1887

Abstract

To estimate the particle size of hepatitis C virus (HCV), a major causative agent of post-transfusion non-A, non-B hepatitis, we filtered plasma or serum samples through microporous cellulose fibres with different pore sizes. The amount of HCV particles in samples before and after filtration was determined by a quantitative reverse transcriptase polymerase chain reaction (PCR) method. Since there is no quantitative biological assay for HCV, except for that in chimpanzees, the HCV titre obtained from the PCR method was used in an equation constructed previously for application to filtration experiments with a flavivirus which is distantly related to HCV. The particle was estimated to be between 30 and 38 nm in diameter, although the possibility remained that larger HCV particles or HCV aggregates with a diameter of more than 39 nm might exist. Double-step filtration through microporous cellulose fibres with a pore size of 35 nm reduced the HCV content to below levels detectable by our PCR method, indicating that it is possible to eliminate HCV particles by simple filtration techniques.

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1991-08-01
2024-12-12
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References

  1. Alter H. J., Purcell R. H., Shih J. W., Melpolder J. C., Houghton M., Choo Q.-L., Kuo G. 1989; Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. New England Journal of Medicine 321:1494–1500
    [Google Scholar]
  2. Bradley D. W., Maynard J. E., Popper H., Cook E. H., Ebert J. W., McCaustland K. A., Schable C. A., Fields H. A. 1983; Post-transfusion non-A, non-B hepatitis: physicochemical properties of two distinct agents. Journal ofInfectious Diseases 148:254–265
    [Google Scholar]
  3. Bradley D. W., McCaustland K. A., Cook E. H., Schable C. A., Ebert J. W., Maynard J. E. 1985; Post-transfusion non-A, non-B hepatitis in chimpanzees. Physicochemical evidence that the tubule-forming agent is a small, enveloped virus. Gastroenterology 88:773–779
    [Google Scholar]
  4. Chomoczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidine thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  5. Choo Q.-L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 241:359362
    [Google Scholar]
  6. Choo Q. L., Richman K., Han J., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby A., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; The genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, U,. S,. A 88:2451–2455
    [Google Scholar]
  7. Esteban J. I., Esteban R., Viladomiu L., LÓ-Talavera J. C., Gonzalez A., Hernandez J. M., Roget M., Vargas V., Genesca J., Buti M., Guardia J., Houghton M., Choo Q.-L., Kuo G. 1989; Hepatitis C virus antibodies among risk groups in Spain. Lancet ii:294–296
    [Google Scholar]
  8. Feinstone S. M., Mihalik K. B., Kamimura T., Alter H. J., London W. T., Purcell R. H. 1983; Inactivation of hepatitis B virus and non-A, non-B hepatitis by chloroform. Infection and Immunity 41:816–821
    [Google Scholar]
  9. Hamamoto Y., Harada S., Kobayashi S., Yamaguchi K., Iijima H., Manabe S., Tsurumi T., Aizawa H., Yamamoto N. 1989; A novel method for removal of human immunodeficiency virus (HIV): filtration with porous polymeric membranes. Vox sanguinis 56:230–235
    [Google Scholar]
  10. Han J. H., Shyamala V., Richman K. H., Brauer M. J., Tekamp-Olson P., Irvine B., Urdea M. S., Kuo G., Choo Q.-L., Houghton M. 1991; Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5′-untranslated region and poly A tails at the 3′-end. Proceedings of the National Academy of Sciences, U,. S,. A. 88:1711–1715
    [Google Scholar]
  11. He L. F., Alling D., Popkin T., Shapiro M., Alter H. J., Purcell R. H. 1987; Determining the size of non-A, non-B hepatitis virus by filtration. Journal of Infectious Diseases 156:636–640
    [Google Scholar]
  12. Horzinek M. 1973; The structure of togaviruses. Progress in Medical Virology 16:109–156
    [Google Scholar]
  13. Jacob J. R., Burk K. H., Eichberg J. W., Dreesman G. R., Lanford R. E. 1990; Expression of infectious viral particles by primary chimpanzee hepatocytes isolated during the acute phase of non-A, non-B hepatitis. Journal of Infectious Diseases 161:1121–1127
    [Google Scholar]
