1887

Journal of General Virology header logo

Volume 72, Issue 6

Characterization of human antibody-binding sites on the external envelope of human immunodeficiency virus type 2 Free

Abstract

Antibody-reactive peptide scanning (Pepscan) using overlapping nonapeptides and human sera as probes allows the identification of amino acids contributing significantly to antigen-antibody interaction. Five-hundred and two overlapping nonapeptides derived from the human immunodeficiency virus type 2 strain Rod (HIV-2) external envelope glycoprotein gp125 were synthesized to serve as probes for reactivity with eight sera of HIV-2-infected individuals. Fifteen antibody-binding regions were identified, among which two amino-terminal regions [E3, amino acids (aa) 118 to 132; E4, aa 125 to 141] and four carboxy-terminal regions (E11, aa 303 to 324; E12, aa 340 to 358; E14, aa 436 to 452; E15, aa 486 to 507) were the most antigenic. The amino acids in binding sites E3 and E4 were highly variable among simian immunodeficiency virus (SIV), HIV-2 and HIV-1. The antibody-binding domains E14 and E15 were highly conserved among HIV-2 strains (94% and 86% identity of HIV-2 to HIV-2 and HIV-2, respectively). Both domains had more amino acids in common with SIV (88% for E14, 64% for E15) than with HIV-1 (41% for E14, 45% for E15). Epidemiological studies revealed that the sera of African HIV-2-infected individuals bound the E11 and E15 peptides best (31%, 8/26). The sera of African HIV-1-infected individuals showed significant levels of cross-reactivity to the HIV-2 peptides, especially to the E15 peptide, whereas the sera of European HIV-1-infected individuals showed only moderate levels of cross-reactivity. If peptides covering the E15 epitope were used, African HIV-2-positive sera showed only a low level of cross-reactivity (4%) to E15 of HIV-1 and SIV. African HIV-1-positive sera bound the HIV-1 E15 peptide best (81%, 52/64), but showed high levels of cross-reactivity to SIV E15 (17%, 11/64) and HIV-2 E15 (25%, 16/64). European HIV-1-positive sera showed a high level of reactivity to HIV-1 E15 (91%, 50/55) and a low level of cross-reactivity to the HIV-2 (2%, 1/55), and none to the SIV E15 (0/55) peptide. These results indicate that the immunodominant regions of the HIV-2 external envelope (E11 and E15) align with the most immunodominant regions of HIV-1.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1991
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-6-1261
1991-06-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/6/JV0720061261.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-6-1261&mimeType=html&fmt=ahah

