1887

Abstract

Two mutant strains of beet necrotic yellow vein virus (BNYVV) containing deletion mutants of RNA-2 were produced during serial passage in mechanically inoculated leaves. The mutant strains were referred to as S-0a (RNA-1+2a) and G-0b (RNA-1+2b). RNA-2a and RNA-2b were about 4.3 kb and 4.2 kb in length, respectively, whereas normal sized RNA-2 was about 4.8 kb in length. translation and immunoblot analysis showed that RNA-2, RNA-2a and RNA-2b all directed synthesis of the coat protein ( 22K). However, whereas wild-type RNA-2 also directed the synthesis of a coat protein read-through protein with an of 83K (predicted 75K), RNA-2a and RNA-2b directed the production of readthrough proteins with s of 67K and 58K, respectively. This suggests that the deleted regions of RNA-2a and RNA-2b occur within the second open reading frame, which encodes a polypeptide of 54K, which is translated by readthrough of the coat protein cistron. After the addition of wild-type RNA-3 and RNA-4 to all the strains, the mutant strains could not be transmitted by zoospores produced from either zoosporangia or resting spores, whereas the wild-type strains were readily transmitted. These results indicate that the 75K readthrough protein encoded by RNA-2 is essential for the transmission of BNYVV by .

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/content/journal/jgv/10.1099/0022-1317-72-7-1497
1991-07-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-7-1497
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