1887

Abstract

ELISA, cDNA dot blot hybridization and transmission by vector aphids were used to investigate the occurrence and degree of cross-protection produced in oat plants by virus isolates representing five strains or serotypes of barley yellow dwarf virus, namely PAV, MAV, SGV, RPV and RMV. Generally, the degree of cross-protection was positively correlated with the serological relatedness between the isolates. A high degree of cross-protection occurred between NY-MAV and MAV-PS1, two isolates of the MAV serotype; cross-protection was moderate between MAV-PS1 and either P-PAV (a Purdue isolate of the PAV serotype) or NY-SGV; cross-protection between P-PAV and NY-SGV was low. Cross-protection did not occur in other paired inoculations and did not persist in some plants, the challenge virus eventually becoming detectable. The persistence of cross-protection depended on the interval between inoculations with protecting and challenge viruses; longer inoculation intervals enhanced the persistence of cross-protection. Results obtained by ELISA and dot blot hybridization were usually consistent, indicating that cross-protection affected both viral capsid and RNA synthesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-4-791
1991-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/4/JV0720040791.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-4-791&mimeType=html&fmt=ahah

References

  1. Aapola A. I. E., Rochow W. F. 1971; Relationships among three isolates of barley yellow dwarf virus. Virology 46:127–141
    [Google Scholar]
  2. Barbara D. J., Kawata E. E., Ueng P. P., Lister R. M., Larkins B. A. 1987; Production of cDNA clones from the MAV isolate of barley yellow dwarf virus. Journal of General Virology 68:2419–2427
    [Google Scholar]
  3. Beachy R. N., Powell Abel P. P., Nelson R. S., Register J., Tumer N., Fraley R. T. 1987; Genetic engineering of plants for protection against virus diseases. In Plant Resistance to Viruses, Ciba Foundation Symposium vol 133 pp 151–169 Edited by Evered D., Harnett S. New York: John Wiley;
    [Google Scholar]
  4. Clark M. F., Lister R. M., Bar-Joseph M. 1986; ELISA techniques. Methods in Enzymology 118:742–766
    [Google Scholar]
  5. Clement D. L. 1984 Studies on the ecology and epidemiology of barley yellow dwarf virus in Indiana Ph,. D. thesis, Purdue University
    [Google Scholar]
  6. Costa A. S., Muller G. W. 1980; Tristeza control by cross protection: a U.S.-Brazil cooperative success. Plant Disease 64:538–541
    [Google Scholar]
  7. Diaco R., Lister R. M., Hill J. H., Durand D. P. 1986; Demonstration of serological relationships among isolates of barley yellow dwarf virus by using polyclonal and monoclonal antibodies. Journal of General Virology 67:353–362
    [Google Scholar]
  8. Fattouh F. A. 1988; Some properties, relationships, and interactions of barley yellow dwarf viruses. Ph,. D. thesis, Purdue University
    [Google Scholar]
  9. Fulton R. W. 1982; The protective effects of systemic virus infection. In Active Defense Mechanism in Plants pp 231–245 Edited by Wood R. K. S. NATO Advanced Study Institute Series
    [Google Scholar]
  10. Gildow F. E., Ballinger M. E., Rochow W. F. 1983; Identification of double-stranded RNAs associated with barley yellow dwarf virus infection in oats. Phytopathology 73:1570–1572
    [Google Scholar]
  11. Gill C. C., Comeau A. 1977; Synergism in cereals between com leaf aphid-specific and aphid-nonspecific isolates of barley yellow dwarf virus. Phytopathology 67:1388–1392
    [Google Scholar]
  12. Halstead B. E., Gill C. C. 1971; Effect of inoculation of oats with paired combinations of barley yellow dwarf virus isolates. Canadian Journal of Botany 49:577–581
    [Google Scholar]
  13. Hamilton R. I. 1980; Defenses triggered by previous invaders: viruses. In Plant Disease vol 5 pp 279–303 Edited by Horsfall J. G., Cowling E. B. New York: Academic Press;
    [Google Scholar]
