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Abstract

The cytoplasmically inherited killer virus of expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15 °C . In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.

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/content/journal/jgv/10.1099/0022-1317-72-7-1755
1991-07-01
2025-11-07

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References

  1. Ball L. A., White C. N. 1976; Order of transcription of vesicular stomatitis virus. Proceedings of the National Academy of Sciences, U,. S,. A 73:442–446
    [Google Scholar]
  2. Bostian K. A., Hopper J. K., Rogers D. J., Tipper D. J. 1980; Translational analysis of the killer-associated virus-like particle dsRNA genome of S. cerevisiae. M-dsRNA encodes toxin. Cell 19:403–414
    [Google Scholar]
  3. Bostian K. A., Burn J. E., Jayachandran S., Tipper D. J. 1983; Yeast killer dsRNA plasmids are transcribed in vivo to produce full and partial-length plus stranded RNAs. Nucleic Acids Research 11:1077–1097
    [Google Scholar]
  4. Bruenn J., Bobek L., Brennan V., Held W. 1980; Yeast viral RNA polymerase is a transcriptase. Nucleic Acids Research 8:2985–2997
    [Google Scholar]
  5. Chen H. Z., Zubay H. 1983; Procaryotic coupled transcription and translation. Methods in Enzymology 101:674–690
    [Google Scholar]
  6. Diamond M. E., Dowhanick J. J., Nemeroff M. E., Pietras D. F., Tu C., Bruenn J. A. 1989; Overlapping genes in a yeast double stranded RNA virus. Journal of Virology 63:3983–3990
    [Google Scholar]
  7. Esteban R., Wickner R. B. 1986; Three different M1 doublestranded RNA-containing viral like particle types in Saccharomyces cerevisiae : in vitro M1 double-stranded RNA synthesis. Molecular and Cellular Biology 6:1552–1561
    [Google Scholar]
  8. Fujimura T., Wickner R. B. 1988a; Gene overlaps results in a viral protein having an RNA binding domain and a major coat protein domain. Cell 55:663–671
    [Google Scholar]
  9. Fujimura T., Wickner R. B. 1988b; Replicase of L-A virus-like particles of Saccharomyces cerevisiae: in vitro conversion of exogenous L-A and M1 single-stranded RNAs to double-stranded form. Journal of Biological Chemistry 263:454–460
    [Google Scholar]
  10. Fujimura T., Esteban R., Wickner R. B. 1986; In vitro L-A double-stranded RNA synthesis in virus-like particles from Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences, U,. S,. A 83:4433–4437
    [Google Scholar]
  11. Georgopoulos D. E., Leibowitz M. J. 1984; RNA polymerase of killer virus of yeast. Annals of the New York Academy of Sciences 435:592–594
    [Google Scholar]
  12. Georgopoulos D. E., Leibowitz M. J. 1987; Nucleotide phosphotransferase, nucleotide kinase and inorganic pyrophosphatase activities of killer virions of yeast. Yeast 3:117–129
    [Google Scholar]
  13. Groves W. E., Davis F. C. Jr, Sells B. H. 1968; Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Analytical Biochemistry 22:195–210
    [Google Scholar]
  14. Hannig E. M., Thiele D. J., Leibowitz M. J. 1984; Saccharomyces cerevisiae killer virus transcripts contain templatecoded polyadenylate tracts. Molecular and Cellular Biology 4:101–109
    [Google Scholar]
  15. Hopper J. E., Bostian K. A., Rowe L. B., Tipper D. J. 1977; Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae . Journal of Biological Chemistry 252:9010–9017
    [Google Scholar]
  16. Hussain I., Leibowitz M. J. 1986; Translation of homologous and heterologous messenger RNAs in a yeast cell-free system. Gene 46:13–23
    [Google Scholar]
  17. Icho T., Wickner R. B. 1989; The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames. Journal of Biological Chemistry 264:6716–6723
    [Google Scholar]
  18. Leibowitz M. J., & Barbone F. P., Georgopoulos D. E. 1991; In vitro protein synthesis. Methods in Enzymology 194:536–545
    [Google Scholar]
  19. Lolle S., Skipper N., Bussey H., Thomas D. Y. 1984; The expression of cDNA clones of yeast Ml double-stranded RNA in yeast confers both killer and immunity phenotypes. EMBO Journal 3:1383–1387
    [Google Scholar]
  20. Maniatis T., Fritsch F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Miller O. L. Jr, Hamkalo B. A., Thomas C. A. Jr 1970; Visualization of bacterial genes in action. Science 169:392–395
    [Google Scholar]
  22. Nemeroff M. E., Bruenn J. A. 1987; Initiation by the yeast viral transcriptase in vitro . Journal of Biological Chemistry 262:6785–6787
    [Google Scholar]
  23. Pelham H. R. B., Sykes J. M. M., Hunt T. 1978; Characteristics of a coupled cell-free system directed by vaccinia cores. European Journal of Biochemistry 82:199–209
    [Google Scholar]
  24. Thiele D. J., Hannig E. M., Leibowitz M. J. 1984; Multiple L double-stranded RNA species of Saccharomyces cerevisiae: evidence for separate encapsidation. Molecular and Cellular Biology 4:92–100
    [Google Scholar]
  25. Tipper D. J., Bostian K. A. 1984; Double-stranded ribonucleic acid killer systems in yeast. Microbiological Reviews 48:125–156
    [Google Scholar]
  26. Welsh J. D., Leibowitz M. 1980; Transcription of killer virion double-stranded RNA in vitro . Nucleic Acids Research 8:2365–2375
    [Google Scholar]
  27. Welsh J. D., Leibowitz M. J., Wickner R. B. 1980; Virion DNA-independent RNA polymerase from Saccharomyces cerevisiae . Nucleic Acids Research 8:2349–2363
    [Google Scholar]
  28. Wickner R. B. 1974; “Killer character” of Saccharomyces cerevisiae: curing by growth at elevated temperature. Journal of Bacteriology 117:1356–1357
    [Google Scholar]
  29. Wickner R. B. 1986; Double-stranded RNA replication in yeast: the killer system. Annual Review of Biochemistry 55:373–395
    [Google Scholar]
  30. Williams T. L., Leibowitz T. 1987; Conservative mechanism of the in vitro transcription of killer virus of yeast. Virology 158:231–234
    [Google Scholar]
  31. Zorg J., Kilian S., Radler F. 1988; Killer toxin producing strains of the yeasts Hanseniaspora uvarum and Pichia kluyveri . Archives of Microbiology 149:261–267
    [Google Scholar]
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