1887

Abstract

Sera from 26 rats bearing tumours induced by wild-type (wt) and mutant human adenovirus type 12 (Ad12), or by cells transformed with these viruses, were analysed for antibodies against the early region 1 (E1) transforming proteins. Six Ad12--galactosidase fusion proteins encoding different regions of the Ad12 E1 proteins were constructed. The sera from the tumour-bearing animals reacted most strongly with the fusion protein encoding the N terminus of the E1A protein. Tumour-bearing rats exposed to the E1B 54K and 19K proteins showed strong reactions with the N terminus of the 54K protein and the C terminus of the 19K protein. Monospecific polyclonal antisera were raised against five of the fusion proteins by immunization of rats and rabbits; these sera cross-reacted with the purified native protein. No antibodies could be obtained which recognized a fusion protein containing amino acids 136 to 268 of the 54K protein. The fusion proteins were also used to purify monospecific antisera from tumour-bearer sera using affinity chromatography.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-4-955
1991-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/4/JV0720040955.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-4-955&mimeType=html&fmt=ahah

References

  1. Berk A. J., Sharp P. A. 1978; Structure of the adenovirus 2 early mRNAs. Cell 14:695–711
    [Google Scholar]
  2. Bos J. L., Polder L. J., Bernards R., Schrier P., van den Elsen P. J., van der Eb A. J., van Ormondt H. 1981; The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumour antigens starting at different AUG triplets. Cell 27:121–131
    [Google Scholar]
  3. Byrd P. J., Brown K. W., Gallimore P. H. 1982; Malignant transformation of human embryo retinoblasts by cloned adenovirus 12 DNA. Nature, London 298:69–71
    [Google Scholar]
  4. Byrd P. J., Grand R. J. A., Breiding D., Williams J. F., Gallimore P. H. 1988; Host range mutants of adenovirus type 12 E1 defective for lytic infection, transformation and oncogenicity. Virology 163:155–165
    [Google Scholar]
  5. Doorbar J., Campbell D., Grand R. J. A., Gallimore P. H. 1986; Identification of the human papilloma virus-la E4 gene products. EMBO Journal 5:355–362
    [Google Scholar]
  6. Doorbar J., Evans H. S., Coneron I., Crawford L. V., Gallimore P. H. 1988; Analysis of HPV-1 E4 gene expression using epitope-defined antibodies. EMBO Journal 7:355–362
    [Google Scholar]
  7. Gallimore P. H., Williams J. 1982; An examination of adenovirus type 5 mutants for their ability to induce group C adenovirus tumour-specific transplantation antigenicity in rats. Virology 120:146–156
    [Google Scholar]
  8. Gallimore P. H., Sharp P. A., Sambrook J. 1974; Viral DNA in transformed cells: a study of the sequence of adenovirus 2 DNA in nine lines of transformed rat cells using specific fragments of the viral genome. Journal of Molecular Biology 89:49–72
    [Google Scholar]
  9. Gallimore P. H., Byrd P. J., Whittaker J. L., Grand R. J. A. 1985; Properties of rat cell transformed by DNA plasmids containing adenovirus type 12 E1 DNA or specific fragments of the E1 region: comparison of transforming frequencies. Cancer Research 45:2670–2680
    [Google Scholar]
  10. Grand R. J. A., Gallimore P. H. 1984; Adenovirus type 12 early region 1 proteins: a study of their subcellular localization and protein–protein interactions. Journal of General Virology 65:2149–2166
    [Google Scholar]
  11. Jochemsen H., Daniels G. S. G., Lupker J. H., van der Eb A. J. 1980; Identification and mapping of the early gene products of adenovirus type 12. Virology 105:551–563
    [Google Scholar]
  12. Kast W. M., Offringa R., Peters P. J., Voordouw A. C., Meloen R. H., van der Eb A. J., Melief C. J. M. 1989; Eradication of adenovirus E1-induced tumours by E1A-specific cytotoxic T lymphocytes. Cell 59:603–614
