@article{mbs:/content/journal/jgv/10.1099/0022-1317-72-4-995, author = "Kashiwazaki, Satoshi and Minobe, Yuzo and Hibino, Hiroyuki", title = "Nucleotide sequence of barley yellow mosaic virus RNA 2", journal= "Journal of General Virology", year = "1991", volume = "72", number = "4", pages = "995-999", doi = "https://doi.org/10.1099/0022-1317-72-4-995", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-4-995", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The sequence of the 3585 nucleotides [excluding the 3′ poly(A) tail] of barley yellow mosaic virus (BaYMV) RNA 2 was obtained by analysis of cDNA clones and by direct RNA sequencing. The first initiation codon at nucleotide 155 was followed by a single long open reading frame encoding a protein of 890 amino acids with an M r of 98458. Amino acid sequence comparisons indicated that the BaYMV 98K protein contains a region similar to the C-terminal proteinase domain of the potyvirus helper component (HC) protein towards its N terminus, but that it has no sequences that resemble the N-terminal part of the HC protein or other proteins of potyviruses. The data reveal striking differences in genetic organization between BaYMV RNA 2 and the 5′-terminal region of the potyvirus genome, despite a close genetic relationship between BaYMV RNA 1 and the rest of the potyvirus genome.", }