1887

Abstract

RNA transcription in eight human papillomavirus (HPV) type 16-positive genital carcinomas and in two cervical squamous cell carcinoma (SCC)-derived cell lines was analysed by hybridization using I-labelled subgenomic riboprobes. Transcripts corresponding to the E6 and E7 open reading frames were always present, except within the keratinizing layers of differentiated SCCs. Intranuclear E1 gene transcripts were detectable in both cell lines and some tumours whereas E2/E4 transcripts were absent from five of seven assessable tumours, suggesting transcription from integrated viral DNA. When mRNA sense riboprobes were used as controls, no signal was seen in the two cell lines; however, three tumours contained a focal, intense nuclear RNase-sensitive signal using mRNA sense riboprobes. This antisense RNA signal mapped across the whole genome including the non-coding region. In one tumour, the presence of antisense RNA was independently confirmed by using E7 and E2/E4 RNA probes of both orientations in RNase protection assays. Transcription of antisense RNA may be a natural feature of some HPV-positive genital tumours and its possible role in modulating cell behaviour requires further investigation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-4-885
1991-04-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/4/JV0720040885.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-4-885&mimeType=html&fmt=ahah

References

  1. Baker C. C., Phelps W. C., Lindgren V., Braun M. J., Gonda M. A., Howley P. M. 1987; Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. Journal of Virology 61:962–971
    [Google Scholar]
  2. Banks L., Matlashewski G., Pim D., Churcher M., Roberts C., Crawford L. 1987; Expression of human papillomavirus type 6 and type 16 capsid proteins in bacteria and their antigenic characterization. Journal of General Virology 68:3081–3089
    [Google Scholar]
  3. Boshart M., Gissmann L., Ikenberg H., Kleinheinz A., Scheurlen W., zur Hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:1151–1157
    [Google Scholar]
  4. Chomczynski P., Sacchi N. 1987; Single step method of RNA isolation by acid guanidinium thiocyanate-phenol–chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  5. Choo K.-B., Pan C.-C., Han S.-H. 1987; Integration of human papillomavirus type 16 into cellular DNA of cervical carcinoma; preferential deletion of the E2 gene and invariable retention of the long control region and the E6/E7 open reading frame. Virology 161:259–261
    [Google Scholar]
  6. Chow L. T., Hirochika H., Nasseri M., Stoler M. H., Wolinsky S. M., Chin M. T., Hirochika R., Arvan D. S., Broker T. R. 1987; Human papillomavirus gene expression. In Cancer Cells vol 5 Papillomaviruses, pp 55–72 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Crum C. P., Nuovo G., Friedman D., Silverstein S. J. 1988; Accumulation of RNA homologous to human papilloma virus type 16 open reading frames in genital precancers. Journal of Virology 62:84–90
    [Google Scholar]
  8. De Villiers E. M. 1989; Heterogeneity of the human papillomavirus group. Journal of Virology 63:4898–4903
    [Google Scholar]
  9. Dürst M., Gissmann L., Ikenberg H., zur Hausen H. 1983; A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proceedings of the National Academy of Sciences, U,. S,. A 80:3812–3815
    [Google Scholar]
  10. Gissmann L., Wolnik L., Ikenberg H., Koldovsky U., Schnurch H. G., zur Hausen H. 1983; Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proceedings of the National Academy of Sciences, U,. S,. A 80:560–563
    [Google Scholar]
  11. Hawley-Nelson P., Vousden K. H., Hubbert N. L., Lowy D. R., Schiller J. T. 1989; HPV 16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. EMBO Journal 8:3905–3910
    [Google Scholar]
  12. Hitt M. M., Allday M. J., Hara T., Karran L., Jones M. D., Busson P., Tursz T., Ernberg I., Griffin B. E. 1989; EBV expression in an NPC–related tumour. EMBO Journal 8:2639–2651
    [Google Scholar]
  13. Ho D. Y., Mocarski E. S. 1989; Herpes simplex virus latent RNA (LAT) is not required for latent infection in the mouse. Proceedings of the National Academy of Sciences, U,. S,. A 86:7596–7600
    [Google Scholar]
  14. Inouye M. 1988; Antisense RNA: its functions and applications in gene regulation – a review. Gene 72:25–34
    [Google Scholar]
  15. Khochobin S., Lawrence J.-J. 1989; An antisense RNA involved in p53 mRNA maturation in murine erythroleukemia cells induced to differentiate. EMBO Journal 8:4107–4114
    [Google Scholar]
  16. Kimelman D., Kirschner M. W. 1989; An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in xenopus oocytes. Cell 59:687–696
    [Google Scholar]
  17. Kreig P. A., Melton D. A. 1987; In vitro RNA synthesis with SP6 RNA polymerase. Methods in Enzymology 155:397–415
    [Google Scholar]
  18. Lorincz A. T., Temple G. F., Kurman R. J., Jenson A. B., Lancaster W. D. 1987; Oncogenic association of specific human papillomavirus types with cervical neoplasia. Journal of the National Cancer Institute 79:671–677
    [Google Scholar]
