Cellular and humoral immune responses to poliovirus in mice: a role for helper T cells in heterotypic immunity to poliovirus Free

Abstract

Immunization of BALB/c mice with a single dose of the Sabin type 1, type 2 or type 3 poliovirus vaccine strains stimulated cross-reactive T helper cell responses detected by both proliferation and interleukin (IL)-2/IL-4 production. Although the polyclonal T cell responses were cross-reactive, the results also suggest that a proportion of the T cells were directed against serotype-specific determinants. In contrast, neutralizing antibodies, assayed in the serum from the same animals, were predominantly serotype-specific and only reached significant titres after secondary immunization. A comparison of the immunogenicity of poliovirus administered subcutaneously in Freund’s complete adjuvant or intraperitoneally as an alum precipitate or without adjuvant, showed that optimum responses were obtained by immunization with virus in the presence of alum. An examination of the effect of heterotypic priming showed that immunization with type 2 virus primed for a secondary antibody response to each of the three serotypes, whereas priming with type 1 or type 3 viruses could only generate a secondary antibody response to the homologous virus or to type 2 virus.

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1991-05-01
2024-03-28
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References

  1. Bottiger M. 1973; Antibody stimulation in individuals without demonstrable poliovirus antibodies following a fifth injection of inactivated poliovirus vaccine. Acta pathologicaet microbiologica Scandinavica B81:795–798
    [Google Scholar]
  2. Bruserud O., Thorsby E. 1985; T lymphocyte responses to coxsackie B4 and mumps virus. I. Influence of HLA-DR restriction elements. Tissue antigens 26:41–50
    [Google Scholar]
  3. Collen T., Pullen L., Doel T. R. 1989; T cell-dependent induction of antibody against foot-and-mouth disease virus in a mouse model. Journal of General Virology 70:395–403
    [Google Scholar]
  4. Filman D. J., Syed R., Chow M., Macadam A. J., Minor P. D., Hogle J. M. 1989; Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8:1567–1579
    [Google Scholar]
  5. Gillis S., Ferm M. M., Ou W., Smith K. A. 1978; T cell growth factor: parameters of production and a quantitative microassay for activity. Journal of Immunology 120:2027–2032
    [Google Scholar]
  6. Glezen W. P., McCollough R. H., Lamb G. A., Chin T. D. Y. 1969; Quantitative relationship of pre-existing homotypic antibodies to excretion of poliovirus types 1, 2 and 3 following the feeding of trivalent attenuated poliovirus vaccine. American Journal of Epidemiology 90:146–156
    [Google Scholar]
  7. Hogle J. M., Chow M., Filman D. J. 1985; The threedimensional structure of poliovirus at 2.9 A resolution. Science 229:1358–1365
    [Google Scholar]
  8. Milich D. R., McLachlan A. 1988; HBc Ag can function both as a T-cell-independent and a T-cell-dependent antigen: HBcAg and HBeAg are cross-reactive at the T cell level. In Viral Hepatitis and Liver Disease pp 645–649 Edited by Zuckerman A. New York: Alan Liss;
    [Google Scholar]
  9. Mills K. H. G. 1989; Recognition of foreign antigen by T cells and their role in immune protection. Current Opinion in Infectious Diseases 2:804–814
    [Google Scholar]
  10. Minor P. D. 1985; Growth, assay and purification of picornaviruses. In Virology - A Practical Approach pp 25–41 Edited by Mahy B. W. J. Oxford: IRL Press;
    [Google Scholar]
  11. Minor P. D. 1990; Antigenic structure of picornaviruses. Current Topics in Microbiology and Immunology 161:121–154
    [Google Scholar]
  12. Page G. S., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
    [Google Scholar]
  13. Paul W. E. 1989; Pleiotrophy and redundancy: T-cell derived lymphokines in the immune response. Cell 57:521–524
    [Google Scholar]
  14. Reinherz E. L., Schlossman S. F. 1981; The characterisation and function of human immunoregulatory T lymphocyte subsets. Immunology Today 2:69–75
    [Google Scholar]
  15. Salk D., Van Wezel A. L., Salk J. 1984; Induction of long-term immunity to paralytic poliomyelitis by use of non-infectious vaccine. Lancet ii:1317–1321
    [Google Scholar]
  16. Scherle P. A., Gerhard W. 1986; Functional analysis of influenza-specific helper T cell clones in vivo. T cells specific for internal viral proteins provide cognate help for B cell responses to haemagglutinin. Journal of Experimental Medicine 164:1114–1128
    [Google Scholar]
  17. Schwartz R. H. 1985; T-lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex. Annual Review of Immunology 3:237–261
    [Google Scholar]
  18. Uhlig J., Wiegers K., Dernick R. 1990; A new antigenic site of poliovirus recognized by an intertypic crossneutralizing monoclonal antibody. Virology 178:606–610
    [Google Scholar]
  19. UytdeHaag F. G. C. M., Loggen H. G., Logtenberg T., Lichtveld R. A., Van Steenis B., Van Asten J. A. A. M., Osterhaus A. D. M. E. 1985; Human peripheral blood lymphocytes from recently vaccinated individuals produce both type-specific and intertypic cross-reacting neutralizing antibody on in vitro stimulation with one type of poliovirus. Journal of Immunology 135:3094–3101
    [Google Scholar]
  20. Wang K., Sun L., Jubelt B., Waltenbaugh C. 1989; Cell-mediated immune responses to poliovirus. I. Conditions for induction, characterization of effector cells, and cross-reactivity between serotypes for delayed hypersensitivity and T cell proliferative responses. Cellular Immunology 119:252–262
    [Google Scholar]
  21. Welsh C. J. R., Blakemore W. F., Tonks P., Borrow P., Nash A. A. 1989; Theiler’s murine encephalomyelitis virus infection in mice: a persistent viral infection of the central nervous system which induces demyelination. In Immune Responses, Virus Infections and Disease pp 125–147 Edited by Dimmock N. J., Minor P. D. Oxford: IRL Press;
    [Google Scholar]
  22. Zinkernagel R. M., Doherty P. C. 1979; MHC-restricted cytotoxic T cells. Advances in Immunology 27:51–177
    [Google Scholar]
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