagihara R. T., Goldgaber D., Gibbs C. J. Jr 1982; New hemorrhagic fever with renal syndrome-related virus in indigenous wild rodents in the United States. Lancet ii:1405.
    [Google Scholar]
  15. Niklasson B., LeDuc J. 1984; Isolation of the nephropathia epidemica agent in Sweden. Lancet i:1012–1013
    [Google Scholar]
  16. Niklasson B., LeDuc J. 1987; Epidemology of nephropathia epidemica in Sweden. Journal of Infectious Diseases 155:269–276
    [Google Scholar]
  17. Niklasson B., Tkachenko E., Ivanov A. P., Van der Groen G., Wiger D., Andersen H. K., LeDuc J., Kjellson T., Nystrӧm K. 1990; Hemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus specific.. Research in Virology 141:637–648.
    [Google Scholar]
  18. Schmaljohn C. S., Hasty S. E., Dalrymple J. M., LeDuc J. W., Lee H. W., Von Bonsdorff C. H., Brummer-Korvenkontio M., Vaheri A., Tsai T. F., Regnery H. L., Goldgaber D., Lee P. W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome.. Science 227:1041–1044
    [Google Scholar]
  19. Sheshberadaran H., Niklasson B., Tkachenko E.A. 1988; Antigenic relationship between hantaviruses analysed by immunoprecipitation. Journal of General Virology 69:2645–2651
    [Google Scholar]
  20. Shulman M., Wilde C. D., Köhler G. 1978; A better line for making hybridomas secreting specific antibodies. Nature, London 276:269–270
    [Google Scholar]
  21. Sugiyama K., Morikawa S., Matsuura Y., Tkachenko E. A., Morita C., Komatsu T., Akao Y., Kitamura T. 1987; Four serotypes of haemorrhagic fever with renal syndrome viruses identified by polyclonal and monoclonal antibodies. Journal of General Virology 68:979–987.
    [Google Scholar]
  22. Tkachenko E. A., Bashkirtsev V. N., Van der Groen G., Dzagurova T. K., Ivanov A. P., Ryltseva E. V. 1984; Isolation in Vero E6 cells of hantavirus from Clethrionomys glareolus captured in the Bashkiria area of the USSR. Annales de la Societe beige de medecine tropicale 65:121–135
    [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  24. Van Der Groen g. 1985; Haemorrohagic fever with renal syndrome: recent developments.. Annales de la Société beige de medecine tropicale 65:121–135
    [Google Scholar]
  25. Van Der Groen G., Beelaert G., Hoofd G., Leirs H., Verhagen R., Yamanishi H., Tkachenko E. A., Ivanov A. P. 1987; Partial characterization of a hantavirus isolated from Clethrionomys glareolus captured in Belgium. Acta virologica 31:180–184
    [Google Scholar]
  26. Yamanishi K., Dantas J. R. Jr, Takahashi M., Yamanouchi T., Domae K., Takahashi Y., Tanishita O. 1984; Antigenic differences between two viruses, isolated in Japan and Korea, that cause hemorrhagic fever with renal syndrome.. Journal of Virology 52:231–237
    [Google Scholar]
  27. Yanagihara R., Gajdusek D. C. 1988; Hemorrhagic fever with renal syndrome: a historical perspective and review of recent advances. In CRC Handbook on Viral and Rickettsial Hemorrhagic Fevers. pp 151–188 Edited by Gear J. H. S. Boca Raton: CRC Press;
    [Google Scholar]
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