1887

Abstract

Antigenic and genomic relationships between five pairs of non-cytopathic and cytopathic bovine viral diarrhoea (BVD) viruses isolated from cattle with mucosal disease were examined. Antigenic similarity was evaluated by studying the binding characteristics of 10 monoclonal antibodies (MAbs) directed against the gp53 BVD virus glycoprotein. The MAb binding match between members of the same virus pair ranged from 10/10 to 7/10. The genomic relationship was evaluated by studying the hybridization characteristics of two cDNA probes and six complementary 20 base oligomer probes with virus DNA, selected on the basis of conservation between published BVD virus sequences; cDNA probes were hybridized at two stringencies (60 °C and 45 °C). The match of hybridization results between members of the same virus pair ranged from 4/4 to 1/4 with the cDNA probes and from 6/6 to 3/6 with the oligomer probes. The results indicate that members of the same virus pair can differ at both the antigenic and genomic levels.

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1991-03-01
2024-12-03
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References

  1. Afshar A., Gilles C., Bouffard A. 1989; Application of peroxidase-labelled antibody assays for detection of porcine IgG antibodies to hog cholera and bovine viral diarrhea viruses. Journal of Virological Methods 23:253–262
    [Google Scholar]
  2. Beaucage S. L., Caruthers M. H. 1981; Deoxynucleoside phosphoramidites - a new class of key intermediates for deoxypolynucleotide synthesis. Tetrahedron Letters 22:1859–1862
    [Google Scholar]
  3. Bolin S. R., McClurkin A. W., Cutup R. C., Coria M. F. 1985a; Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. American Journal of Veterinary Research 46:573–576
    [Google Scholar]
  4. Bolin S. R., McClurkin A. W., Cutlip R. C., Coria M. F. 1985b; Response of cattle persistently infected with noncytopathic bovine viral diarrhea virus to vaccination of bovine viral diarrhea and to subsequent challenge exposure with cytopathic bovine viral diarrhea virus. American Journal of Veterinary Research 46:2467–2470
    [Google Scholar]
  5. Bolin S. R., Moennig V., Kelso Vourley N. E., Ridpath J. F. 1988; Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Archives of Virology 99:117–124
    [Google Scholar]
  6. Brownlie J., Clarke M. C., Howard C. J. 1984; Experimental production of fatal mucosal disease in cattle. Veterinary Records 114:535–536
    [Google Scholar]
  7. Collett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchio A. F. 1988; Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165:191–199
    [Google Scholar]
  8. Collett M. S., Moennig V., Horzinbk M. C. 1989; Recent advances in pestivirus research. Journal of General Virology 70:253266
    [Google Scholar]
  9. Corapi W. V., Donis R. O., Dubovi E. F. 1988; Monoclonal antibody analysis of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. Journal of Virology 62:2823–2827
    [Google Scholar]
  10. Gillespie J. H., Baker J. A., McEntee K. 1960; A cytopathogenic strain of virus diarrhea virus. Cornell Veterinarian 50:73–79
    [Google Scholar]
  11. Gillespie J. H., Madin S. H., Darby N. B. 1962; Cellular resistance in tissue culture, induced by noncytopathogenic strains, to a cytopathogenic strain of virus diarrhea virus of cattle. Proceedings of the Society for Experimental Biology and Medicine 110:248–250
    [Google Scholar]
  12. Howard C. J., Brownlie J., Clarke M. C. 1987; Comparison by the neutralization assay of pairs of non-cytopathogenic and cytopathogenic strains of bovine virus diarrhoea virus isolated from cases of mucosal disease. Veterinary Microbiology 13:361–369
    [Google Scholar]
  13. Koch J., Kovraa S., Bolund L. 1986; An improved method for labelling of DNA probes by nick translation. Nucleic Acids Research 14:7132
    [Google Scholar]
  14. Lee K. M., Gillespie J. H. 1957; Propagation of virus diarrhea virus of cattle in tissue culture. American Journal of Veterinary Research 18:953
    [Google Scholar]
  15. Renard A., Guiot C., Schmetz D., Dagenais L., Pastoret P.-P., Dina D., Martial J. A. 1985; Molecular cloning of bovine viral diarrhea viral sequences. DNA 4:429–438
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
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