1887

Abstract

The antiviral action of cyclopentenone prostaglandins (PGs) is generally associated with alterations in the synthesis and/or maturation of specific virus proteins. In particular, inhibition of Sendai virus (SV) replication in African green monkey kidney cells by PGA has been shown to be a cell-mediated event, due to alterations in SV protein glycosylation and accompanied by the induction of a cellular polypeptide of 74K. In this report we identify this protein as a heat shock protein (HSP) related to the major 70K HSP group (HSP70). Induction of HSP70 synthesis by PGA was found to be dose-dependent, and an accumulation of HSP70 comparable to that occurring after heat shock could be obtained at concentrations of PGA that did not inhibit macromolecular synthesis in uninfected cells, but caused a dramatic block of virus replication in SV-infected cells. Induction of HSP70 by PGA occurred at the transcriptional level and was not affected by SV infection. HSP70 synthesis was evident between 2 and 3 h after PGA treatment, maximal at 12 h and went back to control levels by 26 h after the addition of PGA, thus preceding virus protein synthesis. Finally, of several PGs tested, only those which possess antiviral activity induced the synthesis of HSP70. These results, together with the observation that suppression of HSP70 synthesis by actinomycin D also abolishes the PGA-induced alteration of SV glycoproteins, suggest that HSP70 could play a role in the block of virus replication by cyclopentenone PGs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-8-1877
1991-08-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/8/JV0720081877.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-8-1877&mimeType=html&fmt=ahah

