1887

Abstract

The RNA 3 of rice stripe virus (RSV) isolate T was cloned and its nucleotide sequence was determined. The complete primary structure was found to consist of 2504 nucleotides. One putative open reading frame (ORF), between nucleotides 2412 and 1444 in the 5′-proximal region of the virus complementary-sense RNA, encoded a 322 amino acid protein with an of 35134 that was identified as the coat protein. The other ORF, between nucleotides 66 and 701 in the 5′-proximal region of the virus-sense RNA, encoded a 211 amino acid protein with an of 23 874. An intergenic non-coding region (742 nucleotides) between the two ORFs contains oligo(A)- and oligo(U)-rich regions that can be arranged into a 126 base pair stem configuration. These results suggest that RSV RNA 3 has an ambisense coding strategy.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-4-763
1991-04-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/4/JV0720040763.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-4-763&mimeType=html&fmt=ahah

References

  1. Auperin D. D., Romanowski V., Galinski M., Bishop D. H. L. 1984; Sequence studies of Pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA. Journal of Virology 52:897–904
    [Google Scholar]
  2. De Haan P., Wagemakers L., Peters D., Goldbach R. 1990; The S RNA segment of tomato spotted wilt virus has an ambisense character. Journal of General Virology 71:1001–1007
    [Google Scholar]
  3. Gingery R. E. 1988; The rice stripe virus. In The Plant Viruses vol 4 The Filamentous Plant Viruses, pp 297–329 Edited by Milne R. G. New York & London: Plenum Press;
    [Google Scholar]
  4. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  5. Hanahan D. 1985; Techniques for transformation of E. coli.. In DNA Cloning: A Practical Approach vol 1 pp 109–135 Edited by Glover D. M. Oxford: IRL Press;
    [Google Scholar]
  6. Hayakawa T., Mizukami M., Nakajima M., Suzuki M. 1989a; Complete nucleotide sequence of RNA 3 from cucumber mosaic virus (CMV) strain O: comparative study of nucleotide sequences and amino acid sequences among CMV strains O, Q, D and Y. Journal of General Virology 70:499–504
    [Google Scholar]
  7. Hayakawa T., Mizukami M., Nakamura I., Suzuki M. 1989b; Cloning and sequencing of RNA-1 cDNA from cucumber mosaic virus strain O. Gene 85:533–540
    [Google Scholar]
  8. Hayano Y., Kakutani T., Hayashi T., Minobe Y. 1990; Coding strategy of rice stripe virus: major nonstructural protein is encoded in viral RNA segment 4 and coat protein in RNA complementary to segment 3. Virology 177:372–374
    [Google Scholar]
  9. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DN A sequencing. Gene 28:351–359
    [Google Scholar]
  10. Ishikawa K., Ohmura T., Tsuchizaki T. 1989; Association of double- and single-stranded RNAs with each of the four components of rice stripe virus. Annals of the Phytopathological Society of Japan 55:315–323
    [Google Scholar]
  11. Koganezawa H., Doi Y., Yora K. 1975; Purification of rice stripe virus. Armais of the Phytopathological Society of Japan 41:148–154
    [Google Scholar]
  12. Lehrach H., Diamond D., Wozney J. F., Boedtker H. 1977; RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
    [Google Scholar]
  13. Masaki T., Fujihashi T., Nakamura K., Soejima M. 1981; Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1: II. Specificity and inhibition studies of Achromobacter protease I. Biochimica et biophysica acta 660:51–55
    [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  15. Smith L. M., Senders J. Z., Kaiser R. J., Hughes P., Dodd C., Connell C. R., Heiner C., Kent S. B. H., Hood L. E. 1986; Fluorescence detection in automated DNA sequence analysis. Nature, London 321:674–679
    [Google Scholar]
  16. Takahashi M., Toriyama S., Kikuchi Y., Hayakawa T., Ishihama A. 1990; Complementarity between the 5′- and 3′-terminal sequences of rice stripe virus RNAs. Journal of General Virology 71:2817–2821
    [Google Scholar]
  17. Toriyama S. 1982; Three ribonucleic acids associated with rice stripe virus. Annals of the Phytopathological Society of Japan 48:482–489
    [Google Scholar]
  18. Toriyama S. 1986a; Rice stripe virus: prototype of a new group of viruses that replicate in plants and insects. Microbiological Sciences 3:347–351
    [Google Scholar]
  19. Toriyama S. 1986b; An RNA-dependent RNA polymerase associated with the filamentous nucleoproteins of rice stripe virus. Journal of General Virology 67:1247–1255
    [Google Scholar]
  20. Toriyama S., Watanabe Y. 1989; Characterization of single- and double-stranded RNAs in particles of rice stripe virus. Journal of General Virology 70:505–511
    [Google Scholar]
  21. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-4-763
Loading
/content/journal/jgv/10.1099/0022-1317-72-4-763
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error