1887

Journal of General Virology header logo

Volume 72, Issue 8

The Nucleotide Sequence and Luteovirus-like Nature of RNA 1 of an Aphid Non-transmissible Strain of Pea Enation Mosaic Virus Free

Abstract

An examination of the genomic strategy of pea enation mosaic virus (PEMV) RNA 1 has verified strong organizational and sequence relationships between PEMV and the beet western yellows-potato leafroll luteovirus subgroup. Sequence analysis of RNA 1 demonstrated five predominant open reading frames (ORFs). The extreme 5′ ORF encodes a 34K product of unknown function. The second ORF encodes an 84K product which overlaps 90% of ORF 1 (in a unique reading frame) and is expressed by internal initiation beginning at the second start codon from the 5′ terminus. This protein contains a protease-like motif characteristic of serine- and cysteine-based proteases, suggesting involvement in post-translational processing of viral translation products. The third ORF is characterized by a number of RNA polymerase motifs and a helicase-like motif typical of RNA-dependent RNA polymerases. It overlaps (out of frame) the ORF 2 product and is proposed to be expressed by a frameshift fusion of the ORF 2 and ORF 3 products. The fourth ORF encodes the viral coat protein, and is immediately followed in frame by a 33K ORF thought to represent the aphid transmission subunit of the PEMV virion. Northern blot analysis of polysome-associated RNA suggests that both products are expressed from an 1800 nucleotide subgenomic mRNA, with the 33K product expressed as a read-through fusion with the coat protein monomer.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1991
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-8-1819
1991-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/8/JV0720081819.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-8-1819&mimeType=html&fmt=ahah

