1887

Abstract

Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wild-type HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.

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1991-07-01
2021-10-18
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References

  1. Andzhaparidze A. G., Kazachkov Yu. A., Balayan M. S., Kusov Yu. Yu., Poleschuk V. F. 1985; Hepatitis A in Macaca fascicularis and Macaca arctoides infected with the Yam-55 strain of hepatitis A virus. Voprosy Virusologii 1:50–53
    [Google Scholar]
  2. Andzhaparidze A. G., Balayan M. S., Savinov A. P., Kazachkov Yu. A., Titova I. P. 1987; Spontaneous hepatitis, similar to hepatitis A, in African green monkeys. Voprosy Virusologii 6:681–686
    [Google Scholar]
  3. Balayan M. S., Kusov Yu. Yu., Andzhaparidze A. G., Tsarev S. A., Sverdlov E. D., Chizhikov V. E., Blinov V. M., Vasilenko S. K. 1989; Variations in genome fragments coding for RNA polymerase in human and simian hepatitis A virus. FEBS Letters 247:425–428
    [Google Scholar]
  4. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction method for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  5. Brown E. A., Jansen R. W., Lemon S. M. 1989; Characterizations of a simian hepatitis A virus (HAV): antigenic and genetic comparison with human HAV. Journal of Virology 63:4932–4937
    [Google Scholar]
  6. Callahan P. L., Mizutani S., Colonno R. J. 1985; Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14. Proceedings of the National Academy of Sciences, U,. S,. A 82:732–736
    [Google Scholar]
  7. Cohen J. I., Ticehurst J. R., Purcell R. H., Buckler-White A., Baroudy B. M. 1987a; Complete nucleotide sequence of wild type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses. Journal of Virology 61:50–59
    [Google Scholar]
  8. Cohen J. I., Rosenblum B., Ticehurst J. R., Daemer R. J., Feinstone S. M., Purcell R. H. 1987; Complete nucleotide sequence of an attenuated hepatitis A virus: comparison with wild type virus. Proceedings of the National Academy of Sciences, U,. S,. A 84:2497–2501
    [Google Scholar]
  9. Dayhoff M. O. 1978; Survey of new data and computer methods of analysis. In Atlas of Protein Sequence and Structure vol 5 supplement 3 pp. 1–8 Edited by Dayhoff M. O. Washington, D.C.: National Biomedical Research Foundation;
    [Google Scholar]
  10. Deinhardt F., Peterson D., Gross G., Wolfe L., Holmes A. W. 1975; Hepatitis in marmosets. American Journal of Medical Sciences 270:73–80
    [Google Scholar]
  11. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  12. Dienstag J. L., Feinstone S. M., Purcell R. H., Hoofnagle J. H., Barker L. F., London W. T., Popper H., Peterson J. M., Kapikian A. Z. 1975; Experimental infection of chimpanzees with hepatitis A virus. Journal of Infectious Diseases 132:532–545
    [Google Scholar]
  13. Forss S. K., Strebel K., Beck E., Schaller H. 1984; Nucleotide sequence and genome organization of foot-and-mouth disease virus. Nucleic Acids Research 12:6587–6601
    [Google Scholar]
  14. Fucharoen S., Fucharoen G., Fucharoen P., Fukumaki Y. 1989; A novel ochre mutation in the beta-thalassemia gene a Thai. Journal of Biological Chemistry 264:7780–7783
    [Google Scholar]
  15. Gelfand D. H., White T. 1990; Thermostable DNA polymerase. In PCR ProtocolsA Guide to Method and Application pp. 129–141 Edited by Innis M. A. Gelfand D. H., Sninsky J. J., White T. J. New York & London: Academic Press;
    [Google Scholar]
  16. Goad W. B., Kanehisa M. I. 1982; Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries. Nucleic Acids Research 10:247–263
    [Google Scholar]
  17. Jansen R. W., Siegl G., Lemon S. M. 1990; Molecular epidemiology of human hepatitis A virus defined by an antigen-capture polymerase chain reaction method. Proceedings of the National Academy of Sciences, U,. S,. A 87:2867–2871
    [Google Scholar]
  18. Karetnyi Yu. V., Andzhaparidze A. G., Orlova T. M., Balayan M. S. 1989; Study of hepatitis virus isolates of human and simian origin by enzyme immunoassay using polyclonal and monoclonal antibodies. Voprosy Virusologii 1:50–53
