1887

Abstract

The cytoplasmically inherited killer virus of expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15 °C . In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.

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/content/journal/jgv/10.1099/0022-1317-72-7-1755
1991-07-01
2022-01-18
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