RT Journal Article SR Electronic(1) A1 Aboagye-Mathiesen, George A1 Tóth, Ferenc D. A1 Juhl, Claus A1 Nørskov-Lauritsen, Niels A1 Petersen, Peter M. A1 Zachar, Vladimir A1 Ebbesen, PeterYR 1991 T1 Characterization Of Sendai Virus-Induced Human Placental Trophoblast Interferons JF Journal of General Virology, VO 72 IS 8 SP 1871 OP 1876 DO https://doi.org/10.1099/0022-1317-72-8-1871 PB Microbiology Society, SN 1465-2099, AB Human placental trophoblast cultures produce a mixture of interferons (IFNs) when challenged with Sendai virus. High-performance dye-ligand and immunoaffinity chromatography of a trophoblast IFN (tro-IFN) preparation enabled the isolation of three antigenically distinct IFNs, α I, α II1 and β, with M rs of 16K, 22K and 24K respectively, by reducing and non-reducing SDS-PAGE. The major IFN, responsible for 75% of the total antiviral activity, was tro-IFN-β, with the remaining activity being due to tro-IFN-α I and tro-IFN-α II1, as determined by an antiviral neutralization test using specific anti-human IFN antibodies. The antiviral activities of the tro-IFNs were stable at pH 2.0 for 24 h and tro-IFN-α II1 and -β were shown to be glycoproteins. The three tro-IFNs showed different antiviral activities when assayed on human and bovine cell species; tro-IFN-α I and -α II1 protected both human and bovine (MDBK) cells from virus infection, whereas tro-IFN-β showed a high degree of species specificity, protecting only the human cell types tested., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-8-1871