The syncytial mutant of herpes simplex virus type 1 (HSV-1), HSV-1(13) S11, which carries three distinct syncytial mutations, 1, 5 and 6, was described previously. 1 maps to the HI L fragment, map units (m.u.) 0.707 to 0.745; 5 is located within the HI Q fragment, m.u. 0·296 to 0·317; 6 lies in the junction fragment HI SP, m.u. 0·81 to 0·85. Although 1 of HSV-1(13) S11 seems to be homologous to that of HSV-1(MP) and other syncytial mutants, and 5 has been recently characterized, 6 represents a novel syncytial locus which has yet to be characterized. In this paper we report the fine mapping of the 6 locus. This mutation has been mapped, by marker rescue and marker transfer experiments, to the long repeat regions (R) at both ends of the L component of the HSV genome in a restriction endonuclease fragment of approximately 1·6 kb designated HI-I C (approximate m.u. 0·01 to 0·02 and 0·81 to 0·82). In the internal copy of R the sequences containing the 6 mutation were bounded to the left by the 5′ end of the α gene specifying ICP0 and to the right by the γ1 gene encoding ICP34.5.


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