References

  1. Adam G., Sander E., Shepherd R. J. 1979; Structural differences between pea enation mosaic virus strains affecting transmissibility by Acyrthosiphon pisum (Harris). Virology 92:1–14
     [Google Scholar]
  2. Allison R. F., Janda M., Ahlquist P. 1988; Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus. Journal of Virology 62:3581–3588
     [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. F., Moore D. D., Smith J. A., Seidman J. G., Struhl K. (editors) 1987 Current Protocols in Molecular Biology New York: J. Wiley & Sons;
     [Google Scholar]
  4. Bahner I., Lamb J., Mayo M. A., Hay R. T. 1990; Expression of the genome of potato leafroll virus : readthrough of the coat protein termination codon in vivo . Journal of General Virology 71:2251–256
     [Google Scholar]
  5. Bazan J. F., Fletterick R. J. 1988; Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications. Proceedings of the National Academy of Sciences, U.S.A. 85:7872–7876
     [Google Scholar]
  6. Chin L. S., Blish C. A., Falk B. W. 1990; Cell-free translation analysis of the beet western yellows virus ST9 isolate virion RNAs and in vitro transcripts of capsid protein ORF cDNA clones. (Abstract). American Phytopathological Society Annual Meeting
     [Google Scholar]
  7. Cockbain A. J., Jones P., Woods R. D. 1986; Transmission characteristics and some other properties of bean yellow veinbanding virus and its association with pea enation mosaic virus. Annals of Applied Biology 108:59–70
     [Google Scholar]
  8. Demler S. A., de Zoeten G. A. 1984; Characterization of a satellite RNA associated with pea enation mosaic virus. Journal of General Virology 70:1075–1084
     [Google Scholar]
  9. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–390
     [Google Scholar]
  10. de Zoeten G. A., Gaard G. 1983; Mechanisms underlying systemic invasion of pea plants by pea enation mosaic virus. Intervirology 19:85–94
     [Google Scholar]
  11. de Zoeten G. A., Gaard G., Diez F. B. 1972; Nuclear vesiculation associated with pea enation mosaic virus-infected plant tissue. Virology 48:638–647
     [Google Scholar]
  12. de Zoeten G. A., Powell C. A., Gaard G., German T. L. 1976; In situ localization of pea enation mosaic virus double-stranded ribonucleic acid. Virology 70:459–469
     [Google Scholar]
  13. Domier L. L., Shaw J. G., Rhoads R. E. 1987; Potyviral proteins share amino acid sequence homology with picoma-, comoand caulimoviral proteins. Virology 158:20–27
     [Google Scholar]
  14. Doolittle R. F. 1981; Similar amino acid sequences: chance or common ancestry?. Science 214:149–159
     [Google Scholar]
  15. England T. E., Bruce A. G., Uhlenbeck O. C. 1980; Specific labeling of 3′ termini of RNA with T4 RNA ligase. Methods in Enzymology 65:65–74
     [Google Scholar]
  16. Esau K., Hoefert L. L. 1972; Development of infection with beet western yellows virus in the sugarbeet. Virology 48:724–738
     [Google Scholar]
  17. Falk B. W., Duffus J. E. 1984; Identification of small single- and double-stranded RNAs associated with severe symptoms in beet western yellows virus-infected plants. Phytopathology 74:1224–1229
     [Google Scholar]
  18. Falk B. W., Chin L. -S., Duffus J. E. 1989; Complementary DNA cloning and hybridization analysis of beet western yellows luteovirus RNAs. Journal of General Virology 70:1301–1309
     [Google Scholar]
  19. Gabriel C. J. 1983 Investigations into the in vitro translation and aphid transmission of pea enation mosaic virus Ph,. D. thesis, University of Wisconsin, Madison;
     [Google Scholar]
  20. Gabriel C. J., de Zoeten G. A. 1984; The in vitro translation of pea enation mosaic virus RNA. Virology 139:223–230
     [Google Scholar]
  21. Gerlach W. L., Miller W. A., Waterhouse P. M. 1987; Molecular genetics of barley yellow dwarf virus. Barley Yellow Dwarf Newsletter 1:17–19
     [Google Scholar]
  22. German T. L., de Zoeten G. A. 1975; Purification and properties of the replicative forms and replicative intermediates of pea enation mosaic virus. Virology 66:172–184
     [Google Scholar]
  23. German T. L., de Zoeten G. A., Hall T. C. 1978; Pea enation mosaic virus genome RNA contains no polyadenylate sequences and cannot be aminoacylated. Intervirology 9:226–230
     [Google Scholar]
  24. Giege R., Briand J., Mengual R., Ebel J., Hirth L. 1978; Valylation of the two RNA components of turnip-yellow mosaic virus and specificity of the tRNA aminoacylation reaction. European Journal of Biochemistry 84:251–256
     [Google Scholar]
