Several strains of cucumber mosaic virus have been categorized as either ‘fast’ or ‘slow’, based on the time of appearance of symptoms after inoculation onto zucchini squash ( cv. Black Beauty). These strains were examined for their ability to replicate in muskmelon ( cv. Iroquois) at elevated temperatures. All of the fast strains were able to replicate at 37 °C in muskmelon, whereas all of the slow strains were unable to replicate to detectable levels at 37 °C, but replicated efficiently at 27 °C. Using previously constructed pseudorecombinants between a fast and a slow strain, Fny- and Sny-CMV, temperature sensitivity was mapped to RNA 1 of the Sny-CMV strain.


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