1887

Abstract

Cultured human sensory neurons are directly susceptible to CVS rabies virus infection and produce virus yields of 10 p.f.u./ml; infection can persist for more than 20 days without any sign of c.p.e. The use of a compartmentalized two-chamber culture system, with access to either the cell soma or neuritic extensions, permitted the study of viral retrograde transport, which occurs at between 50 and 100 mm/day. Neurons of human origin were more susceptible to virus infection than rat neurons and the axonal transport of rabies virus was more efficient. Electron microscopy allowed virus transport and infection of human dorsal root ganglia neurons to be observed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-5-1191
1991-05-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/5/JV0720051191.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-5-1191&mimeType=html&fmt=ahah

References

  1. Ceccaldi P. E., Gillet J. P., Tsiang H. 1989; Inhibition of the transport of rabies virus in the central nervous system. Journal of Neuropathology and Experimental Neurology 48:620–630
    [Google Scholar]
  2. Ceccaldi P. E., Ermine A., Tsiang H. 1990; Continuous delivery of colchicine in the rat brain with osmotic pumps for inhibition of rabies virus transport. Journal of Virological Methods 28:79–84
    [Google Scholar]
  3. Charlton K. M., Casey G. A. 1979; Experimental rabies in skunks. Immunofluorescence, light and electron microscopic studies. Laboratory Investigation 41:36–44
    [Google Scholar]
  4. Coulon P., Derbin C., Kucera P., Lafay F., Prehaud C., Flamand A. 1989; Invasion of the peripheral nervous systems of adult mice by the CVS strain of rabies virus and its avirulent derivative AvOl. Journal of Virology 63:3550–3554
    [Google Scholar]
  5. Dotti C. G., Simons K. 1990; Polarized sorting of viral glycoproteins to the axon and dendrites of hippocampal neurons in culture. Cell 62:63–72
    [Google Scholar]
  6. Gillet J. P., Derer P., Tsiang H. 1986; Axonal transport of rabies virus in the central nervous system of the rat. Journal of Neuropathology and Experimental Neurology 45:619–634
    [Google Scholar]
  7. Iwasaki Y., Liu D. S., Yamamoto T., Konno H. 1985; On the replication and spread of rabies virus in the human central nervous system. Journal of Neuropathology and Experimental Neurology 44:185–195
    [Google Scholar]
  8. Kucera P., Dolivo M., Coulon P., Flamand A. 1985; Pathway of the early progression of virulent and avirulent rabies strains from the eye to the brain. Journal of Virology 55:158–162
    [Google Scholar]
  9. Löve A., Andersson T., Norrby E., Kristensson K. 1987; Mumps virus infection of dissociated rodent spinal ganglia in vitro. Expression and disappearance of viral structural proteins from neurons. Journal of General Virology 68:1755–1759
    [Google Scholar]
  10. Lycke E., Tsiang H. 1987; Rabies virus infection of cultured rat sensory neurons. Journal of Virology 61:2733–2741
    [Google Scholar]
  11. Lycke E., Hamark B., Johansson M., Krotochwill A., Lycke J., Svennerholm B. 1988; Herpes virus infection of the human sensory neuron. An electron microscopy study. Archives of Virology 101:87–104
    [Google Scholar]
  12. Matsumoto S. 1975; Electron microscopy of central nervous system infection. In The Natural History of Rabies pp 217–233 Edited by Baer G. M. New York: Academic Press;
    [Google Scholar]
  13. Murphy F. A. 1977; Rabies pathogenesis. Brief report. Archives of Virology 54:279–297
    [Google Scholar]
  14. Murphy F. A., Bauer S. P., Harrison A. K., Winn W. C. Jr 1973; Comparative pathogenesis of rabies-like viruses; viral infection and transit from inoculation site to the central nervous system. Laboratory Investigation 28:361–376
    [Google Scholar]
  15. Tsiang H. 1988; Interactions of rabies virus and host cells. In Rabies pp 67–100 Edited by Campbell J. B., Charlton K. M. Boston: Kluwer Academic;
    [Google Scholar]
  16. Tsiang H., Koulakoff A., Bizzini B., Berwald-Netter Y. 1983; Neurotropism of rabies virus. An in vitro study. Journal of Neuropathology and Experimental Neurology 42:439–452
    [Google Scholar]
  17. Tsiang H., DeLaporte S., Ambroise D. J., Derer M., Koenig J. 1986; Interaction of cultured rat myotubes and neurons from the spinal cord by rabies virus. Journal of Neuropathology and Experimental Neurology 45:28–42
    [Google Scholar]
  18. Tsiang H., Lycke E., Ceccaldi P. E., Ermine A., Hirardot X. 1989; The anterograde transport of rabies virus in rat sensory dorsal root ganglia neurons. Journal of General Virology 70:2075–2085
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-5-1191
Loading
/content/journal/jgv/10.1099/0022-1317-72-5-1191
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error