1887

Abstract

The replicative form (RF) DNA of chicken anaemia agent (CAA) was isolated and cloned into bacterial plasmids. After religation of the cloned CAA DNA and transfection into MDCC-MSB1 cells, the DNA could induce c.p.e. characteristic of that caused by CAA, and an antigen was produced which gave positive immunofluorescence when detected with an anti-CAA serum. Sanger sequencing of the 2298 bp genome revealed several open reading frames (ORFs); the major ORF encoded a polypeptide of 51.8K. In SDS-PAGE of CAA viral particles a 50K protein has been reported as the only detectable viral protein. The genomic region downstream of the major ORF had several predicted GC-rich inverted repeats, a poly(A) signal and four copies of an 18 bp repeat element. Database searches did not reveal any sequence with homology to the viral genomic DNA, nor to the amino acid sequence of any of the ORFs, apart from the N-terminal 40 amino acids of the major ORF which showed a limited similarity to the structure of protamines.

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1991-08-01
2021-10-25
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References

  1. Birnstiel M. L., Busslinger M., Strub K. 1985; Transcription termination and 3′ processing: the end is in site!. Cell 41:349–359
    [Google Scholar]
  2. Fitzgerald M., Shenk T. 1981; The sequence of 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs. Cell 24:251–260
    [Google Scholar]
  3. Gavel Y., von Heijne G. 1990; Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. Protein Engineering 3:433–442
    [Google Scholar]
  4. Gelderblom H., Kling S., Lurz R., Tischer I., von Bülow V. 1989; Morphological characterization of chicken anaemia agent (CAA). Archives of Virology 109:115–120
    [Google Scholar]
  5. Goryo M., Suwa T., Matsumoto S., Umemura T., Itakura C. 1987; Serial propagation and purification of chicken anaemia agent in MDCC-MSB1 cell line. Avian Pathology 16:149–163
    [Google Scholar]
  6. Kozak M. 1987; An analysis of 5′-non-coding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8148
    [Google Scholar]
  7. McNulty M. S., Connor T. J., McNeilly F., Spackman D. 1989; Chicken anemia agent in the United States: isolation of the virus and detection of antibody in broiler breeder flocks. Avian Diseases 33:691–694
    [Google Scholar]
  8. McNulty M. S., Curran W. L., Todd D., Mackie D. P. 1990; Chicken anemia agent: an electron microscopic study. Avian Diseases 34:736–743
    [Google Scholar]
  9. Sambrook J., Fritsch E. F., Maniatis T. 1989Molecular Cloning: A Laboratory Manual, 2nd. edn New York: Cold Spring Harbor Laboratory;
  10. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  11. Tischer I., Gelderblom H., Vetterman W., Koch M. A. 1982; A very small porcine virus with circular single-stranded DNA. Nature, London 295:64–66
    [Google Scholar]
  12. Todd D., Creelan J. L., Mackie D. P., Rixon F., McNulty M. S. 1990; Purification and biochemical characterization of chicken anaemia agent. Journal of General Virology 71:819–823
    [Google Scholar]
  13. Yuasa N., Taniguchi T., Yoshida I. 1979; Isolation and some characteristics of an agent inducing anemia in chicks. Avian Diseases 23:366–385
    [Google Scholar]
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