1887

Abstract

A small RNA species with the structural and functional properties characteristic of viroids has been isolated from three different pear sources each of which induced symptoms of the pear blister canker (PBC) disease when indexed in the pear indicator A 20. A close association between this RNA and PBC disease was established, since two of the three studied sources were known to be affected by this malady only, and the viroid was not detected in healthy pear tissue. Moreover, the PBC-associated viroid (PBCVd) replicated when purified preparations were inoculated into cucumber and pear plants. PBCVd behaved in denaturing polyacrylamide gels as a circular RNA with a molecular size of approximately 315 nucleotide residues. Analysis by dot blot hybridization indicated that PBCVd shares similarities in sequence with peach latent mosaic viroid and hop stunt viroid, and to a lesser extent with apple scar skin viroid.

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1991-06-01
2024-04-20
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References

  1. Bellamy A. R., Ralph R. K. 1968; Recovery and purification of nucleic acids by means of cetyltrimethylammonium bromide. Methods in Enzymology 12B:156–160
    [Google Scholar]
  2. Cordy C. B., MacSwan J. C. 1961; Some evidence that pear bark measles is seed-borne. Plant Disease Reporter 45:891
    [Google Scholar]
  3. Cropley R. 1960; Pear blister canker: a virus disease. Annual Report East Mailing Research Station for 1959 43:104
    [Google Scholar]
  4. Feng Q., Tien P. 1990; Pear – a natural host of apple scar skin viroid (ASSV). In VIIIth International Congress of Virology Abstracts, p 383 Berlin:
    [Google Scholar]
  5. Flores R. 1986; Detection of citrus exocortis viroid in crude extracts by dot-blot hybridization: conditions for reducing spurious hybridization results and for enhancing the sensitivity of the technique. Journal of Virological Methods 13:161–169
    [Google Scholar]
  6. Flores R., Duran-Vila N., Pallas V., Semancik J. S. 1985; Detection of viroid and viroid-like RNAs from grapevine. Journal of General Virology 66:2095–2102
    [Google Scholar]
  7. Flores R., Hernandez C., Desvignes J. C., Llacer G. 1990; Some properties of the viroid inducing the peach latent mosaic disease. Research in Virology 141:109–118
    [Google Scholar]
  8. Franklin R. M. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proceedings of the National Academy of Sciences, U.S.A 55:1504–1511
    [Google Scholar]
  9. Hadidi A., Yang X. 1990; Detection of pome fruit viroids by enzymatic cDNA amplification. Journal of Virological Methods 30:261–270
    [Google Scholar]
  10. Hadidi A., Huang C., Hammond R. W., Hashimoto J. 1990; Homology of the agent associated with dapple apple disease to apple scar skin viroid and molecular detection of these viroids. Phytopathology 80:263–268
    [Google Scholar]
  11. Hashimoto J., Koganezawa H. 1987; Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Research 19:7045–7052
    [Google Scholar]
  12. Igloi G. L. 1983; A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis. Analytical Biochemistry 134:184–188
    [Google Scholar]
  13. Kegler H. 1965; Bark split and decline in Beurre Hardy pear trees. Zastita Bilja 16:311–316
    [Google Scholar]
  14. Kegler H. 1967; Der viröse Bimenverfall, die Rindenrissigkeit und die Rindennekrose. Obstbau 7:21–23
    [Google Scholar]
  15. Kristensen H. R., Jorgensen H. A. 1957; Plantesygdomme i Danmark 1955. New attacks of virus diseases, fungi and bacteria. Pear bark split. Tidsskrift for Planteavl 61:617
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Mühlbach H. P., Faustmann O., Sänger H. L. 1983; Conditions for optimal growth of a PSTV-infected potato cell suspension and detection of viroid-complementary longer-than-unit-length RNA in these cells. Plant Molecular Biology 2:239–247
    [Google Scholar]
  18. Pallas V., Navarro A., Flores R. 1987; Isolation of a viroid-like RNA from hop different from hop stunt viroid. Journal of General Virology 68:3201–3205
    [Google Scholar]
  19. Sänger H. L., Ramm K., Domdey H., Gross H. J., Henco K., Riesner D. 1979; Conversion of circular viroid molecules to linear strands. FEBS Letters 99:117–122
    [Google Scholar]
  20. Sano T., Ohshima K., Hataya T., Uyeda I., Shikata E., Chou T.-G., Meshi T., Okada Y. 1986; A viroid resembling hop stunt viroid in grapevines from Europe, the United States and Japan. Journal of General Virology 67:1673–1678
    [Google Scholar]
  21. Sano T., Hataya T., Terai Y., Shikata E. 1989; Hop stunt viroid strains from dapple fruit disease of plum and peach in Japan. Journal of General Virology 70:1311–1319
    [Google Scholar]
  22. Schumacher J., Randles J. W., Riesner D. 1983; A two-dimensional electrophorectic technique for the detection of circular viroids and virusoids. Analytical Biochemistry 135:288–295
    [Google Scholar]
  23. Semancik J. S., Weathers L. G. 1972; Exocortis virus; an infectious free-nucleic-acid plant virus with unusual properties. Virology 47:456–466
    [Google Scholar]
  24. Shikata E. 1990; New viroids from Japan. Seminars in Virology 1:107–115
    [Google Scholar]
  25. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  26. Thomsen A. 1961; Split bark of pears (rough bark of pears). Tidsskrift for Planteavl 65:69–72
    [Google Scholar]
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