References

  1. Barin F., M’Boup S., Denis F., Kanki P., Allan J. S., Lee T. H., Essex M. 1985; Serological evidence for virus related to simian T-lymphotropic retrovirus III in residents of West Africa. Lancet ii:1387–1389
     [Google Scholar]
  2. Böttiger B., Karlsson A., Nauclér A., Andreasson P.-A., Mendes Costa C., Biberfield G. 1989; Cross-neutralizing antibodies against HIV-1 (HTLV-IIIB and HTLV-IIIRF) and HIV-2 (SBL-6669 and a new isolate SBL-K135). Aids Research and Human Retrovirus 5:511–519
     [Google Scholar]
  3. Böttiger B., Karlsson A., Andreasson P.-A., Nauclér A., Mendes Costa C., Norrby E., Biberfield G. 1990; Envelope cross-reactivity between human immunodeficiency virus types 1 and 2 detected by different serological methods: correlation between cross-neutralization and reactivity against the main neutralizing site. Journal of Virology 64:3492–3499
     [Google Scholar]
  4. Chakrabarti L., Guyader M., Alizon M., Daniel M. D., Desrosiers R. C., Tiollais P., Sonigo P. 1987; Sequence of simian immunodeficiency virus from macaque and its relationship to other human and simian retroviruses. Nature, London 328:543–547
     [Google Scholar]
  5. Clavel F. 1987; HIV-2, the West African AIDS virus. AIDS 1:135–140
     [Google Scholar]
  6. Clavel F., Guetard D., Brun-Vezinet F., Chamaret S., Rey M. A., Santos-Ferreira M. O., Laurent A.-G., Dauguet C., Katlama C., Rouzioux C., Klatzmann D., Champalimaud J. L., Montagnier L. 1986; Isolation of a new human retrovirus from West African patients with AIDS. Science 233:343–346
     [Google Scholar]
  7. De Wolf F., Lange J. M. A., Houweling J. T. M., Coutinho R. A., Schellekens P. T., Van der Noordaa J., Goudsmit J. 1988; Numbers of CD4+ cells and the levels of core antigens of and antibodies to the human immunodeficiency virus as predictors of AIDS among seropositive homosexual men. Journal of Infectious Diseases 158:615–622
     [Google Scholar]
  8. Franchini G., Gurgo C., Guo H. G., Gallo R. C., Collalti E., Fargnoli K. A., Hall L. F., Wong-Staal F., Reitz M. S. Jr 1987; Sequence of simian immunodeficiency virus and its relationship to the human immunodeficiency viruses. Nature, London 328:539–542
     [Google Scholar]
  9. Geysen H. M., Meloen R. H., Barteling S. J. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences, U.S.A 81:3998–4002
     [Google Scholar]
  10. Geysen H. M., Barteling S. J., Meloen R. H. 1985; Small peptides induce antibodies with a sequence and structural requirement for binding antigen comparable to antibodies raised against the native protein. Proceedings of the National Academy of Sciences, U.S.A 82:178–182
     [Google Scholar]
  11. Goudsmit J. 1988; Immunodominant B-cell epitopes of the HIV-1 envelope recognized by infected and immunized hosts. AIDS 2: supplement 1S41–S45
     [Google Scholar]
  12. Goudsmit J., DeBouck C., Meloen R. H., Smit L., Barker M., Asher D. M., Wolff A. V., Gibbs C. J., Gajdusek D. C. 1988a; Human immunodeficiency virus type 1 neutralizing epitope with conserved architecture elicits early type-specific antibodies in experimentally infected chimpanzees. Proceedings of the National Academy of Sciences, U.S.A 85:4478–4482
     [Google Scholar]
  13. Goudsmit J., Boucher C. A. B., Meloen R. H., Epstein L. G., Smit L., Van Der Hoek L., Barker M. 1988b; Human antibody response to a strain-specific HIV-1 gpl20 epitope associated with cell fusion inhibition. AIDS 2:157–164
     [Google Scholar]
  14. Goudsmit J., Meloen R. H., Brasseur R., Barin F. 1989; Human B-cell epitopes of HIV-2 transmembrane protein are similarly spaced as in HIV-1. Journal of Acquired Immune Deficiency Syndromes 2:297–302
     [Google Scholar]
  15. Goudsmit J., Meloen R. H., Brasseur R. 1990; Map of sequential B cell epitopes of the HIV-1 transmembrane protein using human antibodies as probe. Intervirology 31:327–338
     [Google Scholar]
  16. Guyader M., Emerman M., Sonigo P., Clavel F., Montagnier L., Alizon M. 1987; Genome organization and transactivation of the human immunodeficiency virus type 2. Nature, London 326:662–669
     [Google Scholar]
  17. Ho D. D., Sarngadharan M. G., Hirsch M. S., Schooley R. T., Rota T. R., Kennedy R. C., Chanh T. C., Sato V. L. 1987; Human immunodeficiency virus neutralizing antibodies recognize several conserved domains on the envelope glycoprotein. Journal of Virology 61:2024–2028
     [Google Scholar]
  18. Leonard C. K., Spellman M. W., Riddle L., Harris R. J., Thomas J. N., Gregory T. J. 1990; Assignment of interchain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gpl20) expressed in Chinese hamster ovary cells. Journal of Biological Chemistry 265:10373–10382
     [Google Scholar]
  19. Myers G., Rabson A. B., Berzofsky J. A., Smith T. F., WongStaal F. (editors) 1990 Human Retroviruses and AIDS Los Alamos: Los Alamos National Laboratory;
     [Google Scholar]
  20. Neurath A. R., Strick N., Lee E. S. Y. 1990a; B cell epitope mapping of human immunodeficiency virus envelope glycoproteins with long (19-to 36-residue) synthetic peptides. Journal of General Virology 71:85–95
     [Google Scholar]
  21. Neurath A. R., Strick N., Taylor P., Rubinstein P., Stevens C. E. 1990b; Search for epitope-specific antibody responses to the human immunodeficiency virus (HIV-1) envelope glycoproteins signifying resistance to disease development. AIDS Research and Human Retroviruses 6:1183–1192
     [Google Scholar]
  22. Norrby E., Biberfeld G., Chioidi F., Von Gegerfeldt A., Nauclér A., Parks E., Lerner R. 1987; Discrimination between antibodies to HIV and to related retroviruses using site-directed serology. Nature, London 329:248–250
     [Google Scholar]
  23. Palker T. J., Matthews T. J., Clark M. E., Cianciolo G. J., Randall R. R., Langlois A. J., White G. C., Safai B., Snyderman R., Bolognesi D. P., Haynes B. F. 1987; A conserved region at the COOH terminus of the human immunodeficiency virus gpl20 envelope protein contains an immunodominant epitope. Proceedings of the National Academy of Sciences, U.S.A 84:2479–2483
     [Google Scholar]
  24. Rusche J. R., Javaherian K., McDanal C., Petro J., Lynn D. L., Grimaila R., Langlois A., Gallo R. C., Arthur L. O., Fischinger P. J., Bolognesi D. P., Putney S. D., Matthews T. J. 1988; Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gpl20. Proceedings of the National Academy of Sciences, U.S.A 85:3189–3202
     [Google Scholar]
  25. Tedder R. S., Hughes T., O’Connor T., N’jie H., Whittle H. 1988; Envelope cross-reactivity in Western blot for HIV-1 and HIV-2 may not indicate dual infection. Lancet ii:927–930
     [Google Scholar]
  26. Van Tijn D. A., Boucher C. A. B., Barker M., Goudsmit J. 1989; Antigenicity of linear B-cell epitopes in the Cl, VI and V3 region of HIV-1 gpl20. Journal of Acquired Immune Deficiency Syndromes 2:303–306
     [Google Scholar]
  27. Weiss R. A., Clapham P. R., Weber J. N., Whitby D., Tedder R. S., O’Connor T., Chamaret S., Montagnier L. 1988; HIV-2 antisera cross-neutralize HIV-1. AIDS 2:95–100
     [Google Scholar]
  28. Wong-Staal F., Gallo R. C. 1985; Human T-lymphotropic retroviruses. Nature, London 317:395–403
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-6-1261
Loading
Characterization of human antibody-binding sites on the external envelope of human immunodeficiency virus type 2
J. Gen. Virol. 72, 1261 (1991); https://doi.org/10.1099/0022-1317-72-6-1261
/content/journal/jgv/10.1099/0022-1317-72-6-1261
/content/journal/jgv/10.1099/0022-1317-72-6-1261
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error