An orf virus sequence showing homology to the 14K ‘fusion’ protein of vaccinia virus Free

Abstract

The nucleotide sequence of a region of DNA 30 kb from the right end of the orf virus genome has been determined. Examination of the sequences revealed an open reading frame encoding a 10K peptide with significant amino acid homology to the 14K ‘fusion’ protein reported in vaccinia virus. The orf virus sequence has a 31% identity with the vaccinia virus protein, but a higher level of homology of core predicted residues. The secondary structure of both proteins is also similar. The occurrence of the TAAAT sequence upstream from the initiation codon indicates that the sequence is likely to be transcribed late in infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-5-1177
1991-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/5/JV0720051177.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-5-1177&mimeType=html&fmt=ahah

References

  1. Crampin J., Nicholson B. H., Robson B. 1978; Protein folding and heterogeneity inside globular proteins. Nature, London 272:558–560
    [Google Scholar]
  2. Dallo S., Rodriguez J. F., Esteban M. 1987; A 14K envelope protein of vaccinia virus with an important role in virus-host cell interaction is altered during virus persistence and determines the plaque size phenotype of the virus. Virology 159:423–432
    [Google Scholar]
  3. Davison A. J., Moss B. 1989; Structure of vaccinia virus late promoters. Journal of Molecular Biology 210:771–784
    [Google Scholar]
  4. Funahashi S., Sato T., Shida H. 1988; Cloning and characterization of the gene encoding the major protein of the A-type inclusion body of cowpox virus. Journal of General Virology 69:35–47
    [Google Scholar]
  5. Gershon P. D., Ansell D. M., Black D. N. 1989; A comparison of the genome organisation of capripoxvirus with that of the orthopoxviruses. Journal of Virology 63:4703–4708
    [Google Scholar]
  6. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  7. Lee-Chen G.-J., Niles E. G. 1988; Map positions of the 5′ ends of eight mRNAs synthesized from the late genes in the vaccinia virus Hin dIII D fragment. Virology 163:80–92
    [Google Scholar]
  8. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441
    [Google Scholar]
  9. McGeoch D. J. 1990; Protein sequence comparisons show that the pseudoproteases encoded by poxviruses and certain retroviruses belong to the deoxyuridine triphosphatase family. Nucleic Acids Research 18:4105–4110
    [Google Scholar]
  10. Mercer A. A., Fraser K., Barns G., Robinson A. J. 1987; The structure and cloning of orf virus DNA. Virology 157:1–12
    [Google Scholar]
  11. Mercer A. A., Fraser K., Stockwell P. A., Robinson A. J. 1989; A homology of retroviral pseudoproteases in the parapox-virus, orf virus. Virology 172:665–668
    [Google Scholar]
  12. Nicholson B. H. 1982; Correlation of sequence and spatial position in globular proteins. Biochemical Society Transactions 10:386–388
    [Google Scholar]
  13. Paez E., Dallo S., Esteban M. 1987; Virus attenuation and identification of structural proteins of vaccinia virus that are selectively modified during virus persistence. Journal of Virology 61:2642–2647
    [Google Scholar]
  14. Patel D. D., Pickup D. J. 1987; Messenger RNAs of a strongly-expressed late gene of cowpox virus contain 5′-terminal poly(A) sequences. EMBO Journal 6:3787–3794
    [Google Scholar]
  15. Patel D. D., Pickup D. J. 1989; The second largest subunit of the poxvirus RNA polymerase is similar to the corresponding subunits of procaryotic and eucaryotic RNA polymerases. Journal of Virology 63:1076–1086
    [Google Scholar]
  16. Patel D. D., Ray C. A., Drucker R. P., Pickup D. J. 1988; A poxvirus-derived vector that directs high levels of expression of cloned genes in mammalian cells. Proceedings of the National Academy of Sciences, U.S.A 85:9431–9435
    [Google Scholar]
  17. Robinson A. J., Balassu T. C. 1981; Contagious pustular dermatitis (orf). Veterinary Bulletin 51:771–779
    [Google Scholar]
  18. Robinson A. J., Barns G., Fraser K., Carpenter E., Mercer A. A. 1987; Conservation and variation of orf virus genome. Virology 157:13–23
    [Google Scholar]
  19. Rodriguez J. F., Esteban M. 1987; Mapping and nucleotide sequence of the vaccinia virus gene that encodes a 14-kilodaIton fusion protein. Journal of Virology 61:3550–3554
    [Google Scholar]
  20. Rodriguez J. F., Smith G. L. 1990; IPTG-dependent vaccinia virus: identification of a virus protein enabling virion envelopment by Golgi membrane and egress. Nucleic Acids Research 18:5347–5351
    [Google Scholar]
  21. Rodriguez J. F., Janeczko R., Esteban M. 1985; Isolation and characterization of neutralising monoclonal antibodies to vaccinia virus. Journal of Virology 56:482–488
    [Google Scholar]
  22. Rodriguez J. F., Paez E., Esteban M. 1987; A 14, 000-M, envelope protein of vaccinia virus is involved in cell fusion and forms covalently linked trimers. Journal of Virology 61:395–404
    [Google Scholar]
  23. Rohrmann G., Yuen L., Moss B. 1986; Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence. Cell 46:1029–1035
    [Google Scholar]
  24. Rosel J. L., Earl P. L., Weir J. P., Moss B. 1986; Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the Hin dIII H genome fragment. Journal of Virology 60:436–449
    [Google Scholar]
  25. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. 1985; Enzymatic amplification of (β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
    [Google Scholar]
  26. Schmitt J. F. C., Stunnenberg H. G. 1988; Sequence and transcriptional analysis of the vaccinia virus Hin dIII I fragment. Journal of Virology 62:1889–1897
    [Google Scholar]
  27. Yuen L., Moss B. 1987; Oligonucleotide sequence signaling transcriptional termination of vaccinia virus early genes. Proceedings of the National Academy of Sciences, U.S.A. 84:6417–6421
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-5-1177
Loading
/content/journal/jgv/10.1099/0022-1317-72-5-1177
Loading

Data & Media loading...

Most cited Most Cited RSS feed