1887

Abstract

Proteolytic activation of Sendai virus in the lungs of mice is necessary to cause pneumopathogenicity. Using Sendai virus-infected lung block cultures, protease inhibitors were tested for their antiviral effect by examining inhibition of proteolytic activation. Among the inhibitors tested, a serine protease, aprotinin, was shown to be most effective. protection experiments demonstrated that aprotinin, when administered intranasally, could confer protection on mice against lethal Sendai virus pneumonia through the same mechanism as observed in the system. The present study provides an experimental basis for the use of protease inhibitors as antiviral drugs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-4-979
1991-04-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/4/JV0720040979.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-4-979&mimeType=html&fmt=ahah

References

  1. Homma M. 1971; Trypsin action on the growth of Sendai virus in tissue culture cells. I. Restoration of the infectivity for L cells by direct action of trypsin on L cell-borne Sendai virus. Journal of Virology 8:619–629
    [Google Scholar]
  2. Homma M. 1975; Host-induced modification of Sendai virus. In Negative-Strand Viruses pp 685–697 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  3. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural difference of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
    [Google Scholar]
  4. Homma M., Tamagawa S. 1973; Restoration of the fusion activity of L cell-borne Sendai virus by trypsin. Journal of General Virology 19:423–426
    [Google Scholar]
  5. Homma M., Tozawa H., Shimizu K., Ishida N. 1975; A proposal for designation of Sendai virus proteins. Japanese Journal of Microbiology 19:467–470
    [Google Scholar]
  6. Ishida N., Homma M. 1978; Sendai virus. Advances in Virus Research 23:349–383
    [Google Scholar]
  7. Itoh M., De Ming T., Hayashi T., Mochizuki Y., Homma M. 1990; Pneumopathogenicity of a Sendai virus protease-activation mutant, TCs, which is sensitive to trypsin and chymotrypsin. Journal of Virology 64:5660–5664
    [Google Scholar]
  8. Katoh I., Yasunaga T., Ikawa Y., Yoshinaka Y. 1987; Inhibition of retroviral protease activity by an aspartyl proteinase inhibitor. Nature, London 329:654–656
    [Google Scholar]
  9. Korant B. D., Brzin J., Turk U. 1985; Cystatin, a protein inhibitor of cysteine protease alters viral protein cleavages in infected human cells. Biochemical and Biophysical Research Communications 127:1072–1076
    [Google Scholar]
  10. Mountcastle W. E., Compans P. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  11. Ohuchi M., Homma M. 1976; Trypsin action on the growth of Sendai virus in tissue culture cells. IV. Evidence for activation of Sendai virus by cleavage of a glycoprotein. Journal of Virology 18:1147–1150
    [Google Scholar]
  12. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  13. Tashiro M., Homma M. 1983a; Pneumotropism of Sendai virus in relation to protease-mediated activation in mouse lungs. Infection and Immunity 39:879–888
    [Google Scholar]
  14. Tashiro M., Homma M. 1983b; Evidence of proteolytic activation of Sendai virus in mouse lung. Archives of Virology 77:127–137
    [Google Scholar]
  15. Tashiro M., Homma M. 1985; Protection of mice from wild-type Sendai virus infection by a trypsin-resistant mutant, TR-2. Journal of Virology 53:228–234
    [Google Scholar]
  16. Tashiro M., Klenk H.-D., Rott R. 1987; Inhibitory effect of a protease inhibitor, leupeptin, on the development of influenza pneumonia, mediated by concomitant bacteria. Journal of General Virology 68:2039–2041
    [Google Scholar]
  17. Yuasa Y., Shimojo H., Aoyagi T., Umezawa H. 1975; Effect of protease inhibitors on focus formation by murine sarcoma virus. Journal of the National Cancer Institute 54:1255–1256
    [Google Scholar]
  18. Zhirnov O. P., Ovcharenko A. V., Bukrinskaya A. G. 1984; Suppression of influenza virus replication in infected mice by protease inhibitors. Journal of General Virology 65:191–196
    [Google Scholar]
  19. Zhirnov O. P., Ovcharenko A. V., Bukrinskaya A. G. 1985; Myxovirus replication in chicken embryos can be suppressed by aprotinin due to the blockage of viral glycoprotein cleavage. Journal of General Virology 66:1633–1638
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-4-979
Loading
/content/journal/jgv/10.1099/0022-1317-72-4-979
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error