The DNA sequence of a 5.5 kbp EcoRI fragment located in the short unique region (US) of the ‘highly oncogenic’ strain RB1B of Marek’s disease virus (MDV) was determined. The sequence contained six open reading frames (ORFs), four of which were homologous to proteins mapping in the US region of herpes simplex virus type 1 (HSV-1). These include the homologues of HSV-1 protein kinase, glycoprotein D (gD), glycoprotein I (gI) and US2 which is of unknown function. The MDV ORFs had a marked bias for A or T in the third codon position and analysis of the dinucleotide frequencies showed a marginal deficit in ApG/CpT but no overall deviation of CpG from random expectations. Comparison of genes in the US region of MDV to herpesvirus proteins confirmed and extended our previous observation that MDV is more closely related to alphaherpesviruses than to gamma-herpesviruses. We also showed that MDV possessed a homologue of HSV-1 gD which is lacking in varicellazoster virus (VZV) but that MDV probably lacked homologues of US4 and US5 of HSV-1. These results show that in contrast to the genes in the long unique region which were grossly collinear in HSV, VZV and MDV, those mapping in US show greater diversity.
BinnsM. M.,
RossL. J. N.1989; Nucleotide sequence of the Marek’s disease virus (MDV) RB-1B A antigen gene and the identification of the MDV A antigen as the herpes simplex virus-1 glycoprotein C homologue. Virus Research 12:371–382
BinnsM. M.,
StenzlerL.,
TomleyF. M.,
CampbellJ.,
BoursnellM. E. G.1987; Identification by a random sequencing strategy of the fowlpox virus DNA polymerase gene, its nucleotide sequence and comparison with viral polymerases. Nucleic Acids Research 15:6563–6573
BuckmasterA. E.,
ScottS. D.,
SandersonM. J.,
BoursnellM. E. G.,
RossL. J. N.,
BinnsM. M.1988; Gene sequence and mapping data from Marek’s disease virus and herpesvirus of turkeys: implications for herpesvirus classification. Journal of General Virology 69:2033–2042
CebrianJ.,
Kascha-DierichC.,
BerthelotN.,
SheldrickP.1982; Inverted repeat nucleotide sequences in the genomes of Marek’s disease virus and the herpesvirus of the turkey. Proceedings of the National Academy of Sciences, U.S.A 79:555–558
CoussensP. M.,
VelicerL. F.1988; Structure and complete nucleotide sequence of Marek’s disease herpesvirus gp57-65 gene. Journal of Virology 62:2372–2379
DavisonA. J.,
McGeochD. J.1986; Evolutionary comparisons of the S segments in the genomes of herpes simplex virus type 1 and varicella-zoster virus. Journal of General Virology 67:597–611
GranthamR.,
GautierC.,
GouyM.,
JacobzoneM.,
MercierR.1981; Codon catalog usage is a genome strategy modulated for gene expression. Nucleic Acids Research 9:r43–r47
HonessR. W.,
GompelsU. A.,
BarrellB. G.,
CraxtonM.,
CameronK. R.,
StadenR.,
ChangY.-N.,
HaywardG. S.1989; Deviations from expected frequencies of CpG dinucleotides in herpesvirus DNAs may be diagnostic of differences in the states of their latent genomes. Journal of General Virology 70:837–855
McGeochD. J.1990; Evolutionary relationships of virion glycoprotein genes in the S regions of alphaherpesvirus genomes. Journal of General Virology 71:2361–2367
McGeochD. J.,
DolanA.,
DonaldS.,
RixonF.1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–14
McGeochD. J.,
MossH. W. M.,
McNabD.,
FrameM. C.1987; DNA sequence and genetic content of the Hin dIII l region in the short unique component of the herpes simplex virus type 2 genome: identification of the gene encoding glycoprotein G, and evolutionary comparisons. Journal of General Virology 68:19–38
McGeochD. J.,
DalrympleM. A.,
DavisonA. J.,
DolanA.,
FrameM. C.,
McNabD.,
PerryL. J.,
ScottJ. E.,
TaylorP.1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
RossL. J. N.,
BinnsM. M.1991; Properties and evolutionary relationships of the Marek’s disease virus homologues of protein kinase, glycoprotein D and glycoprotein I of herpes simplex virus. Journal of General Virology 72:939–947
RossL. J. N.,
SandersonM.,
ScottS. D.,
BinnsM. M.,
DoelT.,
MilneB.1989; Nucleotide sequence and characterization of the Marek’s disease virus homologue of glycoprotein B of herpes simplex virus. Journal of General Virology 70:1789–1804
ScottS. D.,
RossL. J. N.,
BinnsM. M.1989; Nucleotide and predicted amino acid sequences of the Marek’s disease virus and turkey herpesvirus thymidine kinase genes; comparison with thymidine kinase genes of other herpesviruses. Journal of General Virology 70:3055–3065
StadenR.1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
StadenR.,
McLachlanA. D.1982; Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Research 10:141–156
TedderD. G.,
EverettR. D.,
WilcoxK. W.,
BeardP.,
PizerL. I.1989; ICP4-binding sites in the promoter and coding regions of the herpes simplex virus gD gene contribute to activation of in vitro transcription by ICP4. Journal of Virology 63:2510–2520
Van ZiilM.,
van der GuldenH.,
de WindN.,
GielkensA.,
BernsA.1990; Identification of two genes in the unique short region of pseudorabies virus; comparison with herpes simplex virus and varicella-zoster virus. Journal of General Virology 71:1747–1755
WhittonJ. L.,
ClementsJ. B.1984; The junctions between the repetitive and the short unique sequences of the herpes simplex virus genome are determined by the polypeptide-coding regions of two spliced immediate-early mRNAs. Journal of General Virology65451–466