1887

Abstract

The mode of expression of the overlapping genes of the triple block positioned internally in potato virus X (PVX) RNA was examined. The results of translation of synthetic RNA transcripts and natural PVX-specific methylmercuric hydroxide-denatured dsRNAs suggest that the 25K protein is expressed as a single translation product of the 2.1 kb subgenomic (sg) RNA and that both the 12K and 8K proteins are expressed from the same 1.4 kb sgRNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-8-2039
1991-08-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/8/JV0720082039.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-8-2039&mimeType=html&fmt=ahah

References

  1. De Wald D. B., Adams L. D., Pearson J. D. 1986; A nonurea electrophoretic gel system for resolution of polypeptides of M r 2000 to M r 20000. Analytical Biochemistry 154:502–508
    [Google Scholar]
  2. Dolja V. V., Grama D. P., Morozov D., Yu S., Atabekov J. G. 1987; Potato virus X-related single- and double-stranded RNAs. Characterization and identification of terminal structures. FEBS Letters 214:308–312
    [Google Scholar]
  3. Dolja V. V., Karasev A. V., Agranovsky A. A. 1990; Organization of the beet yellows closterovirus genome. In New Aspects of Positive-strand RNA Viruses pp. 31–35 Edited by Rueckert R., Brinton M. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  4. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1988; A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination. FEBS Letters 235:16–24
    [Google Scholar]
  5. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  6. Jackson A. O., Hunter B. G., Gustafson G. D. 1989; Hordeivirus relationships and genome organization. Annual Review of Phytopathology 27:95–121
    [Google Scholar]
  7. Karasev A. V., Alexandrova N. M., Miroshnichenko N. A., Nesterova I. V., Dorokhov YU L. 1987; Translation activity of messenger RNAs from potato virus X-infected plants. Molekulayrnaya Biologiaya 21:1367–1377
    [Google Scholar]
  8. Karasev A. V., Miroshnichenko N. A., Ugarova T. YU. 1989; Use of Krebs-2 ascites extracts for plant virus RNA translation. Molekulyarnaya Biologiya 23:119–126
    [Google Scholar]
  9. Koenig R. 1972; Anomalous behavior of the coat proteins of potato virus X and cactus virus X during electrophoresis in dodecyl-sulfate containing polyacrylamide gels. Virology 50:263–266
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 27:680–685
    [Google Scholar]
  11. Miroshnichenko N. A., Karpova O. V., Morozov S. Yu., Rodionova N. P., Atabekov J. G. 1988; Translation arrest of potato virus X RNA in Krebs-2 cell-free system: RNase H cleavage promoted by complementary oligodeoxynucleotides. FEBS Letters 234:65–68
    [Google Scholar]
  12. Morozov S. Yu., Gorbulev V. G., Novikov V. K., Agranovsky A. A., Kozlov YU. V., Atabekov J. G., Baev A. A. 1981; The primary structure of the 5- and 3′-terminal regions of the RNA of potato virus X. Doklady Akademii Nauk SSSR 259:723–725
    [Google Scholar]
  13. Morozov S. Yu., Dolja V. V., Atabekov J. G. 1989; Probable reassortment of genomic elements among elongated RNA-containing plant viruses. Journal of Molecular Evolution 29:52–62
    [Google Scholar]
  14. Morozov S. Yu., Miroshnichenko N. A., Zelenina D. A., Fedorkin O. N., Solovyev A. G., Lukasheva L. I., Atabekov J. G. 1990; Expression of RNA transcripts of potato virus X full-length and subgenomic cDNAs. Biochimie 72:677–684
    [Google Scholar]
  15. Petty I. T. D., Jackson A. O. 1990; Mutational analysis of barley stripe mosaic virus RNA β . Virology 179:712–718
    [Google Scholar]
  16. Purcifull D. E., Edwardson J. R. 1981; Potexviruses. In Plant Virus Infections: Comparative Diagnosis pp. 627–694 Edited by Kurstak E. Amsterdam: Elsevier/North Holland:
    [Google Scholar]
  17. Robinson D. J., Mayo M. A., Fritsch C., Jones A. T., Raschke J. H. 1983; Origin and messenger activity of two small RNA species found in particles of tobacco rattle virus strain SYM. Journal of General Virology 64:1591–1599
    [Google Scholar]
  18. Rupasov V. V., Morozov S. Yu., Kanyuka K. V., Zavriev S. K. 1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. Journal of General Virology 70:1861–1869
    [Google Scholar]
  19. Skryabin K. G., Morozov S. Yu., Kraev A. S., Rozanov M. N., Chernov B. K., Lukasheva L. I., Atabekov J. G. 1988; Conserved and variable elements in RNA genomes of potexviruses. FEBS Letters 240:33–40
    [Google Scholar]
  20. Sonenberg M. N., Shatkin A. J., Ricciardi R. P., Rubin M., Goodman R. M. 1978; Analysis of terminal structures of RNA from potato virus X. Nucleic Acids Research 5:2501–2511
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-8-2039
Loading
/content/journal/jgv/10.1099/0022-1317-72-8-2039
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error