1887

Abstract

Recombinant vaccinia virus expressing the influenza virus haemagglutinin (HA) or nucleoprotein (NP) genes from A/SW/Hong Kong/1/74 (H1N1) under the control of a hybrid promoter containing the P7.5 early promoter element and promoter of the gene encoding the major protein of cowpox virus A type inclusion body was constructed to investigate protective immunity against homologous and heterologous viruses in mice. These recombinant vaccinia viruses produced authentic influenza virus HA and NP in infected cells. The recombinant vaccinia virus-influenza virus HA conferred efficient subtype-specific protection although mice challenged with heterologous influenza viruses underwent initial infection. By contrast, immunization with the recombinant vaccinia-influenza virus NP limited virus multiplication in the lungs against challenge infection with all H1N1 and H3N2 influenza viruses examined, although less efficiently. These results will prompt the re-examination of the possibility of using the recombinant vaccinia virus-influenza virus NP as a cross-protective vaccine

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1991-03-01
2023-01-28
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References

  1. Ada G. L., Jones P. D. 1986; The immune response to influenza infection. Current Topics in Microbiology and Immunology 128:1–54
    [Google Scholar]
  2. Ada G. L., Mak N. K., Sweet C. 1983; The regulation of influenza virus infection. Progress in Immunology 5:1295–1304
    [Google Scholar]
  3. Andrew M. E., Coupar B. E. H. 1988; Efficacy of influenza haemagglutinin and nucleoprotein as protective antigens against influenza virus infection in mice. Scandinavian Journal of Immunology 28:81–85
    [Google Scholar]
  4. Andrew M. E., Coupar B. E. H., Boyle D. B., Ada G. L. 1987; The roles of influenza virus haemagglutinin and nucleoprotein in protection: analysis using vaccinia virus recombinants. Scandinavian Journal of Immunology 25:21–28
    [Google Scholar]
  5. Askonas B. A., McMichael A. J., Webster R. G. 1982; The immune response to influenza viruses and the problem of protection against infection. In Basic and Applied Influenza Research pp 159–188 Edited by Beare A. S. Boca Raton: CRC Press;
    [Google Scholar]
  6. Bennink J. R., Yewdell J. W., Smith G. L., Moller C., Moss B. 1984; Recombinant vaccinia virus primes and stimulates influenza haemagglutinin-specific cytotoxic T cells. Nature, London 311:578–579
    [Google Scholar]
  7. Chambers T. M., Kawaoka Y., Webster R. G. 1988; Protection of chicken from lethal influenza infection by vaccinia-expressed hemagglutinin. Virology 167:414–421
    [Google Scholar]
  8. Coupar B. E. H., Andrew M. E., Both G. W., Boyle D. B. 1986; Temporal regulation of influenza haemagglutinin expression in vaccinia virus recombinants and effects on the immune response. European Journal of Immunology 16:1479–1487
    [Google Scholar]
  9. Davison A. J., Moss B. 1989; Structure of vaccinia virus late promoters. Journal of Molecular Biology 210:771–784
    [Google Scholar]
  10. Ennis F. A., Wells M. A., Butchko G. M., Albrecht P. 1978; Evidence that cytotoxic T cells are part of the host’s response to influenza pneumonia. Journal of Experimental Medicine 148:1241–1250
    [Google Scholar]
  11. Flexner C., Hugin A., Moss B. 1988; Attenuation of live recombinant vaccinia virus vectors by expression of human interleukin-2. In Vaccines 88 pp 179–184 Edited by Ginsberg H., Brown F., Lemer R. A., Chanock R. M. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Gotch F., McMichael A., Smith G. L., Moss B. 1987; Identification of viral molecules recognised by influenza-specific human cytotoxic T lymphocytes. Journal of Experimental Medicine 165:408–416
    [Google Scholar]
  13. Hunt L. A., Brown D. W., Robinson H. L., Naeve C. W., Webster R. G. 1988; Retrovirus-expressed hemagglutinin protects against lethal influenza virus infections. Journal of Virology 62:3014–3019
    [Google Scholar]
