@article{mbs:/content/journal/jgv/10.1099/0022-1317-72-4-955, author = "Merrick, Ruth M. and Grand, Roger J. A. and Brown, Judith C. and Gallimore, Phillip H.", title = "The use of β-galactosidase fusion proteins encoding the early region 1 transforming proteins of adenovirus type 12 to examine the humoral response in tumour-bearing animals", journal= "Journal of General Virology", year = "1991", volume = "72", number = "4", pages = "955-960", doi = "https://doi.org/10.1099/0022-1317-72-4-955", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-4-955", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Sera from 26 rats bearing tumours induced by wild-type (wt) and mutant human adenovirus type 12 (Ad12), or by cells transformed with these viruses, were analysed for antibodies against the early region 1 (E1) transforming proteins. Six Ad12-β-galactosidase fusion proteins encoding different regions of the Ad12 E1 proteins were constructed. The sera from the tumour-bearing animals reacted most strongly with the fusion protein encoding the N terminus of the E1A protein. Tumour-bearing rats exposed to the E1B 54K and 19K proteins showed strong reactions with the N terminus of the 54K protein and the C terminus of the 19K protein. Monospecific polyclonal antisera were raised against five of the fusion proteins by immunization of rats and rabbits; these sera cross-reacted with the purified native protein. No antibodies could be obtained which recognized a fusion protein containing amino acids 136 to 268 of the 54K protein. The fusion proteins were also used to purify monospecific antisera from tumour-bearer sera using affinity chromatography.", }