  14. Kamide K., Manabe S., Nohmi T., Makino H., Narita H., Kawai T. 1982; Mechanism of gas permeation through porous polymeric membrane. In Polymeric Separation Media pp. 35–74 Edited by Cooper A. R. New York & London: Plenum Press;
    [Google Scholar]
  15. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. 1990; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proceedings of the National Academy of Sciences, U,. S,. A 87:9524–9528
    [Google Scholar]
  16. Kubo Y., Takeuchi K., Boonmar S., Katayama T., Choo Q.-L., Kuo G., Weiner A. J., Bradley D. W., Houghton M., Saito I., Miyamura T. 1989; A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non A, non-B hepatitis in Japan. Nucleic Acids Research 17:10367–10372
    [Google Scholar]
  17. Kuo G., Choo Q.-L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E., Tegtmeier G. E., Bonino F., Colombo M., Lee W. S., Kuo C., Berger K., Shuster J. R., Overby L. R., Bradley D. W., Houghton M. 1989; An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362–364
    [Google Scholar]
  18. Manabe S., Iwata M., Inoue M. 1986; Porous regenerated cellulose hollow fiber and process for preparation thereof. August 1986. U.S. Patent4604326
    [Google Scholar]
  19. Manabe S., Tsurumi T., Ishikawa G., Satani M., Yamashiki T., Hamamoto Y., Yamaguchi K., Kobayashi S., Yamamoto N. 1989; Mechanism of human immunodeficiency virus (HIV) removal by regenerated cellulose hollow fiber (BMM). Membrane 14:77–83
    [Google Scholar]
  20. Mayr A., Bachmann P. A., Sheffy B. E., Siegl G. 1967; Electron optical and buoyant density studies of hog cholera virus. Archili fur die gesamte Virusforschung 21:113–119
    [Google Scholar]
  21. Miller R. H., Purcell R. H. 1990; Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proceedings of the National Academy of Sciences, U,. S,. A 87:2057–2061
    [Google Scholar]
  22. Miyamura T., Saito I., Katayama T., Kikuchi S., Tateda A., Houghton M., Choo Q.-L., Kuo G. 1990; Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis. Proceedings of the National Academy of Sciences, U,. S,. A. 87:241–246
    [Google Scholar]
  23. Monath T. P. 1985; Flaviviruses. In Virology pp. 931–933 Edited by Fields B. N., Knipe D. M., Chanock R. M., Melnick J. L., Roizman B., Shope R. E. New York: Raven Press;
    [Google Scholar]
  24. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  25. Sekiguchi S., Ito K., Kobayashi M., Ikeda H., Tsurumi T., Ishikawa G., Manabe S., Satani M., Yamashiki S. 1989; Possibility of hepatitis B virus (HBV) removal from human plasma using regenerated cellulose hollow fiber (BMM). Membrane 14:253–261
    [Google Scholar]
  26. Takamizawa A., Mori C., Fuke I., Manabe S., Murakami S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. 1991; Structure and organization of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65:1105–1113
    [Google Scholar]
  27. Takeuchi K., Kubo Y., Boonmar S., Watanabe Y., Katayama T., Choo Q.-L., Kuo G., Houghton M., Saito I., Miyamura T. 1990; The putative nucleocapsid and envelope protein genes of hepatitis C virus determined by comparison of the nucleotide sequences of two isolates derived from an experimentally infected chimpanzee and healthy human carriers. Journal of General Virology 71:3027–3033
    [Google Scholar]
  28. Tsurumi T., Osawa N., Hirasaki T., Yamaguchi K., Manabe S., Yamashiki S. 1989; Mechanism of removing monodisperse gold particles from suspension using cuprammonium regenerated cellulose hollow fiber (BMM hollow fiber). Polymer Journal 22:304–311
    [Google Scholar]
  29. Tsurumi T., Sato T., Osawa N., Hitaka H., Hirasaki H., Yamaguchi K., Hamamoto Y., Manabe S., Yamashiki T., Yamamoto N. 1990; Structure and filtration performances of improved cuprammonium regenerated cellulose hollow fiber (improved BMM hollow fiber) for virus removal. Polymer Journal 22:1085–1100
    [Google Scholar]
  30. Weiner A. J., Kuo G., Bradley D. W., Bonino F., Saracco G., Lee C., Rosenblatt J., Choo Q.-L., Houghton M. 1990; Detection of hepatitis C virus sequences in non-A, non-B hepatitis. Lancet i:1–3
    [Google Scholar]
  31. Yoneyama T., Takeuchi K., Watanabe Y., Harada H., Ohbaya-shi A., Tanaka Y., Yuasa T., Saito I., Miyamura T. 1990; Detection of hepatitis C virus cDNA sequence by the polymerase chain reaction in hepatocellular carcinoma tissues. Japanese Journal of Medical Science and Biology 43:89–94
    [Google Scholar]
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