1887

Abstract

The 3′-terminal region of wheat streak mosaic virus (WSMV) genomic RNA was cloned and a cDNA sequence of 1809 nucleotides upstream of the poly(A) tract was determined. The sequence contains a single open reading frame of 1662 nucleotides and a 3′ untranslated region of 147 nucleotides. Translation products from WSMV RNA and WSMV cDNA transcripts were immunoprecipitated by WSMV capsid protein antiserum, indicating that the 3′-terminal region of WSMV RNA encodes the capsid protein. Five potential N-terminal capsid protein protease cleavage sites were identified, which would yield proteins ranging from 31.7K to 46.8K. Alignment of the deduced amino acid sequence of the WSMV capsid protein with those of other potyviruses showed significant, but limited, identity as compared to the alignment of two or more aphid-transmitted potyviruses. Although WSMV has characteristics distinct from poty-viruses, because of its particle morphology, translation strategy apparently based on polyprotein processing, the ability to form cytoplasmic cylindrical inclusions and the degree of capsid protein homology with aphidtransmitted potyviruses, it should be considered a member of the potyvirus group.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-3-499
1991-03-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/3/JV0720030499.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-3-499&mimeType=html&fmt=ahah

References

  1. Aebersold R. H., Leavitt J., Saaverda R. A., Hood L. E., Kent S. B. H. 1987; Internal amino acid analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A. 84:6970–6974
    [Google Scholar]
  2. Allison R., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  3. Atreya C. D., Raccah B., Pirone T. P. 1990; A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178:161–165
    [Google Scholar]
  4. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  5. Brakke M. K. 1971; Wheat streak mosaic virus. CMI/AAB Descriptions of Plant Viruses no. 48
    [Google Scholar]
  6. Brakke M. K., Ball E. M., Hsu Y. H., Langenberg W. G. 1987; Wheat streak mosaic virus cylindrical inclusion body protein. Journal of General Virology 68:281–287
    [Google Scholar]
  7. Carrington J. C., Dougherty W. G. 1988; A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proceedings of the National Academy of Sciences, U.S.A 85:3391–3395
    [Google Scholar]
  8. Domier L. L., Franklin K. M., Shahabuddin M., Hellman G., Overmeyer J. H., Hiremath S. T., Siaw M. F. E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5429
    [Google Scholar]
  9. Dougherty W. G., Hiebert E. 1980a; Translation of potyvirus RNA in a rabbit reticulocyte lysate: reaction conditions and identification of capsid protein as one of the products of in vitro translation of tobacco etch and pepper mottle viral RNAs. Virology 101:466–474
    [Google Scholar]
  10. Dougherty W. F., Hiebert E. 1980b; Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins as products of tobacco etch virus RNA translations and cylindrical inclusion protein as a product of the potyvirus genome. Virology 104:174–182
    [Google Scholar]
  11. Gough K. H., Azad A. A., Hanna P. J., Shukla D. D. 1987; Nucleotide sequence of the capsid and nuclear inclusion protein genes from the Johnson grass strain of sugarcane mosaic virus RNA. Journal of General Virology 68:297–304
    [Google Scholar]
  12. Grumet R., Fang G. 1990; cDNA cloning and sequence analysis of the 3′-terminal region of zucchini yellow mosaic virus RNA. Journal of General Virology 71:1619–1622
    [Google Scholar]
  13. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  14. Hiebert E., Thornbury D. W., Pirone T. P. 1984; Immunoprecipitation analysis of potyviral in vitro translation products using antisera to helper component of tobacco vein mottling virus and potato virus Y. Virology 135:1–9
    [Google Scholar]
  15. Hollings M., Brunt A. A. 1981; Potyvirus group. CMI/AAB Descriptions of Plant Viruses no. 245
    [Google Scholar]
  16. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  17. Hunkapiller M. W., Hewick R. M., Dreyer R. J., Hood L. E. 1983; High sensitivity sequencing with a gas-phase sequencer. Methods in Enzymology 91:399–413
    [Google Scholar]
  18. Kamer G., Argos P. 1984; Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  19. Kashiwazaki S., Hayano Y., Minobe Y., Omura T., Hibino H., Tsuchizaki T. 1989; Nucleotide sequence of the capsid protein gene of barley yellow mosaic virus. Journal of General Virology 70:3015–3023
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature, London 227:680685
    [Google Scholar]
  21. Lane L. C., Skopp R. 1983; The coat protein of wheat streak mosaic virus. Phytopathology 73:791
    [Google Scholar]
  22. Lommel S. A., McCain A. H., Morris T. J. 1982; Evaluation of indirect enzyme-linked immunosorbent assay for the detection of plant viruses. Phytopathology 72:1018–1022
    [Google Scholar]
  23. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. W. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  24. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Matsudaira P. 1987; Sequence from picomolar quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry 261:10035–10038
    [Google Scholar]
  26. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  27. Morris T. J., Hess R. T., Pinnock D. E. 1979; Physicochemical characterization of a small RNA virus associated with baculovirus infection in Trichoplusia ni . Intervirology 11:238–247
    [Google Scholar]
  28. Nakazato H., Edmonds M. 1974; Purification of messenger RNA and heterogeneous nuclear RNA containing poly(A) sequences. Methods in Enzymology 29:431–443
    [Google Scholar]
  29. Pfannenstiel M. A., Niblett C. L. 1978; The nature of the resistance of agrotricums to wheat streak mosaic virus. Phytopathology 68:1204–1209
    [Google Scholar]
  30. Rhoads D. D., Roufa D. S. 1985; Emetine resistance of Chinese hamster cells: structure of wild type and mutant ribosomal protein S14 messenger RNA species. Molecular and Cellular Biology 5:1655–1659
    [Google Scholar]
  31. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  33. Shukla D. D., Ward C. W. 1988; Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  34. Shukla D. D., Ford R. E., Tosic M., Jilka J., Ward C. W. 1989; Possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group. Archives of Virology 105:143–151
    [Google Scholar]
  35. Sim T. IV, Willis W. G., Eversmeyer M. G. 1988; Kansas plant disease survey. Plant Disease 72:832–836
    [Google Scholar]
  36. Slykhuis T. T. 1953; Wheat streak mosaic virus in Alberta and factors related to its spread. Canadian Journal of Agricultural Science 33:195–197
    [Google Scholar]
  37. Stoddard S. L., Lommel S. A., Gill B. S. 1987; Evaluation of wheat germ plasm for resistance to wheat streak mosaic virus by symptomatology, ELISA, and slot-blot hybridization. Plant Disease 71:714–719
    [Google Scholar]
  38. Zagula K. R., Kendall T. L., Lommel S. A. 1990; Wheat streak mosaic virus genomic RNA shares sequence homology with potyviral cylindrical inclusion cistrons. Phytopathology 80:10–36
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-72-3-499
Loading
/content/journal/jgv/10.1099/0022-1317-72-3-499
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error