  25. Gildow F. E. 1982; Coated-vesicle transport of luteoviruses through salivary glands of Myzus persicae . Phytopathology 72:1289–1296
     [Google Scholar]
  26. Gildow F. E., Rochow W. F. 1980a; Role of accessory salivary glands in aphid transmission of barley yellow dwarf virus. Virology 104:97–108
     [Google Scholar]
  27. Gildow F. E., Rochow W. F. 1980b; Transmission interference between two isolates of barley yellow dwarf virus in Macrosiphum avenae . Phytopathology 70:122–126
     [Google Scholar]
  28. Gildow F. E., Rochow W. F. 1980c; Importance of capsid integrity for interference between two isolates of barley yellow dwarf virus in an aphid. Phytopathology 70:1013–1015
     [Google Scholar]
  29. Gonsalves D., Shepherd R. J. 1972; Biological and physical properties of the two nucleoprotein components of pea enation mosaic virus and their associated nucleic acids. Virology 48:709–723
     [Google Scholar]
  30. Gorbalenya A. E., Koonin E. V. 1990; Peculiarities in the catalytic centre design of positive strand RNA virus 3C-like proteases. VIII International Congress of Virology Abstracts
     [Google Scholar]
  31. Gorbalenya A. E., Donchenko A. P., Blinov V. M., Koonin E. V. 1989; Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. FEBS Letters 243:103–114
     [Google Scholar]
  32. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–268
     [Google Scholar]
  33. Habili N., Symons R. H. 1989; Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases. Nucleic Acids Research 17:9543–9555
     [Google Scholar]
  34. Harris K. F., Bath J. E. 1972; The fate of pea enation mosaic virus in its pea aphid vector, Acyrthosiphon pisum (Harris). Virology 50:778–790
     [Google Scholar]
  35. Harris K. F., Bath J. E., Thottappilly G., Hooper G. R. 1975; Fate of pea enation mosaic virus in PEMV-injected pea aphids. Virology 65:148–162
     [Google Scholar]
  36. Hatfield D. L., Smith D. W. E., Lee B. J., Worland P. J., Oroszlan S. 1990; Structure and function of suppressor tRNAs in higher eucaryotes. CRC Critical Reviews in Biochemistry and Molecular Biology 25:71–96
     [Google Scholar]
  37. Hodgman T. C. 1988; A new family of replicative proteins. Nature, London 333:22–23
     [Google Scholar]
  38. Hull R. 1977a; Properties of an aphid-borne virus: pea enation mosaic virus. In Aphids as Virus Vectors pp. 137–162 Edited by Harris K. F., Maramorosch K. New York: Academic Press;
     [Google Scholar]
  39. Hull R. 1977b; Particle differences related to aphid-transmissibility of a plant virus. Journal of General Virology 34:183–187
     [Google Scholar]
  40. Hull R., Lane L. C. 1973; The unusual nature of the components of a strain of pea enation mosaic virus. Virology 55:1–13
     [Google Scholar]
  41. Jacks T., Madhani H. D., Masiarz F. R., Varmus H. E. 1988; Signals for ribosomal frameshifting in the Rous sarcoma virus gagpol region. Cell 55:447–458
     [Google Scholar]
  42. Jackson A. O., Larkins B. A. 1976; Influence of ionic strength, pH, and chelation of divalent metals on isolation of polyribosomes from tobacco leaves. Plant Physiology 57:5–10
     [Google Scholar]
  43. Joshi R. L., Joshi S., Chappeville F., Haenni A. L. 1983; tRNA-like structures of plant viral RNAs: conformational requirements for adenylation and aminoacylation. EMBO Journal 2:1123–1127
     [Google Scholar]
  44. Keese P., Martin R. R., Kawchuk L. M., Waterhouse P. M., Gerlach W. L. 1990; Nucleotide sequences of an Australian and a Canadian isolate of potato leafroll luteovirus and their relationships with two European isolates. Journal of General Virology 71:719–724
     [Google Scholar]
  45. Kozak M. 1989; The scanning model for translation: an update. Journal of Cell Biology 108:229–241
     [Google Scholar]
  46. Krupp G., Gross H. 1983; Sequence analysis of in vitro 32P-labeled RNA. In The Modified Nucleosides of Transfer RNA II pp. 11–58 Edited by Agris P. F., Kopper R. A. New York: Alan R. Liss;
     [Google Scholar]
  47. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
     [Google Scholar]
  48. Lentz T. L. 1990; The recognition event between virus and host cell receptor: a target for antiviral agents. Journal of General Virology 71:751–766
     [Google Scholar]
  49. Lutcke H., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. 1987; Selection of AUG initiation codons differs in plants and animals. EMBO Journal 6:43–48
     [Google Scholar]
  50. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  51. Mayo M. A., Robinson D. J., Jolly C. A., Hyman L. 1989; Nucleotide sequence of potato leafroll luteovirus RNA. Journal of General Virology 70:1037–1051
