RT Journal Article SR Electronic(1) A1 Endo, Atsushi A1 Itamura, Shigeyuki A1 Iinuma, Hisae A1 Funahashi, Shin-ichi A1 Shida, Hisatoshi A1 Koide, Fusaoki A1 Nerome, Kuniaki A1 Oya, AkiraYR 1991 T1 Homotypic and heterotypic protection against influenza virus infection in mice by recombinant vaccinia virus expressing the haemagglutinin or nucleoprotein gene of influenza virus JF Journal of General Virology, VO 72 IS 3 SP 699 OP 703 DO https://doi.org/10.1099/0022-1317-72-3-699 PB Microbiology Society, SN 1465-2099, AB Recombinant vaccinia virus expressing the influenza virus haemagglutinin (HA) or nucleoprotein (NP) genes from A/SW/Hong Kong/1/74 (H1N1) under the control of a hybrid promoter containing the P7.5 early promoter element and promoter of the gene encoding the major protein of cowpox virus A type inclusion body was constructed to investigate protective immunity against homologous and heterologous viruses in mice. These recombinant vaccinia viruses produced authentic influenza virus HA and NP in infected cells. The recombinant vaccinia virus-influenza virus HA conferred efficient subtype-specific protection although mice challenged with heterologous influenza viruses underwent initial infection. By contrast, immunization with the recombinant vaccinia-influenza virus NP limited virus multiplication in the lungs against challenge infection with all H1N1 and H3N2 influenza viruses examined, although less efficiently. These results will prompt the re-examination of the possibility of using the recombinant vaccinia virus-influenza virus NP as a cross-protective vaccine, UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-3-699