1887

Abstract

The human immunodeficiency virus type 1 (HIV-1) Gag protein was expressed in A549 cells infected with recombinant adenovirus types 4 and 7, each carrying the HIV-1 and genes. The Gag protein was assembled into enveloped virus-like particles that budded from plasma and vacuolar membranes. The particles, isolated by precipitation and isopycnic density centrifugation, contained both processed and unprocessed Gag-associated proteins.

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1991-06-01
2024-05-23
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References

  1. Allison A. C., Gregoriadis G. 1974; Liposomes as immunological adjuvants. Nature, London 252:252
    [Google Scholar]
  2. Ashorn P., McQuade T. J., Thaisrivongs S., Tomaselli A. G., Tarpley W. G., Moss B. 1990; An inhibitor of the protease blocks maturation of human and simian immunodeficiency viruses and spread of infection. Proceedings of the National Academy of Sciences, U,. S,. A 87:7472–7476
    [Google Scholar]
  3. Bryant M., Ratner L. 1990; Myristoylation-dependent replication and assembly of human immunodeficiency virus 1. Proceedings of the National Academy of Sciences, U,. S,. A 87:523–527
    [Google Scholar]
  4. Chanda P. K., Natuk R. J., Mason B. B., Bhat B. M., Greenberg L., Dheer S. K., Molnar-Kimber K. L., Mizutani S., Lubeck M. D., Davis A. R., Hung P. P. 1990; High level expression of the envelope glycoproteins of the human immunodeficiency virus type 1 in presence of rev gene using helper-independent adenovirus type 7 recombinants. Virology 175:535–547
    [Google Scholar]
  5. Clavel F., Orenstein J. M. 1990; A mutant of human immunodeficiency virus with reduced RNA packaging and abnormal particle morphology. Journal of Virology 64:5230–5234
    [Google Scholar]
  6. Debouck C., Gorniak J. G., Strickler J. E., Meek T. D., Metcalf B. W., Rosenberg M. 1987; Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor. Proceedings of the National Academy of Sciences, U,. S,. A. 84:8903–8906
    [Google Scholar]
  7. Edmondson W. P., Purcell R. H., Gundelfinger B. F., Love J. W. P., Ludwig W., Chanock R. M. 1966; Immunization by selective infection with type 4 adenovirus grown in human diploid tissue culture. I. Specific protective effect against epidemic disease. Journal of the American Medical Association 195:453–459
    [Google Scholar]
  8. Farmerie W. G., Loeb D. D., Casavant N. C., Hutchison C. A. III, Edgell M. H., Swanstrom R. 1987; Expression and processing of the AIDS virus reverse transcriptase in Escherichia coli . Science 236:305–308
    [Google Scholar]
  9. Feinberg M. B., Jarrett R. F., Aldovini A., Gallo R. C., Wong-Staal F. 1986; HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA. Cell 46:807–817
    [Google Scholar]
  10. Flexner C., Broyles S. S., Earl P., Chakrabarti S., Moss B. 1988; Characterization of human immunodeficiency virus gag/pol gene products expressed by recombinant vaccinia viruses. Virology 166:339–349
    [Google Scholar]
  11. Ford T. C., Rickwood D. 1982; Formation of isotonic Nycodenz gradients for cell separation. Analytical Biochemistry 124:293–298
    [Google Scholar]
  12. Gelderblom H. R., Hausmann E. H. S., Özel M., Pauli G., Koch M. A. 1987a; Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins. Virology 156:171–176
    [Google Scholar]
  13. Gelderblom H., Reupke H., Winkel T., Kunze R., Pauli G. 1987b; MHC-antigens: constituents of the envelopes of human and simian immunodeficiency viruses. Zeitschrift für Naturforschung 42c:1328–1334
    [Google Scholar]
  14. Gelderblom H. R., Özel M., Pauli G. 1989; Morphogenesis and morphology of HIV. Structure-function relations. Archives of Virology 106:1–13
    [Google Scholar]
  15. Gheysen D., Jacobs E., De Foresta F., Thiriart C., Francotte M., Thines D., De Wilde M. 1989; Assembly and release of HIV-1 precursor Pr55 gag virus-like particles from recombinant baculovirus-infected insect cells. Cell 59:103–112
    [Google Scholar]
  16. Goto T., Ikuta K., Zhang J. J., Morita C., Sano K., Komatsu M., Fujita H., Kato S., Nakai M. 1990; The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1. Archives of Virology 111:87–101