References

  1. Ananthan J., Goldberg A. L., Voellmy R. 1986; Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes. Science 232:522–524
    [Google Scholar]
  2. Ankel H., Mittnacht S., Jacobsen H. 1985; Antiviral activity of prostaglandin A on encephalomyocarditis virus-infected cells: a unique effect unrelated to interferon. Journal of General Virology 66:2355–2364
    [Google Scholar]
  3. Benavente J., Esteban M., Jaffe B. M., Santoro M. G. 1984; Selective inhibition of viral gene expression as the mechanism of the antiviral action of PGA, in vaccinia virus-infected cells. Journal of General Virology 65:599–608
    [Google Scholar]
  4. Bond U., Schlesinger M. J. 1987; Heat-shock proteins and development. Advances in Genetics 24:1–29
    [Google Scholar]
  5. Burdon R. H. 1982; The human heat-shock proteins: their induction and possible intracellular functions. In Heat Shock from Bacteria to Man pp. 283–288 Edited by Schlesinger M. J., Ashbumer M., Tissiéres A. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  6. Burnette W. N. 1981; ‘Western blotting’: electrophoretic transfer from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radio-iodinated Protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  7. Choppin P. W., Scheid A. 1980; The role of viral glycoproteins in adsorption, penetration and pathogenicity of viruses. Reviews of Infectious Diseases 2:40–61
    [Google Scholar]
  8. Collins P. L., Hightower L. 1982; Newcastle disease virus stimulates the cellular accumulation of stress (heat shock) proteins. Journal of Virology 44:703–707
    [Google Scholar]
  9. D’Onofrio C., Alvino E., Garaci E., Bonmassar E., Santoro M. G. 1990a; Selection of HTLV-I positive clones is prevented by prostaglandin A in infected cord blood cultures. British Journal of Cancer 61:207–214
    [Google Scholar]
  10. D’Onofrio C., Amici C., Bonmassar E., Santoro M. G. 1990b; The antiproliferative effect of prostaglandins A and J on HTLV-I transformed cells is associated with induction of a heat shock protein. Pharmacological Research 22:41–42
    [Google Scholar]
  11. Fukushima M., Kato I., Narumiya S., Mizushima Y., Sasaki H., Terashima Y., Nishiyama Y., Santoro M. G. 1989; Prostaglandins A and J: antitumor and antiviral prostaglandins. In Advances in Prostaglandins, Thromboxane and Leukotriene Research vol 19 pp. 415–418 Edited by Samuelsson B., Wong P. Y., Sun F. F. New York: Raven Press;
    [Google Scholar]
  12. Ham E. A., Oien H. G., Ulm E. H., Kuehl F. A. Jr 1975; The reaction of PGA, with sulfhydryl groups: a component in the binding of A-type prostaglandins to proteins. Prostaglandins 10:217–223
    [Google Scholar]
  13. Hsu M., Scheid A., Choppin P. W. 1979; Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution. Virology 95:476–491
    [Google Scholar]
  14. Hunt C., Morimoto R. I. 1985; Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70. Proceedings of the National Academy of Sciences, U,. S,. A 82:6455–6459
    [Google Scholar]
  15. Kao H., Nevins J. R. 1983; Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection. Molecular and Cellular Biology 3:2058–2065
    [Google Scholar]
  16. Karmali R., Schiller P., Horrobin D. F. 1976; Prostaglandins can prevent the binding of chloroquine to calf thymus DNA. Prostaglandins 12:463–469
    [Google Scholar]
  17. Khandjian E. W., Turler H. 1983; Simian virus 40 and polyoma virus induce the synthesis of heat shock proteins in permissive cells. Molecular and Cellular Biology 3:1–8
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  19. Lindquist S., Craig E. A. 1988; The heat-shock proteins. Annual Review of Genetics 22:631–677
    [Google Scholar]
  20. Mastromarino P., Conti C., Petruzziello R., Pica F., Santoro M. G. 1990; Inhibition of togavirus replication by prostaglandin A. VIIIth International Congress of Virology Abstracts p 319
    [Google Scholar]
  21. Morimoto R. I., Abravaya K., Mosser D., Williams G. T. 1990; Transcription of the human hsp70 gene: cis-acting elements and trans-acting factors involved in basal, adenovirus El A, and stress-induced expression. In Stress Proteins: Induction and Function pp. 1–17 Edited by Schlesinger M. J., Santoro M. G., Garaci E. Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  22. Notarianni E. L., Preston C. M. 1982; Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce polypeptides. Virology 123:113–122
    [Google Scholar]
  23. Pelham H. R. 1986; Speculations on the function of the major heat shock and glucose-regulated proteins. Cell 46:959–961
    [Google Scholar]
  24. Peluso R. W., Lamb R. A., Choppin P. W. 1978; Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose. Proceedings of the National Academy of Sciences, U,. S,. A 75:6120–6124
    [Google Scholar]
  25. Russell J., Stow E. C., Stow N. D., Preston C. M. 1987; Abnormal forms of the herpes simplex virus immediate early polypeptide Vmwl75 induce the cellular stress response. Journal of General Virology 68:2397–2406
    [Google Scholar]
  26. Santoro M. G. 1987; Involvement of protein synthesis in the antiproliferative and the antiviral action of prostaglandins. In Prostaglandins in Cancer Research pp. 97–114 Edited by Garaci E., Paoletti R., Santoro M. G. Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  27. Santoro M. G., Benedetto A., Carruba G., Garaci E., Jaffe B. M. 1980; Prostaglandin A compounds as antiviral agents. Science 209:1032–1034
    [Google Scholar]
  28. Santoro M. G., Carruba G., Garaci E., Jaffe B. M., Benedetto A. 1981; Prostaglandins of the A series inhibit Sendai virus replication in cultured cells. Journal of General Virology 53:75–83
    [Google Scholar]
  29. Santoro M. G., Jaffe B. M., Garaci E., Esteban M. 1982a; Antiviral effect of prostaglandins of the A series: inhibition of vaccinia virus replication in cultured cells. Journal of General Virology 63:435–440
    [Google Scholar]
  30. Santoro M. G., Jaffe B. M., Elia G., Benedetto A. 1982b; Prostaglandin A1 induces the synthesis of a new protein in cultured AGMK cells. Biochemical and Biophysical Research Communications 107:1179–1184
    [Google Scholar]
  31. Santoro M. G., Jaffe B. M., Esteban M. 1983; Prostaglandin A inhibits the replication of vesicular stomatitis virus: effect on virus glycoprotein. Journal of General Virology 64:2797–2801
    [Google Scholar]
  32. Santoro M. G., Crisari A., Benedetto A., Amici C. 1986; Modulation of the growth of a human erythroleukemic cell line (K562) by prostaglandins: antiproliferative action of PGAs. Cancer Research 46:6073–6077
    [Google Scholar]
  33. Santoro M. G., Fukushima M., Benedetto A., Amici C. 1987; PGJ2, a new antiviral prostaglandin: inhibition of Sendai virus replication and alteration of virus protein synthesis. Journal of General Virology 68:1153–1158
    [Google Scholar]
  34. Santoro M. G., Favalli C., Mastino A., Jaffe B. M., Esteban M., Garaci E. 1988; Antiviral activity of a synthetic analog of prostaglandin A in mice infected with influenza A virus. Archives of Virology 99:89–100
    [Google Scholar]
  35. Santoro M. G., Amici C., Elia G., Benedetto A., Garaci E. 1989a; Inhibition of virus protein glycosylation as the mechanism of the antiviral action of prostaglandin A in Sendai virus-infected cells. Journal of General Virology 70:789–800
    [Google Scholar]
  36. Santoro M. G., Garaci E., Amici C. 1989b; Prostaglandins with antiproliferative activity induce the synthesis of a heat shock protein in human cells. Proceedings of the National Academy of Sciences, U.S.A 86:8407–8411
    [Google Scholar]
  37. Schlesinger M. J. 1990; Heat shock proteins. Journal of Biological Chemistry 265:12111–12114
    [Google Scholar]
  38. Schlesinger M. J., Santoro M. G., Garaci E. 1990 Stress Proteins: Induction and Function Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  39. Yamamoto N., Rahman M., Fukushima M., Maeno K., Nishiyama Y. 1989; Involvement of prostaglandin-induced proteins in the inhibition of herpes simplex virus replication. Biochemical and Biophysical Research Communications 158:189–194
    [Google Scholar]
  40. Yura Y., Terashima K., Iga H., Kondo Y., Yanagawa T., Yoshida H., Hiyashi Y., Sato M. 1987; Macromolecular synthesis at the early stage of herpes simplex type 2 (HSV 2) latency in a human neuroblastoma cell line IMR-32: repression of late viral polypeptide synthesis and accumulation of cellular heat-shock proteins. Archives of Virology 96:17–28
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-8-1877
Loading
/content/journal/jgv/10.1099/0022-1317-72-8-1877
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error