    [Google Scholar]
  19. Kitamura N. B., Semler L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., Werf S. V. D., Andersen C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature, London 291:547–553
    [Google Scholar]
  20. LeDuc J. W., Lemon S. M., Keenan C. M., Graham R. R., Marchwicki R. H., Binn L. N. 1983; Experimental infection of a New World owl monkey (Aotus trivigatus) with hepatitis A virus. Infection and Immunity 40:766–772
    [Google Scholar]
  21. Lemon S. M., Binn L. N., Marchwicki R., Murphy P. C., Ping L.-H., Jansen R. W., Asher L. V. S., Stapleton J. T., Taylor D. G., LeDuc J. W. 1990; In vivo replication and reversion to wild type of a neutralization-resistant antigenic variant of hepatitis A virus. Journal of Infectious Diseases 161:7–13
    [Google Scholar]
  22. Linemeyer D. L., Menke J. G., Martin-Gallarado A., Hughes J. V., Young A., Mitra S. W. 1985; Molecular cloning and partial sequencing of hepatitis A viral cDNA. Journal of Virology 54:247–255
    [Google Scholar]
  23. MacGregor A., Kornitschuk M., Hurrell J. G. R., Lehman N. I., Coulepis A. G., Locarnini S. A., Gust I. D. 1983; Monoclonal antibodies against hepatitis A virus. Journal of Clinical Microbiology 18:1237–1243
    [Google Scholar]
  24. Mao J. S., Go Y. Y., Huang H. Y., Yu P. H., Huang B. Z., Ding Z. S., Chen N. L., Yu J. H., Xie R. Y. 1981; Susceptibility of monkeys to human hepatitis A. Journal of Infectious Diseases 144:55–60
    [Google Scholar]
  25. Najarian R. D., Caput D., Gee W., Potter S. J., Renard A., Merryweather J., Nest G. V., Dina D. 1985; Primary structure and gene organization of human hepatitis A virus. Proceedings of the National Academy of Sciences, U,. S,. A 82:2627–2631
    [Google Scholar]
  26. Palmenberg A. C., Kirby E. M., Janda R. R., Drake N. L., Duke G. M., Potratz K. F., Collett M. S. 1984; The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region. Nucleic Acids Research 12:2969–2985
    [Google Scholar]
  27. Paul A. V., Tada H., von der Helm K., Wissel T., Kiehn R., Wimmer E., Deinhardt F. 1987; The entire nucleotide sequence of the genome of human hepatitis A virus (isolate MBB). Virus Research 8:153–171
    [Google Scholar]
  28. Ping L.-H., Jansen R. W., Stapleton J. T., Cohen J. I., Lemon S. M. 1988; Identification of an immunodominant antigenic site involving the capsid protein VP3 of hepatitis A virus. Proceedings of the National Academy of Sciences, U,. S,. A 85:8281–8285
    [Google Scholar]
  29. Racaniello V. R., Baltimore D. 1981; Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proceedings of the National Academy of Sciences, U,. S,. A 78:4887–1891
    [Google Scholar]
  30. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Ehrlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  31. Skern T., Sommergruber W., Blaas D., Gruendler P., Fravadorfer F., Pieler C., Fogy I., Kuechler E. 1985; Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region. Nucleic Acids Research 13:2111–2126
    [Google Scholar]
  32. Stanway G. P., Hughes J., Mountford R. C., Minor P. D., Almond J. W. 1984; The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Research 12:7859–7875
    [Google Scholar]
  33. Sverdlov E. D., Tsarev S. A., Markova S. V., Vasilenko S. K., Chizhikov V. E., Petrov N. A., Kusov Yu. Yu., Nastashenko T. A., Balayan M. S. 1987; Cloning and expression of hepatitis A genome in E.coli cells. Molecular Genetics 6:129–133
    [Google Scholar]
  34. Ticehurst J. R., Feinstone S. M., Chestnut T., Tassopoulos N. C., Popper H., Purcell R. H. 1987; Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization. Journal of Clinical Microbiology 25:1822–1829
    [Google Scholar]
  35. Tindal K. R., Kunkel T. A. 1988; Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Biochemistry 27:6008–6013
    [Google Scholar]
  36. Winship P. R. 1989; An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Research 17:1266
    [Google Scholar]
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