%0 Journal Article %A Pfleiderer, Michael %A Routledge, Edward %A Herrler, Georg %A Siddell, Stuart G. %T High level transient expression of the murine coronavirus haemagglutinin-esterase %D 1991 %J Journal of General Virology, %V 72 %N 6 %P 1309-1315 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-72-6-1309 %I Microbiology Society, %X We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemad-sorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-6-1309