    [Google Scholar]
  17. Göttlinger H. G., Sodroski J. G., Haseltine W. A. 1989; Role of capsid precursor processing and myristoylation in morphogenesis and infectivity of human immunodeficiency virus type 1. Proceedings of the National Academy of Sciences, U,. S,. A 86:5781–5785
    [Google Scholar]
  18. Gowda S. D., Stein B. S., Engleman E. G. 1989; Identification of protein intermediates in the processing of the p55 HIV-1 gag precursor in cells infected with recombinant vaccinia virus. Journal of Biological Chemistry 264:8459–8462
    [Google Scholar]
  19. Gutekunst R., White R., Edmondson W. 1967; Immunization with live type 4 adenovirus: determination of infectious virus dose and protective effect of enteric infection. American Journal of Epidemiology 86:341–349
    [Google Scholar]
  20. Haffar O., Garrigues J., Travis B., Moran P., Zarling J., Hu S.-L. 1990; Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system. Journal of Virology 64:2653–2659
    [Google Scholar]
  21. Henderson L. E., Sowder R., Copeland T. D., Oroszlan S., Arthur L. O., Robey W. G., Fischinger P. J. 1987; Direct identification of class II histocompatibility DR proteins in preparations of human T-cell lymphotrophic virus type III. Journal of Virology 61:629–632
    [Google Scholar]
  22. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  23. Hockley D. J., Wood R. D., Jacobs J. P., Garrett A. J. 1988; Electron microscopy of human immunodeficiency virus. Journal of General Virology 69:2455–2469
    [Google Scholar]
  24. Hu S.-H., Travis B. M., Garrigues J., Zarling J. M., Sridhar P., Dykers T., Eichberg J. W., Alpers C. 1990; Processing, assembly, and immunogenicity of human immunodeficiency virus core antigens expressed by recombinant vaccinia virus. Virology 179:321–329
    [Google Scholar]
  25. Jacks T., Power M. D., Masiarz F. R., Luciw P. A., Barr P. J., Varmus H. E. 1988; Characterization of ribosomal frameshifting in HIV-1 gag-pol expression. Nature, London 331:280–283
    [Google Scholar]
  26. Jacobs E., Gheysen D., Thines D., Francotte M., De Wilde M. 1989; The HIV-1 Gag precursor Pr55 gag synthesized in yeast is myristoylated and targeted to the plasma membrane. Gene 79:71–81
    [Google Scholar]
  27. Karacostas V., Nagashima K., Gonda M. A., Moss B. 1989; Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector. Proceedings of the National Academy of Sciences, U,. S,. A 86:8964–8967
    [Google Scholar]
  28. Katsumoto T., Hattori N., Kurimura T. 1987; Maturation of human immunodeficiency virus, strain LAV, in vitro. Intervirology 27:148–153
    [Google Scholar]
  29. Kohl N. E., Emini E. A., Schleif W. A., Davis L. J., Heimbach J. C., Dixon R. A. F., Scolnick E. M., Sigal I. S. 1988; Active human immunodeficiency virus protease is required for viral infectivity. Proceedings of the National Academy of Sciences, U,. S,. A 85:4686–4690
    [Google Scholar]
  30. Kramer R. A., Schaber M. D., Skalka A. M., Ganguly K., Wong-Staal F., Reddy E. P. 1986; HTLV-III gag protein is processed in yeast cells by the virus pol- protease. Science 231:1580–1584
    [Google Scholar]
  31. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  32. Lawrence W. C. 1976; Purification of equine herpesvirus type 1. Journal of General Virology 31:81–91
    [Google Scholar]
  33. Mervis R. J., Ahmad N., Lillehoj E. P., Raum M. G., Salazar F. H. R., Chan H. W., Venkatesan S. 1988; The gag gene products of human immunodeficiency virus type 1: alignment within the gag open reading frame, identification of posttranslational modifications, and evidence for alternative gag precursors. Journal of Virology 62:3993–4002
    [Google Scholar]
  34. Morein B., Sundquist B., Höglund S., Dalsgaard K., Osterhaus A. 1984; Iscom, a novel structure for antigenic presentation of membrane proteins from enveloped viruses. Nature, London 308:457–460
    [Google Scholar]
  35. Munn R. J., Marx P. A., Yamamoto J. K., Gardner M. B. 1985; Ultrastructural comparison of the retroviruses associated with human and simian acquired immunodeficiency syndromes. Laboratory Investigation 53:194–199
    [Google Scholar]