  19. McCance D. J., Walker P. G., Dyson J. L., Coleman D. U., Singer A. 1983; Presence of human papillomavirus DNA in cervical intraepithelial neoplasia. British Journal of Medicine 287:784–788
    [Google Scholar]
  20. McCance D. J., Campion M. J., Clarkson P. K., Chesters P. M., Jenkins D., Singer A. 1985; Prevalence of human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and invasive carcinoma of the cervix. British Journal of Obstetrics and Gynaecology 92:1101–1105
    [Google Scholar]
  21. Matsukura T., Kanda T., Furuno A., Yoshikawa H., Kawana T., Yoshiike K. 1986; Cloning of monomeric papillomavirus type 16 DNA integrated within cell DNA from a cervical carcinoma. Journal of Virology 58:979–982
    [Google Scholar]
  22. Münger K., Phelps W. C., Bubb V., Howley P. M., Schlegel R. 1989; The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Journal of Virology 63:4417–4421
    [Google Scholar]
  23. Pater M. M., Pater A. 1988; Expression of human papillomavirus types 16 and 18 DNA sequences in cervical carcinoma cell lines. Journal of Medical Virology 26:185–195
    [Google Scholar]
  24. Reid R., Greenberg M., Jenson A. B., Husain M., Willett J., Daoud Y., Temple G., Stanhope C. R., Sherman A. I., Phibbs G. D., Lorincz A. T. 1987; Sexually transmitted papillomavirus infections. I. The anatomic distribution and pathological grade of neoplastic lesions associated with different viral types. American Journal of Obstetrics and Gynecology 156:212–222
    [Google Scholar]
  25. Schneider–Maunoury S., Croissant O., Orth G. 1987; Integration of human papillomavirus type 16 DNA sequences: a possible early event in the progression of genital tumors. Journal of Virology 61:3295–3298
    [Google Scholar]
  26. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature, London 314:111–114
    [Google Scholar]
  27. Seedorf K., Krämmer G., Dürst M., Suhai S., Röwekamp W. G. 1985; Human papillomavirus type 16 DWA sequence. Virology 145:181–185
    [Google Scholar]
  28. Seedorf K., Oltersdorf T., Krämmer G., Röwekamp W. 1987; Identification of early proteins of the human papillomaviruses type 16 (HPV 16) and type 18 (HPV 18) in cervical carcinoma cells. EMBO Journal 6:139–144
    [Google Scholar]
  29. Shirasawa H., Tomita Y., Kubota K., Kasai T., Sekiya S., Takamizawa H., Simizu B. 1988; Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcionomas. Journal of Virology 62:1022–1027
    [Google Scholar]
  30. Smotkin D., Wettstein F. O. 1986; Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein. Proceedings of the National Academy of Sciences, U,. S,. A 83:4680–4684
    [Google Scholar]
  31. Smotkin D., Prokoph H., Wettstein F. O. 1989; Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. Journal of Virology 63:1441–1447
    [Google Scholar]
  32. Spence R. P., Murray A., Banks L., Kelland L. R., Crawford L. 1988; Analysis of human papillomavirus sequences in cell lines recently derived from cervical cancers. Cancer Research 48:324–328
    [Google Scholar]
  33. Stevens J. G., Wagner E. K., Devi-Rao G. B., Cook M. L., Feldman L. T. 1987; RNA complementary to a herpesvirus a gene mRNA is prominent in latently infected neurons. Science 235:1056–1059
    [Google Scholar]
  34. Stoler M. H., Broker T. R. 1986; In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma. Human Pathology 17:1250–1258
    [Google Scholar]
  35. Stoler M. H., Rhodes C. R., Whitbeck A., Chow L. T., Broker T. R. 1990; Gene expression of HPV types 16 and 18 in cervical neoplasia. In Papillomaviruses pp 1–11 Edited by Howley P. M. New York: Wiley–Liss;
    [Google Scholar]
  36. Tsunokawa Y., Takebe N., Nozawa S., Kasamatsu T., Gissmann L., zur Hausen H., Terada M., Sugimura T. 1986; Presence of human papillomavirus type-16 and type-18 DNA sequences and their expression in cervical cancers and cell lines from Japanese patients. International Journal of Cancer 37:499–503
    [Google Scholar]
  37. Tuttleman J. S., Pugh J. C., Summers J. W. 1986; In vitro experimental infection of primary duck hepatocytes cultures with duck hepatitis B virus. Journal of Virology 58:17–25
    [Google Scholar]
  38. von Knebel Doeberitz M., Oltersdorf T., Schwarz E., Gissmann L. 1988; Correlation of modified human papilloma virus early gene expression with altered growth properties in C4–1 cervical carcinoma cells. Cancer Research 48:3780–3786
    [Google Scholar]
  39. Watanabe S., Kanda T., Yoshiike K. 1989; Human papillomavirus type 16 transformation of primary human embryonic fibroblasts requires expression of open reading frames E6 and E7. Journal of Virology 63:965–969
    [Google Scholar]
  40. Yee C., Krishnan–Hewlett I., Baker C. C., Schlegel R., Howley P. M. 1985; Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. American Journal of Pathology 119:361–365
    [Google Scholar]
  41. zur Hausen H. 1989; Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancer. Cancer Research 49:4677–4681
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-4-885
Loading
/content/journal/jgv/10.1099/0022-1317-72-4-885
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error