1887

Abstract

Infection of MA-104 cells with the OSU strain of rotavirus induced an increase in Na and a decrease in K intracellular concentrations, starting at 4 h post-infection. These changes were not related to an inhibition of the Na/K pump since ouabain-sensitive Rb uptake was augmented in rotavirus-infected cells compared to control cells, whereas the [H]ouabain binding and Na/K ATPase activity in the cell homogenate were unaffected. Furosemide-sensitive Rb uptake (Na/K/2Cl cotransport) was not modified by the infection. Passive Rb efflux and Na influx were augmented in infected cells suggesting an increase in the plasma membrane permeability. The increase in intracellular Na concentration might be responsible for the observed stimulation of the Na/K pump. This effect was dependent upon the synthesis of viral proteins because it was abolished by addition of cycloheximide up to 4 h post-infection. Prevention of the increase in intracellular Na by the use of low Na-containing media did not modify the pattern of protein synthesis. This suggests that changes in intracellular Na and K concentrations were not related to shutoff of cellular protein synthesis. Alterations of ion contents in the rotavirus-infected enterocytes might impair intestinal absorptive capacity before the appearance of histopathological lesions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-3-541
1991-03-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/3/JV0720030541.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-3-541&mimeType=html&fmt=ahah

References

  1. Carpio M. M., Babiuk L. A., Misra V., Blumenthal R. M. 1981; Bovine rotavirus-cell interactions: effect of virus infection on cellular integrity and macromolecular synthesis. Virology 114:86–97
    [Google Scholar]
  2. Carrasco L., Lacal J. C. 1983; Permeabilization of cells during animal virus infection. Pharmacology and Therapeutics 23:109–145
    [Google Scholar]
  3. Crouch C. F., Woode G. N. 1978; Serial studies of virus multiplication and intestinal damage in gnotobiotic piglets infected with rotavirus. Journal of Medical Microbiology 11:325–333
    [Google Scholar]
  4. Del Castillo J. R., Robinson J. W. L. 1985; A ouabain-insensitive, Na+-stimulated ATPase in basolateral plasma membrane of guinea pig small intestinal cells. Biochimica et biophysica acta 812:413–422
    [Google Scholar]
  5. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  6. Forbush B. III 1983; Assay of Na, K-ATPase in plasma membrane preparations: increasing the permeability of membrane vesicles using sodium dodecyl sulphate buffered with bovine serum albumin. Analytical Biochemistry 128:159–163
    [Google Scholar]
  7. Francoeur A. M., Stanners C. P. 1978; Evidence against the role of K+ in the shut-off of protein synthesis by vesicular stomatitis virus. Journal of General Virology 39:551–554
    [Google Scholar]
  8. Frugulhetti I. C. P. P., Rebello M. A. 1989; Na+ and K+ concentration and regulation of protein synthesis in L-A9 and Aedes albopictus cells infected with marituba virus (Bunyaviridae). Journal of General Virology 70:3493–3499
    [Google Scholar]
  9. Gadd K. G. 1981; Protein estimation in spinal fluid using Coomassie blue reagent. Medical Laboratory Science 38:61–63
    [Google Scholar]
  10. Garry R. F., Bishop J. M., Parker S., Westbrook K., Lewis G., Waite M. R. F. 1979; Na+ and K+ concentrations and the regulation of protein synthesis in Sindbis virus-infected chick cells. Virology 96:108–120
    [Google Scholar]
  11. Gerna G., Battaglia M., Milanesi G., Passarani N., Perci-Valle E., Cattaneo E. 1984; Serotyping of cell-culture adapted subgroup 2 human rotavirus strains by neutralization. Infection and Immunity 43:722–729
    [Google Scholar]
  12. Gorziglia M., Larrea C., Liprandi F., Esparza J. 1985; Biochemical evidence for the oligomeric (possibly trimeric) structure of the major inner capsid polypeptide (45K) of rotaviruses. Journal of General Virology 66:1889–1900
    [Google Scholar]
  13. Hackstadt T., Mallavia L. P. 1982; Sodium and potassium transport in herpes simplex virus-infected cells. Journal of General Virology 60:199–207
    [Google Scholar]
  14. Kaljot K. T., Shaw D. H., Rubin D. H., Greenberg H. B. 1988; Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis. Journal of Virology 62:1136–1144
    [Google Scholar]
  15. Kletzien R. F., Pariza M. W., Becker J. E., Potter V. R. 1975; A method using 3-O-methyl-d-glucose and phloretin for the determination of intracellular water space of cells in monolayer culture. Analytical Biochemistry 68:537–544
    [Google Scholar]
  16. Lacal J. C., Carrasco L. 1982; Relationship between membrane integrity and the inhibition of host translation in virus-infected mammalian cells. Comparative studies between encephalomyocarditis virus and poliovirus. European Journal of Biochemistry 127:359–366
    [Google Scholar]
  17. Lopez-Rivas A., Castrillo J. L., Carrasco L. 1987; Cation content in poliovirus-infected HeLa cells. Journal of General Virology 68:335–342
    [Google Scholar]
  18. Michelangeli F., Ruiz M. C., del Castillo J. R., Ludert J. E., Liprandi F. 1991; Effect of rotavirus infection on intracellular calcium homeostasis in cultured cells. Virology 181: (in press)
    [Google Scholar]
  19. Mizzen L., Macintyre G., Wong F., Anderson R. 1987; Translational regulation in mouse hepatitis virus infection is not mediated by altered intracellular ion concentrations. Journal of General Virology 68:2143–2151
    [Google Scholar]
  20. Nair C. N. 1984; Na and K changes in animal virus-infected HeLa cells. Journal of General Virology 65:1135–1138
    [Google Scholar]
  21. Nokta M., Fons M. P., Eaton D. C., Albrecht T. 1988; Cytomegalovirus: sodium entry and development of cytomegaly in human fibroblasts. Virology 164:411–419
    [Google Scholar]
  22. Rey O., Rossi J. P. F. C., Lopez R., Iapalucci-Espinoza S. J., Franze-Fernandez M. T. 1988; Tacaribe virus infection may induce inhibition of the activity of the host cell Ca2+ and Na+/K+ pumps. Journal of General Virology 69:951–954
    [Google Scholar]
  23. Theil K. W., Bohl E. H., Cross R. F., Kohler E. M., Agnes A. G. 1978; Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. American Journal of Veterinary Research 39:213–220
    [Google Scholar]
  24. Ulug E. T., Garry R. F., Waite M. R. F., Bose H. R. 1984; Alterations in monovalent cation transport in Sindbis virus-infected chick cells. Virology 132:118–130
    [Google Scholar]
  25. Wagner R. K. 1984; Cytopathic effect of viruses: a general survey. In Comprehensive Virology vol 19 pp 1–63 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-3-541
Loading
/content/journal/jgv/10.1099/0022-1317-72-3-541
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error