  36. Overton H. A., McMillan D. J., Gridley S. J., Brenner J., Redshaw S., Mills J. S. 1990; Effect of two novel inhibitors of the human immunodeficiency virus protease on the maturation of the HIV gag and gag-pol polyproteins. Virology 179:508–511
    [Google Scholar]
  37. Özel M., Pauli G., Gelderblom H. R. 1988; The organization of the envelope projections on the surface of HIV. Archives of Virology 100:255–266
    [Google Scholar]
  38. Palmer E., Sporborg C., Harrison A., Martin M. L., Feorino P. 1985; Morphology and immunoelectron microscopy of AIDS virus. Archives of Virology 85:189–196
    [Google Scholar]
  39. Pekovic D., Garzon S., Strykowski H., Ajdukovick D., Gornitsky M., Dupuy J.-M. 1986; Immunogold labeling of HTLV-III/LAV in H9 cells studied by transmission electron microscopy. Journal of Virological Methods 13:265–269
    [Google Scholar]
  40. Peng C., Ho B. K., Chang T. W., Chang N. T. 1989; Role of immunodeficiency virus type 1-specific protease in core protein maturation and viral infectivity. Journal of Virology 63:2550–2556
    [Google Scholar]
  41. Polson A., Stannard L. 1970; Concentration of viruses at low rotor velocities. Virology 40:781–791
    [Google Scholar]
  42. Ratner L., Haseltine W., Patarca R., Livak K. J., Starcich B., Josephs S. F., Doran E. R., Rafalski J. A., Whitehorn E. A., Baumeister K., Ivanoff L., Pettewav S. R. Jr, Pearson M. L., Lautenberger J. A., Papas T. S., Ghrayeb J., Chang N. T., Gallo R. C., Wong-Staal F. 1985; Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature, London 313:277–284
    [Google Scholar]
  43. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  44. Rhee S. S., Hunter E. 1990; Structural role of the matrix protein of type D retroviruses in Gag polyprotein stability and capsid assembly. Journal of Virology 64:4383–4389
    [Google Scholar]
  45. Rickwood D., Ford T., Graham J. 1982; Nycodenz: a new nonionic iodinated gradient medium. Analytical Biochemistry 123:23–31
    [Google Scholar]
  46. Shioda T., Shibuta H. 1990; Production of human immunodeficiency virus (HIV)-like particles from cells infected with recombinant vaccinia viruses carrying the gag gene of HIV. Virology 175:139–148
    [Google Scholar]
  47. Simionescu N., Simionescu M. 1976; Galloylglucoses of low molecular weight as mordant in electron microscopy. I. Procedure, and evidence for mordanting effect. Journal of Cell Biology 70:608–621
    [Google Scholar]
  48. Smith A. J., Cho M.-L., Hammarskjöld M.-L., Rekosh D. 1990; Human immunodeficiency virus type 1 Pr55 gag and Prl60 gag-po1 expressed from a simian virus 40 late replacement vector are efficiently processed and assembled into virus-like particles. Journal of Virology 64:2743–2750
    [Google Scholar]
  49. Sodroski J., Goh W. C., Rosen C., Dayton A., Terwilliger E., Haseltine W. 1986; A second post-transcriptional trans-activator gene required for HTLV-III replication. Nature, London 321:412–417
    [Google Scholar]
  50. Stewart L., Schatz G., Vogt V. M. 1990; Properties of avian retrovirus particles defective in viral protease. Journal of Virology 63:5076–5092
    [Google Scholar]
  51. Sussenbach J. S. 1984; The structure of the genome. In The Adenoviruses pp 35–124 Edited by Ginsberg H. S. New York & London: Plenum Press;
    [Google Scholar]
  52. Top F. H. Jr, Buescher E. L., Bancroft W. H., Russell P. K. 1971; Immunization with live types 7 and 4 adenovirus vaccines. II. Antibody response and protective effect against acute respiratory disease due to adenovirus type 7. Journal of Infectious Diseases 124:155–160
    [Google Scholar]
  53. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U,. S,. A 76:4350–4354
    [Google Scholar]
  54. Veronese F. D., Copeland T. D., Oroszlan S., Gallo R. C., Sarngadharan M. G. 1988; Biochemical and immunological analysis of human immunodeficiency virus gag gene products pl7 and p24. Journal of Virology 62:795–801
    [Google Scholar]
  55. Wain-Hobson S., Sonigo P., Danos O., Cole S., Alizon M. 1985; Nucleotide sequence of the AIDS virus, LAV. Cell 40:9–17
    [Google Scholar]
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