  14. Hurd J., Heath R. B. 1975; Effect of cyclophosphamide on infections in mice caused by virulent and avirulent strains of influenza virus. Infection and Immunity 11:886–889
    [Google Scholar]
  15. Itamura S., Morikawa Y., Shida H., Nerome K., Oya A. 1990a; Biological and immunological characterization of influenza virus haemagglutinin expressed from the haemagglutinin locus of vaccinia virus. Journal of General Virology 71:1293–1301
    [Google Scholar]
  16. Itamura S., Iinuma H., Shida H., Morikawa Y., Nerome K., Oya A. 1990b; Characterization of antibody and cytotoxic T lymphocyte responses to human influenza virus H3 haemagglutinin expressed from the haemagglutinin locus of vaccinia virus. Journal of General Virology 71:2859–2865
    [Google Scholar]
  17. McMichael A. J., Michie C. A., Gotch F. M., Smith G. L., Moss B. 1986; Recognition of influenza A virus nucleoprotein by human cytotoxic T lymphocytes. Journal of General Virology 67:719–726
    [Google Scholar]
  18. Panicali D., Davis S. W., Weinberg R. L., Paoletti E. 1983; Construction of live vaccines by using genetically engineered poxviruses: biological activity of recombinant vaccinia virus expressing influenza virus hemagglutinin. Proceedings of the National Academy of Sciences, U.S.A. 80:5364–5368
    [Google Scholar]
  19. Singer S. H., Noguchi P., Kirschstein R. L. 1972; Respiratory diseases in cyclophosphamide-treated mice. II. Decreased virulence of PR8 influenza virus. Infection and Immunity 5:957–960
    [Google Scholar]
  20. Smith G. L., Murphy B. R., Moss B. 1983; Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters. Proceedings of the National Academy of Sciences, U.S.A 80:7155–7159
    [Google Scholar]
  21. Stitz L., Schmitz C., Binder D., Zinkernagel R., Paoletti E., Becht H. 1990; Characterization and immunological properties of influenza A virus nucleoprotein (NP): cell-associated NP isolated from infected cells or viral NP expressed by vaccinia recombinant virus do not confer protection. Journal of General Virology 71:1169–1179
    [Google Scholar]
  22. Sugita S., Yoshioka Y., Itamura S., Kanegae Y., Oguchi K., Gojobori T., Nerome K., Oya A. 1991; Molecular evolution of hemagglutinin genes of H1N1 swine and human influenza A viruses. Journal of Molecular Evolution (in press)
    [Google Scholar]
  23. Sullivan J. L., Mayner R. E., Barry D. W., Ennis F. A. 1976; Influenza virus infection in nude mice. Journal of Infectious Diseases 133:91–94
    [Google Scholar]
  24. Taylor P. M., Askonas B. A. 1986; Influenza nucleoprotein-specific cytotoxic T-cell clones are protective in vivo . Immunology 58:417–420
    [Google Scholar]
  25. Taylor J., Weinberg R., Kawaoka Y., Webster R. G., Paoletti E. 1988; Protective immunity against avian influenza induced by a fowlpox virus recombinant. Vaccine 6:504–508
    [Google Scholar]
  26. Tobita K., Sugiura A., Enomoto C., Furuyama M. 1975; Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Medical Microbiology and Immunology 162:9–14
    [Google Scholar]
  27. Townsend A., Bastin J., Gould K., Brownlee G., Andrew M., Coupar B., Boyle D., Chan S., Smith G. 1988; Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen. Journal of Experimental Medicine 168:1211–1224
    [Google Scholar]
  28. Virelizier J. L., Allison A. C., Schild G. C. 1979; Immune responses to influenza virus in the mouse and their role in control of infection. British Medical Bulletin 35:65–68
    [Google Scholar]
  29. Yewdell J. W., Bennink J. R., Smith G. L., Moss B. 1985; Influenza A virus nucleoprotein is a major target antigen for crossreactive anti-influenza A virus cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences, U.S.A 82:1785–1789
    [Google Scholar]
  30. Yilma T., Anderson K., Brechling K., Moss B. 1987; Expression of an adjuvant gene (interferon-γ) in infectious vaccinia virus recombinants. In Vaccines 87 pp 393–396 Edited by Chanock R. M., Lemer R. A., Brown F., Ginsberg H. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
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