1887

Abstract

The distribution and clearance of viral RNA (vRNA) and mRNA has been analysed for the acute and recovery stages of the pneumonia induced by intranasal infection of C57BL/6J mice with H3N2 influenza A viruses. Amplification of viral genomic material by the polymerase chain reaction showed that the influenza haemagglutinin (HA) gene was eliminated from the lungs of immunologically intact mice by day 14 post-infection, whereas depletion of the CD4 T cells delayed clearance by at most 4 days. Viral RNA encoding the HA gene was first demonstrated in the regional mediastinal lymph nodes at 48 h, and continued to be present until day 6 or day 10 after infection of the intact and CD4-depleted mice, respectively. Evidence for the presence of vRNA in the thymus, but not in the mesenteric lymph nodes or the spleen, was found in some situations. Otherwise, the distribution and clearance of vRNA was as would be predicted from earlier studies using virus isolation procedures to monitor localization patterns, and shows a lack of long-term persistence of the influenza virus genome.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-7-1695
1991-07-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/7/JV0720071695.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-7-1695&mimeType=html&fmt=ahah

References

  1. Ada G. L., Jones P. D. 1986; The immune response in influenza infection. Current Topics in Microbiology and Immunology 128:1–54
    [Google Scholar]
  2. Allan W., Tabi Z., Cleary A., Doherty P. C. 1990; Cellular events in the lymph node and lung of mice with influenza: consequences of depleting CD4+ T cells. Journal of Immunology 144:3980–3986
    [Google Scholar]
  3. Astry C. L., Yolken R. H., Jakab J. 1984; Dynamics of viral growth, viral enzymatic activity and antigenicity in murine lungs during the course of influenza pneumonia. Journal of Medical Virology 14:81–90
    [Google Scholar]
  4. Bennink J. R., Effros R. B., Doherty P. C. 1978; Influenzal pneumonia: early appearance of cross-reactive T cells in lungs of mice primed with heterologous type A viruses. Immunology 35:503–509
    [Google Scholar]
  5. Bressoud A., Whitcomb J., Pourzand C., Haller O., Cerutti P. 1990; Rapid detection of influenza virus H1 by the polymerase chain reaction. Biochemical and Biophysical Research Communications 169:425–430
    [Google Scholar]
  6. Cane C., Dimmock N. J. 1990; Intracellular stability of the gene encoding influenza virus hemagglutinin. Virology 175:385–390
    [Google Scholar]
  7. Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintans J., Loken M. R., Pierres M., Fitch F. 1983; Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody Gkl. 5: similarity of L3T4 to Leu3/T4. Journal of Immunology 131:2445–2451
    [Google Scholar]
  8. Fazekas de St Groth S., Webster R. G. 1966; Disquisitions on original antigenic sin. I. Evidence in man. Journal of Experimental Medicine 124:331–345
    [Google Scholar]
  9. Gething M. J., Bye J., Skehel J., Waterfield J. 1980; Cloning and DNA sequence of double-stranded copies of haemagglutinin genes from H2 and H3 strains elucidates antigenic shift and drift in human influenza virus. Nature, London 287:301–306
    [Google Scholar]
  10. Gray D., Skarvall H. 1988; B-cell memory is short-lived in the absence of antigen. Nature, London 336:70–73
    [Google Scholar]
  11. Katz J. M., Webster R. G. 1988; Antigenic and structural characterization of multiple subpopulations of H3N2 influenza virus from an individual. Journal of Virology 165:446–456
    [Google Scholar]
  12. Kilbourne E. D. 1969; Future influenza vaccines and the use of genetic recombinants. Bulletin of The World Health Organization 41:643–645
    [Google Scholar]
  13. Lynch F., Doherty P. C., Ceredig R. 1989; Phenotypic and functional analysis of the cellular response in regional lymphoid tissue during an acute virus infection. Journal of Immunology 142:3592–3598
    [Google Scholar]
  14. Wang M., Webster R. G. 1990; Lack of persistence of influenza virus genetic information in ducks. Archives of Virology 111:263–268
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-7-1695
Loading
/content/journal/jgv/10.1099/0022-1317-72-7-1695
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error