    [Google Scholar]
  13. Lucher L. A., Brackmann K. H., Symington J. S., Green M. 1984; Antibody directed to a synthetic peptide encoding the NH2-terminal 16 amino acids of the adenovirus type 2 E1B-53K tumor antigen recognizes the E1B-20K tumor antigen. Virology 132:217–221
    [Google Scholar]
  14. Moran E., Mathews M. B. 1987; Minireview: multiple functional domains in the adenovirus Ela gene. Cell 48:177–178
    [Google Scholar]
  15. Padlan E. A., Silverton E. W., Sheriff S., Cohen G. H., Smith-Gill S. J., Davies D. R. 1989; Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex. Proceedings of the National Academy of Sciences, U.S.A. 86:5938–5942
    [Google Scholar]
  16. Paraskeva C., Brown K. W., Gallimore P. H. 1982; Adenovirus-cell interactions early after infection: in vitro characteristics and tumourigenicity of adenovirus type 2-transformed rat liver epithelial cells. Journal of General Virology 58:73–81
    [Google Scholar]
  17. Raska K. Jr, Gallimore P. H. 1982; An inverse relation of the oncogenic potential of adenovirus-transformed cells and their sensitivity to killing by syngeneic natural killer cells. Virology 123:8–18
    [Google Scholar]
  18. Raska K. Jr, Morrongiello M. P., Fohring B. 1980; Adenovirus type-12 tumour antigen. III. Tumorigenicity and immune response to syngeneic rat cells transformed with virions and isolated transforming fragment of adenovirus 12 DNA. International Journal of Cancer 26:79–86
    [Google Scholar]
  19. Rowe D. T., Yee S., Otis J., Graham F. L., Branton P. E. 1983; Characterization of human adenovirus type 5 early region 1A polypeptides using antitumor sera and an antiserum specific for the carboxy terminus. Virology 127:253–271
    [Google Scholar]
  20. Scott M. O., Kimelman D., Norris D., Ricciardi R. P. 1984; Production of a monospecific antiserum against the early region 1A proteins of adenovirus 12 and adenovirus 5 by an adenovirus 12 early region 1A-β-galactosidase fusion protein antigen expressed in bacteria. Journal of Virology 50:895–903
    [Google Scholar]
  21. Schugart K., Bause E., Esche H. 1985; Structure and expression of adenovirus type 12 E1B 58K protein in infected and transformed cells: studies using antibodies directed against a synthetic peptide. Virus Research 3:41–56
    [Google Scholar]
  22. Schugart K., von Wilcken-Bergmann B., Esche H. 1987; Expression of adenovirus type 12 E1b 58-kDa protein in Escherichia coli and production of antibodies raised against a 58 kDa: β-galactosidase fusion protein. Gene 53:173–180
    [Google Scholar]
  23. Smith K. J., Gallimore P. H., Grand R. J. A. 1989; The expression of Ad12 E1B 18K protein on the surface of adenovirus transformed and infected human cells. Oncogene 4:489–497
    [Google Scholar]
  24. Tsukamoto A. S., Ferguson B., Rosenberg M., Weissman I. L., Berk A. J. 1986; An immunodominant domain in adenovirus type 2 early region 1A proteins. Journal of Virology 60:312–316
    [Google Scholar]
  25. van der Eb A. J., Mulder C., Graham F. L., Houweling A. 1977; Transformation with specific fragments of adenovirus DNA’s: isolation of specific fragments with transforming ability of adenovirus 2 and 5 DNA. Gene 2:115–132
    [Google Scholar]
  26. van der Eb A. J., van Ormondt H., Schrier P. I., Lupker J. H., Jochemsen H., van den Elsen P. J., Deleys J., Maat J., van Beveren C. P., Dijkema R., de Waard A. 1979 Cold Spring Harbor Symposia on Quantitative Biology 44:383–398
    [Google Scholar]
  27. Yano S., Ojima S., Fujinaga K., Shiroki K., Shimojo H. 1977; Transformation of a rat cell line by an adenovirus type 12 DNA fragment. Virology 82:214–220
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-4-955
Loading
/content/journal/jgv/10.1099/0022-1317-72-4-955
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error