1887

Abstract

In nature, rice tungro disease is caused by an RNA and a DNA virus complex, but we have obtained an independently infectious clone of rice tungro bacilliform virus (RTBV) DNA. Infectivity could be demonstrated only when a more than unit-length copy was cloned in the binary vector Bin19 and agroinoculated into rice plants. Rice plants thus agroinfected with cloned RTBV DNA showed typical symptoms of tungro disease, presence of viral DNA and bacilliform particles, and could be used as a source of virus to infect healthy plants by the green leafhopper (). The importance of this infectious clone in understanding the molecular biology of RTBV and the rice tungro disease is discussed.

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1991-06-01
2024-04-25
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References

  1. Al-Mallah M. K., Davey M. R., Cocking E. C. 1987; Enzymatic treatment of clover root hairs removes a barrier to Rhizobium-host specificity. Bio/Technology 5:1319–1322
    [Google Scholar]
  2. Barbara D. J., Clark M. F. 1982; A simple indirect ELISA using F(ab′)2 fragments of immunoglobulin. Journal of General Virology 58:315–322
    [Google Scholar]
  3. Bevan M. 1984; Binary Agrobacterium vectors for plant transformation. Nucleic Adds Research 12:8711–8721
    [Google Scholar]
  4. Boulton M. I., Buchholz W. G., Marks M. S., Markham P. G., Davies J. W. 1989; Specificity of Agrobacterium- mediated delivery of maize streak virus DNA to members of Gramineae. Plant Molecular Biology 12:31–40
    [Google Scholar]
  5. Cabauatan P. Q., Hibino H. 1985; Transmission of rice tungro bacilliform and spherical viruses by Nephotettix virescens distant. Philippine Phytopathology 21:103–109
    [Google Scholar]
  6. Cabauatan P. Q., Hibino H. 1988; Isolation, purification and serology of rice tungro bacilliform and rice tungro spherical viruses. Plant Disease 72:526–528
    [Google Scholar]
  7. Dahal G., Hibino H., Saxena R. C. 1990; Association of leafhopper feeding behavior with transmission of rice tungro to susceptible and resistant rice cultivars. Phytopathology 80:371–377
    [Google Scholar]
  8. Ditta G., Stansfield S., Corbin D., Helinski D. R. 1980; Broad host range DNA cloning system for gram negative bacteria: construction of a gene bank of Rhizobium meliloti . Proceedings of the National Academy of Sciences, U,. S,. A. 77:7347–7351
    [Google Scholar]
  9. Donson J., Gunn H. V., Woolston C. J., Pinner M. S., Boulton M. I., Mullineux P. M., Davies J. W. 1988; Agrobacterium- mediated infectivity of digitaria streak virus DNA. Virology 162:248–250
    [Google Scholar]
  10. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  11. Galvez G. E. 1968; Purification and characterization of rice tungro virus by analytical density gradient centrifugation. Virology 35:418–426
    [Google Scholar]
  12. Grimsley N., Bisaro D. 1987; Agroinfection. In Plant DNA Infectious Agents pp 87–107 Edited by Hohn T., Schell J. Wien & New York: Springer-Verlag;
    [Google Scholar]
  13. Grimsley N., Hohn B., Hohn T., Walden R. 1986; ‘Agroinfection’, an alternative route for viral infection of plants by using the Ti plasmid. Proceedings of the National Academy of Sciences, U.S.A 83:3282–3286
    [Google Scholar]
  14. Grimsley N., Hohn T., Davies J. W., Hohn B. 1987; Agrobacterium- mediated delivery of infectious maize streak virus into maize plants. Nature. London 325:177–179
    [Google Scholar]
  15. Guilley H., Richards K. E., Jonard G. 1983; Observations concerning the discontinuous DNAs of cauliflower mosaic virus. EMBO Journal 2:277–282
    [Google Scholar]
  16. Hibino H., Cabauatan P. Q. 1987; Infectivity neutralization of rice tungro-associated viruses acquired by vector leafhoppers. Phytopathology 77:473–476
    [Google Scholar]
  17. Hibino H., Roechan M., Sudarisman S. 1978; Association of two types of virus particles with Penyakit Habang (tungro disease) of rice in Indonesia. Phytopathology 68:1412–1416
    [Google Scholar]
  18. Hibino H., Saleh N., Roechan M. 1979; Transmission of two kinds of rice tungro-associated viruses by insect vectors. Phytopathology 69:1266–1268
    [Google Scholar]
  19. Howell S. H., Walker L. L., Dudley R. K. 1980; Cloned cauliflower mosaic virus DNA infects turnips. (Brassica rapa) Science 208:1265–1267
    [Google Scholar]
  20. Hull R., Covey S. N. 1983; Does cauliflower mosaic virus replicate by reverse transcription?. Trends in Biochemical Sciences 8:119–121
    [Google Scholar]
  21. Jones M. C., Gough K., Dasgupta I., Subba Rao B. L., Cliffe J., Qu R., Shen P., Kaniewska M., Blakebrough M., Davies J. W., Beachy R. N., Hull R. 1991; Rice tungro disease is caused by an RNA and a DNA virus. Journal of General Virology 72:757–761
    [Google Scholar]
  22. Klapwijk P. M., Van Beelen P., Schilperoort R. A. 1979; Isolation of a recombination deficient Agrobacterium tumefaciens mutant. Molecular and General Genetics 173:171–175
    [Google Scholar]
  23. Klein T. M., Wolf E. D., Wu R., Sanford J. C. 1987; High-velocity microprojectiles for delivering nucleic acids into living cells. Nature, London 327:70–73
    [Google Scholar]
  24. Lebeurier G., Hirth L., Hohn T., Hohn B. 1980; Infectivities of native and cloned DNA of cauliflower mosaic virus. Gene 12:139–146
    [Google Scholar]
  25. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
    [Google Scholar]
  26. Omura T., Saito Y., Usugi T., Hibino H. 1983; Purification and serology of rice tungro spherical and rice tungro bacilliform viruses. Annals of the Phytopathological Society of Japan 49:73–76
    [Google Scholar]
  27. Ooms G., Karp A., Burrell M. M., Twell D., Roberts J. 1985; Genetic modification of potato development using Ri T-DNA. Theoretical and Applied Genetics 70:440–446
    [Google Scholar]
  28. Pfeiffer P., Hohn T. 1983; Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. Cell 33:781–789
    [Google Scholar]
  29. Raineri D. M., Bottino P., Gordon M. P., Nester E. W. 1990; Agrobacterium- mediated transformation of rice (Oryza sativa L.). Bio/Technology 8:33–38
    [Google Scholar]
  30. Roberts I. M., Harrison B. D. 1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
    [Google Scholar]
  31. Saito Y., Hibino H., Omura T., Inoue H. 1986; Virus diseases of rice: rice tungro virus. In Technical Bulletin of the Tropical Agriculture Research Centre, Japan21 Edited by Kajiwara T., Kuno S. Tropical Agricultural Research Centre, Ministry of Agriculture, Forests and Fisheries; Japan:
    [Google Scholar]
  32. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Woolston C. J., Barker R., Gunn H., Boulton M. I., Mullineux P. 1988; Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA. Plant Molecular Biology 11:35–43
    [Google Scholar]
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