  14. Hammond J., Lister R. M., Foster J. E. 1983; Purification, identity and some properties of an isolate of barley yellow dwarf virus from Indiana. Journal of General Virology 64:667–676
    [Google Scholar]
  15. Hsu H. T., Aebig J., Rochow W. F. 1984; Differences among monoclonal antibodies to barley yellow dwarf vims. Phytopathology 74:600–605
    [Google Scholar]
  16. Jedlinski H., Brown C. M. 1965; Cross-protection and mutual exclusion by three strains of barley yellow dwarf vims in A vena sativa L. Virology 26:613–621
    [Google Scholar]
  17. Lei C. H., Lister R. M. 1988; Variants of barley yellow dwarf from a single isolate. Phytopathology 78:1587 (abstract)
    [Google Scholar]
  18. Lister R. M., Sward R. J. 1988; Anomalies in serological and vector relationships of MAV-like isolates of barley yellow dwarf vims from Australia. Phytopathology 78:766–770
    [Google Scholar]
  19. Lister R. M., Hammond J., Clement D. L. 1983; Comparison of intradermal and intramuscular injection for raising plant vims antisera for use in ELISA. Journal of Virological Methods 6:179–182
    [Google Scholar]
  20. Oshima N. 1975; The control of tomato mosaic disease with attenuated vims of tomato strain of TMV. Review of Plant Protection Research 8:126–135
    [Google Scholar]
  21. Rochow W. F. 1970; Barley yellow dwarf vims. CMI/AAB Descriptions of Plant Viruses no 32
    [Google Scholar]
  22. Rochow W. F. 1977; Dependent vims transmission from mixed infections. In Aphids as Virus Vectors pp 253–273 Edited by Harris K. F., Maramorosch K. New York: Academic Press;
    [Google Scholar]
  23. Rochow W. F. 1982; Dependent transmission by aphids of barley yellow dwarf luteovimses from mixed infections. Phytopathology 72:302–305
    [Google Scholar]
  24. Rochow W. F. 1984 In Barley Yellow Dwarf Virus, A Proceedings of the Workshop CIMMYT 1984 pp 204–205 Edited by Burnett P. A. Mexico: CIMMYT;
    [Google Scholar]
  25. Rochow W. F., Carmichael L. K. 1979; Specificity among barley yellow dwarf viruses in enzyme immunosorbent assays. Virology 95:415–420
    [Google Scholar]
  26. Rochow W. F., Duffus J. E. 1981; Luteoviruses and yellow diseases. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 147–170 Edited by Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  27. Sherwood J. L. 1987; Mechanisms of cross-protection between plant virus strains. In Plant Resistance to Viruses, Ciba Foundation Symposium vol 133 pp 136–150 Edited by Evered D., Harnett S. New York: John Wiley;
    [Google Scholar]
  28. Smith H. C. 1963; Interaction between isolates of barley yellow dwarf virus. New Zealand Journal of Agricultural Research 6:343–353
    [Google Scholar]
  29. Waterhouse P. M., Gildow F. E., Johnstone G. R. 1988; Luteovirus group. CMI/AAB Descriptions of Plant Viruses no 339
    [Google Scholar]
  30. Webby C. N., Lister R. M. 1989; Serological relationships between barley yellow dwarf viruses. Phytopathology 79:1214 (abstract)
    [Google Scholar]
  31. Webby G. N., Lister R. M., Gray S. M. 1989; Purification of the RMV and SGV isolates of barley yellow dwarf virus for antiserum production. Phytopathology 79:1174 (abstract)
    [Google Scholar]
  32. Wen F. 1990 Cross protection and heterologous encapsidation among barley yellow dwarf viruses Ph,. D. thesis, Purdue University
    [Google Scholar]
  33. Wen F., Lister R. M. 1988; Cross-protection among barley yellow dwarf viruses. Phytopathology 78:1587 (abstract)
    [Google Scholar]
  34. Wen F., Lister R. M. 1989; Cross protection and relationships among barley yellow dwarf viruses. Phytopathology 79:1174 (abstract)
    [Google Scholar]
  35. Yeh S.-D., Gonsalves D. 1984; Evaluation of induced mutants of papaya ringspot virus for control by cross protection. Phytopathology 74:1086–1091
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-4-791
Loading
/content/journal/jgv/10.1099/0022-1317-72-4-791
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error