1887

Abstract

A subgenomic cDNA clone from hepatitis A virus strain HM175, composed of the last eight nucleotides of the 5′ non-translated region and the first 2248 nucleotides of the coding sequence (P1 region), was inserted into a vector under the control of the T7 promoter. Restriction enzyme digestion at sites within the structural region and subsequent transcription yielded RNA products which were translated efficiently in rabbit reticulocyte lysates to produce proteins of the predicted sizes. The translation products were specifically precipitated with antipeptide antisera; these reactions were not affected by denaturation of the antigens by boiling in 1% SDS. The translated proteins were also precipitated by antivirion antisera, but recognition was totally abolished following denaturation. Thus antivirion antisera recognized conformation-dependent epitopes expressed on the translated products exclusively.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-5-1081
1991-05-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/5/JV0720051081.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-5-1081&mimeType=html&fmt=ahah

References

  1. Clarke B. E., Sangar D. V. 1988; Processing and assembly of foot-and-mouth disease virus proteins using subgenomic RNA. Journal of General Virology 69:2313–2325
    [Google Scholar]
  2. Cohen J. I., Ticehurst J. R., Purcell R. H., Buckler-White A., Baroudy B. M. 1987a; Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picomaviruses. Journal of Virology 61:50–59
    [Google Scholar]
  3. Cohen J. I., Rosenblum B., Ticehurst J. R., Daemer R. J., Feinstone S. M., Purcell R. H. 1987b; Complete nucleotide sequence of an attenuated hepatitis A virus: comparison with wild- type virus. Proceedings of the National Academy of Sciences, U.S.A 84:2497–2501
    [Google Scholar]
  4. Coulepis A. G., Locarnini S. A., Westaway E. G., Tannock G. A., Gust I. D. 1982; Biophysical and biochemical characterization of hepatitis A virus. Intervirology 18:107–127
    [Google Scholar]
  5. Dorner A. J., Semler B. L., Jackson R. J., Hanecak R., Duprey E., Wimmer E. 1984; In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate. Journal of Virology 50:507–514
    [Google Scholar]
  6. Emini E. A., Hughes J. V., Perlow D. S., Boger J. 1985; Induction of hepatitis A virus-neutralizing antibody by a virus- specific synthetic peptide. Journal of Virology 55:836–839
    [Google Scholar]
  7. Gauss-Muller V., Mingquan Z., von der Helm K., Deinhardt F. 1990; Recombinant proteins VP1 and VP3 of hepatitis A virus prime for neutralizing response. Journal of Medical Virology 31:277–283
    [Google Scholar]
  8. Hogle J. M., Chow M., Filman D. J. 1985; Three-dimensional structure of poliovirus at 2.9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  9. Houghten R. A. 1985; General method for the rapid solid phase synthesis of large numbers of peptides. Specificity of antigen–antibody interactions at the level of individual amino acids. Proceedings of the National Academy of Sciences, U.S.A 82:5131–5135
    [Google Scholar]
  10. Hughes J. V., Stanton L. W., Tomassini J. E., Long W. J., Scolnick E. M. 1984; Neutralizing monoclonal antibodies to hepatitis A virus: partial localization of a neutralizing antigenic site. Journal of Virology 52:465–473
    [Google Scholar]
  11. Hughes J. V., Bennett C., Stanton L., Linemeyer D. L., Mitra S. W. 1985; Hepatitis A virus structural proteins: sequencing and ability to induce virus-neutralizing antibody responses. In Vaccines 85: Molecular and Chemical Basis of Resistance to Parasitic, Bacterial and Viral Diseases pp 255–259 Edited by Lemer R. A., Chanock R. M., Brown F. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Jansen R. W., Newbold J. E., Lemon S. M. 1988; Complete nucleotide sequence of a cell culture-adapted variant of hepatitis A virus: comparison with wild-type virus with restricted capacity for in vitro replication. Virology 163:299–307
    [Google Scholar]
