@article{mbs:/content/journal/jgv/10.1099/0022-1317-72-6-1309, author = "Pfleiderer, Michael and Routledge, Edward and Herrler, Georg and Siddell, Stuart G.", title = "High level transient expression of the murine coronavirus haemagglutinin-esterase", journal= "Journal of General Virology", year = "1991", volume = "72", number = "6", pages = "1309-1315", doi = "https://doi.org/10.1099/0022-1317-72-6-1309", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-6-1309", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemad-sorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.", }