Human herpesvirus 6 (HHV-6) DNA was detected in peripheral blood from exanthem subitum patients during the acute and convalescent phases of infection using the polymerase chain reaction. Although DNA could be detected in non-adherent and adherent mononuclear cells during the acute phase, it was detected predominantly in adherent cells during the convalescent phase; furthermore, viral DNA was found in adherent cells of healthy adults. When adherent mononuclear cells were cultured in vitro, virus was found to replicate well in differentiated cells cultured for 7 days in vitro before infection. When cells were cultured for more than 1 month, no detectable antigen and no evidence of virus growth was observed, but viral DNA could be detected. These apparently latently infected monocytes were treated with phorbol ester, after which virus could be recovered from the cultures. Therefore, we have developed an in vitro latency system for HHV-6; our results suggest that HHV-6 may latently infect monocytes in vivo and in vitro and that it may be reactivated in cells by some factors.
AgutH.,
GuetardD.,
CollandreH.,
DauguetC.,
MontagnierL.,
MicleaJ. M.,
BaurmannH.,
GessainA.1988; Concomitant infection by human herpesvirus 6, HTLV-1 and HIV-2. Lancet i:712
ArbeitR. D.,
ZaiaJ. A.,
ValerioM. A.,
LevinM. J.1982; Infection of human peripheral blood mononuclear cells by varicella-zoster virus. Intervirology 18:56–65
AsadaH.,
YalsinS.,
BalachandraK.,
HigashiK.,
YamanishiK.1989; Establishment of a titration system for human herpesvirus 6 and evaluation of neutralizing antibody response to the virus. Journal of Clinical Microbiology 27:2204–2207
BuchbinderA.,
JosephsS. F.,
AblashiD.,
SalahuddinS. Z.,
KlotmanM. E.,
ManakM.,
KruegerG. R. F.,
Wong-StaalF.,
GalloR. C.1988; Polymerase chain reaction amplification and in situ hybridization for the detection of human B lymphotropic virus. Journal of Virotogical Methods 21:191–197
DanielsC. A.,
KleinermanE. S.,
SnydermanR.1978; Abortive and productive infections of human mononuclear phagocytes by type I herpes simplex virus. American Journal of Pathology 91:119–136
Domke-OpitzI.,
StraubP.,
KirchnerJ.1986; Effect of interferon on replication of herpes simplex virus type 1 and 2 in human macrophages. Journal of Virology 60:37–42
EfstathiouS.,
GompelsU. A.,
CraxtonM. A.,
HonessR. W.,
WardK.1988; DNA homology between a novel human herpesvirus (HHV-6) and human cytomegalovirus. Lancet i:63–64
EnsoliB.,
LussoP.,
SchachterF.,
JosephsS. F.,
RappaportJ.,
NegroF.,
GalloR. C.,
Wong-StaalF.1989; Human herpesvirus-6 increases HIV-1 expression in co-infected T cells via nuclear factors binding to the HIV-1 enhancer. EMBO Journal 8:3019–3027
HammerstromJ.1979; Human macrophage differentiation in vivo and in vitro; a comparison of human macrophages and monocytes. Acta pathologica et microbiologica scandinavica 87:113–120
HorvatR. T.,
WoodC.,
BalachandranN.1989; Transactivation of human immunodeficiency virus promoter by human herpesvirus 6. Journal of Virology 63:970–973
KondoK.,
HayakawaY.,
MoriH.,
SatoS.,
KondoT.,
TakahashiK.,
MinamishimaY.,
TakahashiM.,
YamanishiK.1990; Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum. Journal of Clinical Microbiology 28:970–974
LawrenceG. L.,
CheeM.,
CraxtonM. A.,
GompelsU. A.,
HonessR. W.,
BarrelB. G.1990; Human herpesvirus 6 is closely related to human cytomegalovirus. Journal of Virology 64:287–299
LussoP.,
MarkhamP. D.,
IschachlerE.,
VeroneseF. M.,
SalahuddinS. Z.,
AblashiD. V.,
PahwaS.,
KrohnK.,
GalloR. C.1988; In vitro cellular tropism of human B-lymphotropic virus (human herpesvirus-6). Journal of Experimental Medicine 167:1659–1670
LussoP.,
EnsoliB.,
MarkhamP. D.,
AblashiD. V.,
SalahuddinS. Z.,
TschachlerE.,
Wong-StaalF.,
GalloR. C.1989; Productive dual infection of human CD4+ T lymphocytes by HIV-1 and HHV-6. Nature, London 337:370–373
OkunoT.,
TakahashiK.,
BalachandraK.,
ShirakiK.,
YamanishiK.,
TakahashiM.,
BabaK.1989; Seroepidemiology of human herpesvirus 6 infection in normal children and adults. Journal of Clinical Microbiology 27:651–653
OkunoT.,
SaoH.,
AsadaH.,
ShirakiK.,
TakahashiM.,
YamanishiK.1990; Analysis of a glycoprotein of human herpesvirus 6 (HHV-6) using monoclonal antibodies. Virology 176:625–628
PackardB. S.,
TavassoliM.,
DaleG. L.,
BeutlerE.1982; A method for the establishment and long-term maintenance of in vitro monocytic culture with functional and morphological homogenicity. Blood 60:623–626
PietroboniG. H.,
HarnettG. B.,
FarrT. J.,
BucensM. R.1988; Human herpesvirus type 6 (HHV-6) and its in vitro effect on human immunodeficiency virus (HIV). Journal of Clinical Pathology 41:1310–1312
SaikiR. K.,
ScharfS.,
FaloonaF.,
MullisK. B.,
HornG. T.,
ErlichH. A.,
ArnheimN.1985; Enzymic amplification of /β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
SaikiR. K.,
GelfondD. H.,
StoffelS.,
ScharfS. J.,
HiguchiR.,
HornG. T.,
MullisK. M.,
ErlichH. A.1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
SalahuddinS. Z.,
AblashiD. V.,
MarkhamP. D.,
JosephsS. F.,
SturzeneggerS.,
KaplanM.,
HalliganG.,
BiberfeldP.,
StaalS. W.,
KramarskyB.,
GalloR. C.1986; Isolation of a new-virus, HBLV, in patients with lymphoproliferative disorders. Science 234:596–600
YamanishiK.,
OkunoT.,
ShirakiK.,
TakahashiM.,
KondoT.,
AsanoY.,
KurataT.1988; Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet i:1065–1067
ZuckermanS. H.,
AckermanS. K.,
DouglasS. D.1979; Longterm human peripheral blood monocyte cultures: establishment, metabolism and morphology of primary human monocyte-macrophage cell culture. Immunology 38:401–411