1887

Abstract

Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and the Osloss-c strains of bovine viral diarrhoea virus were produced and characterized by indirect immunofluorescence assay, radioimmuno-precipitation and neutralization tests. Fourteen MAbs directed against the NY-1 strain recognized the gp48 and showed a weak neutralizing activity in the presence of goat anti-mouse immunoglobulin serum.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-5-1195
1991-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/5/JV0720051195.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-5-1195&mimeType=html&fmt=ahah

References

  1. Bolin S., Moennig V., Kelso Gourley N. E., Ridpath J. 1988; Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Archives of Virology 99:117–123
    [Google Scholar]
  2. Collett M. S., Larson R., Belzer S. K., Retzel E. 1988; Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165:200–208
    [Google Scholar]
  3. Donis R. O., Corapi W., Dubovi E. J. 1988; Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56K to 58K glycoprotein. Journal of General Virology 69:77–86
    [Google Scholar]
  4. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using Protein A–Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  5. Goding J. W. 1980; Antibody production by hybridomas. Journal of Immunological Methods 39:285–308
    [Google Scholar]
  6. Greiser-Wilke I., Moennig V., Coulibaly C. O. Z., Dahle J., Leder L., Liess B. 1990; Identification of conserved epitopes on a hog cholera virus protein. Archives of Virology 111:213–225
    [Google Scholar]
  7. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Magar R., Minocha H. C., Lecomte J. 1988; Bovine viral diarrhea virus proteins; heterogeneity of cytopathogenic and non-cytopathogenic strains and evidence of a 53K glycoprotein neutralization epitope. Veterinary Microbiology 16:303–314
    [Google Scholar]
  10. Mignon B., Schwers A., Waxweiler S., Boulanger D., Dubuisson J., Brownlie J., Pastoret P.-P. 1990; Etude de la stabilite antigénique d’une souche non cytopathogene de virus BVD chez des animaux infectés expérimentalement de maniére persistante. Annales de Médecine Vétérinaire 134:325–329
    [Google Scholar]
  11. Seppälä I., Sarvas H., Peterfly F., Mäkelä O. 1981; The four subclasses of IgG can be isolated from mouse serum by using Protein A–Sepharose. Scandinavian Journal of Immunology 14:335–342
    [Google Scholar]
  12. Thiriart C., Collignon C., Sneyers M., Lambert A.-F., Franssen J.-D., Schwers A., Bruyns C., Urbain J., Pastoret P.-P. 1987; Génération d’anticorps monoclonaux neutralisant le rotavirus bovin pour des essais thérapeutiques chez le veau nouveauné infecté expérimentalement. Annales de Médecine Vétérinaire 131:275–286
    [Google Scholar]
  13. Weiland E., Thiel H.-J., Hess G., Weiland F. 1989; Development of monoclonal neutralizing antibodies against bovine viral diarrhea virus after pretreatment of mice with normal bovine cells and cyclophosphamide. Journal of Virological Methods 24:237–244
    [Google Scholar]
  14. Wensvoort G. 1989; Production and characterization of monoclonal antibodies against the ‘Chinese’ strain of hog cholera vaccine virus. In Epitopes on structural proteins of hog cholera (swine fever) virus pp 99–115 Ph.D. thesis, State University of Utrecht
    [Google Scholar]
  15. Xue W., Blecha F., Minocha H. C. 1990; Antigenic variations in bovine viral diarrhea viruses detected by monoclonal antibodies. Journal of Clinical Microbiology 28:1688–1693
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-5-1195
Loading
/content/journal/jgv/10.1099/0022-1317-72-5-1195
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error