  13. Johnston J., Harmon S. A., Binn L. N., Richards O. C., Ehrenfeld E., Summers D. F. 1988; Antigenic and immunogenic properties of a hepatitis A virus capsid protein expressed in Escherichia coli . Journal of Infectious Diseases 157:1203–1211
    [Google Scholar]
  14. Karayiannis P., O’Rourke S., McGarvey M. J., Luther S., Waters J., Goldin R., Thomas H. C. 1991; Vaccination of tamarins with a recombinant vaccinia–hepatitis A virus protects against HAV infection. In The 1990 International Symposium on Viral Hepatitis and Liver Disease (in press)
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. Lemon S. M., Binn L. N. 1983; Antigenic relatedness of two strains of hepatitis A virus determined by cross-neutralization. Infection and Immunity 42:418–420
    [Google Scholar]
  17. Lemon S. M., Binn L. N., Marchwicki R., Murphy P. C., Ping L.-H., Jansen R. W., Asher L. V. S., Stapleton J. T., Taylor D. G., LeDuc J. W. 1990; In vivo replication and reversion to wild-type of a neutralization- resistant variant of hepatitis A virus. Journal of Infectious Diseases 161:7–13
    [Google Scholar]
  18. Liu F., Zinnecker M., Hamaoka T., Katz D. H. 1979; New procedure for preparation and isolation of conjugates of protein and a synthetic co-polymer of d amino acids and immunochemical characterization of such conjugates. Biochemistry 18:690–697
    [Google Scholar]
  19. MacGregor A., Kornitschuk M., Hurrell J. G. R., Lehmann N. I., Coulepis A. G., Locarnini S. A., Gust I. D. 1983; Monoclonal antibodies against hepatitis A virus. Journal of Clinical Microbiology 18:1237–1243
    [Google Scholar]
  20. Melnick J. L. 1990; New picomavirus vaccines for hepatitis A and lessons from the control of poliomyelitis by the prototype picomavirus vaccines. Progress in Medical Virology 37:47–55
    [Google Scholar]
  21. Nicklin M. J. H., Krausslich H. G., Toyoda H., Dunn J. J., Wimmer E. 1987; Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinases. Proceedings of the National Academy of Sciences, U.S.A 84:4002–4006
    [Google Scholar]
  22. Nielsen D. A., Shapiro D. J. 1986; Preparation of capped RNA transcripts using T7 RNA polymerase. Nucleic Acids Research 14:5936
    [Google Scholar]
  23. Ostermayr R., von der Helm K., Gauss-Muller V., Winnacker D. L., Deinhardt F. 1987; Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein. Journal of Virology 61:3645–3647
    [Google Scholar]
  24. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  25. Ping L.-H., Jansen R. W., Stapleton J. T., Cohen J. I., Lemon S. M. 1988; Identification of an immunodominant antigenic site involving the capsid protein VP3 of hepatitis A virus. Proceedings of the National Academy of Sciences, U.S.A 85:8281–8285
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd. edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  28. Stapleton J. T., Lemon S. M. 1987; Neutralization escape mutants define a dominant immunogenic neutralization site on hepatitis A virus. Journal of Virology 61:491–498
    [Google Scholar]
  29. Stapleton J. T., Jansen R. W., Lemon S. M. 1985; Neutralizing antibody to hepatitis A virus in immune serum globulin and in the sera of human recipients of immune serum globulin. Gastroenterology 89:637–642
    [Google Scholar]
  30. Ticehurst J. R., Racaniello V. R., Baroudy B. M., Baltimore D., Purcell R. H., Feinstone S. M. 1983; Molecular cloning and characterization of hepatitis A virus cDNA. Proceedings of the National Academy of Sciences, U.S.A 80:5885–5889
    [Google Scholar]
  31. Ticehurst J., Cohen J. I., Feinstone S. M., Purcell R. H., Jansen R. W., Lemon S. M. 1989; Replication of hepatitis A virus: new ideas from studies with cloned cDNA. In Molecular Aspects of Picomavirus Infection and Detection pp 27–49 Edited by Semler B. L., Ehrenfeld E. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-5-1081
Loading
/content/journal/jgv/10.1099/0022-1317-72-5-1081
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error