@article{mbs:/content/journal/jgv/10.1099/0022-1317-72-3-505, author = "Burgyan, Jòzsef and Rubino, Luisa and Russo, Marcello", title = "De novo generation of cymbidium ringspot virus defective interfering RNA", journal= "Journal of General Virology", year = "1991", volume = "72", number = "3", pages = "505-509", doi = "https://doi.org/10.1099/0022-1317-72-3-505", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-3-505", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = " Nicotiana clevelandii plants were inoculated with cymbidium ringspot tombusvirus RNA synthesized in vitro, after which further passages were made by sap inoculation. During the third passage, low M r RNA species appeared which had the characteristics of deletion mutants of genomic RNA. Sequence analysis of several of these defective interfering RNAs suggested a possible evolution of smaller from larger molecules. Computer-generated secondary structures of sequences surrounding recombination sites were extensive and stable and these sites occurred in interior or hairpin loops, thus providing a possible explanation for discontinuous RNA transcription and the formation of deletions in genomic RNA.", }