1887

Abstract

Human placental trophoblast cultures produce a mixture of interferons (IFNs) when challenged with Sendai virus. High-performance dye-ligand and immunoaffinity chromatography of a trophoblast IFN (tro-IFN) preparation enabled the isolation of three antigenically distinct IFNs, , 1 and , with s of 16K, 22K and 24K respectively, by reducing and non-reducing SDS-PAGE. The major IFN, responsible for 75% of the total antiviral activity, was tro-IFN-, with the remaining activity being due to tro-IFN- and tro-IFN- 1, as determined by an antiviral neutralization test using specific anti-human IFN antibodies. The antiviral activities of the tro-IFNs were stable at pH 2.0 for 24 h and tro-IFN- 1 and - were shown to be glycoproteins. The three tro-IFNs showed different antiviral activities when assayed on human and bovine cell species; tro-IFN- and - 1 protected both human and bovine (MDBK) cells from virus infection, whereas tro-IFN- showed a high degree of species specificity, protecting only the human cell types tested.

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1991-08-01
2024-03-29
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