     [Google Scholar]
  52. Melcher U. 1990; Similarities between putative transport proteins of plant viruses. Journal of General Virology 71:1009–1018
     [Google Scholar]
  53. Miller W. A., Waterhouse P. M., Gerlach W. L. 1988a; Sequence and organization of barley yellow dwarf virus genomic RNA. Nucleic Acids Research 16:6097–6111
     [Google Scholar]
  54. Miller W. A., Waterhouse P. M., Kortt A. A., Gerlach W. L. 1988b; Sequence and identification of the barley yellow dwarf virus coat protein gene. Virology 165:306–309
     [Google Scholar]
  55. Paje-Manalo L. L., Lommel S. A. 1989; Independent replication of red clover necrotic mosaic virus RNA-1 in electroporated host and non-host Nicotiana species protoplasts. Phytopathology 19:457–461
     [Google Scholar]
  56. Powell C. A., de Zoeten G. A. 1977; Replication of pea enation mosaic virus RNA in isolated pea nuclei. Proceedings of the National Academy of Sciences, U.S.A. 74:2919–2922
     [Google Scholar]
  57. Powell C. A., de Zoeten G. A., Gaard G. 1977; The localization of pea enation mosaic virus-induced RNA-dependent RNA polymerase in infected peas. Virology 78:135–143
     [Google Scholar]
  58. Reisman D., DE Zoeten G. A. 1982; A covalently linked protein at the 5′-ends of the genomic RNAs of pea enation mosaic virus. Journal of General Virology 62:187–190
     [Google Scholar]
  59. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
     [Google Scholar]
  60. Schwartz R. M., Dayhoff M. O. 1979; Matrices for detecting distinct relationships. In Atlas of Protein Sequence and Structure pp. 353–358 Edited by Dayhoff M. O. Washington, D.C.: National Biomedical Research Foundation;
     [Google Scholar]
  61. Shepardson S., Esau K., McCrum R. 1980; Ultrastructure of potato leaf phloem infected with potato leafroll virus. Virology 105:379–390
     [Google Scholar]
  62. Shepherd R. J., Wakeman R. J., Ghabrial S. A. 1968; Preparation and properties of the protein and nucleic acid components of pea enation mosaic virus. Virology 35:255–267
     [Google Scholar]
  63. Silflow C. D., Hammet J. R., Key J. L. 1979; Sequence complexity of polyadenylated ribonucleic acid from soybean suspension culture cells. Biochemistry 18:2725–2731
     [Google Scholar]
  64. Smith O. P., Harris K. F. 1990; Potato leafroll virus 3′ genome organization: sequence of the coat protein gene and identification of a viral subgenomic RNA. Phytopathology 80:609–614
     [Google Scholar]
  65. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences, U.S.A. 84:4767–4771
     [Google Scholar]
  66. Tacke E., Prufer D., Salamini F., Rodhe W. 1990; Characterization of a potato leafroll luteovirus subgenomic RNA: differential expression by internal translation initiation and UAG suppression. Journal of General Virology 71:2265–2272
     [Google Scholar]
  67. Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K., Jonard G. 1988; Nucleotide sequence of beet western yellows virus RNA. Nucleic Acids Research 16:9917–9932
     [Google Scholar]
  68. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:15–23
     [Google Scholar]
  69. Waterhouse P. M., Martin R. R., Gerlach W. L. 1989; BYDV-PAV virions contain readthrough protein. (Abstract). Phytopathology 79:1215
     [Google Scholar]
  70. Waterhouse P., Martin R. R., Keese P., Young M., Gerlach W. 1990; Molecular analysis of the genes involved with aphid transmission of luteoviruses. VIII International Congress of Virology Abstracts
     [Google Scholar]
  71. Wengler G., Wengler G. 1981; Terminal sequences of the genome and replicative form RNA of the flavivirus West Nile virus: absence of poly(A) and possible role in RNA replication. Virology 113:544–555
     [Google Scholar]
  72. Xiong Z., Lommel S. A. 1989; The complete nucleotide sequence and genome organization of red clover necrotic mosaic virus RNA-1. Virology 171:543–554
     [Google Scholar]
  73. Young M. J., Kelly L., Larkin P. J., Waterhouse P. M., Gerlach W. L. 1991; Infectious in vitro transcripts form a cloned cDNA of barley yellow dwarf virus. Virology 180:372–379
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-8-1819
Loading
The Nucleotide Sequence and Luteovirus-like Nature of RNA 1 of an Aphid Non-transmissible Strain of Pea Enation Mosaic Virus
J. Gen. Virol. 72, 1819 (1991); https://doi.org/10.1099/0022-1317-72-8-1819
/content/journal/jgv/10.1099/0022-1317-72-8-1819
/content/journal/jgv/10.1099/0022-1317-72-8-1819
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error