1887

Abstract

Vaccinia-measles recombinant viruses were used to examine the contribution of the individual measles virus glycoproteins in fusion. Although vaccinia virus recombinants expressing either the haemagglutinin or fusion proteins did not induce fusion in the cell lines examined, a double recombinant expressing both measles virus glycoproteins gave extensive syncytia in cells of human and simian origin. No fusion was observed in mouse, hamster or chicken cells. The fusion induced by the double recombinant could be specifically inhibited with either anti-fusion or anti-haemagglutinin monoclonal antibodies.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-2-439
1991-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/2/JV0720020439.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-2-439&mimeType=html&fmt=ahah

References

  1. Alkhatib G., Richardson C., Shen S.-H. 1990; Intracellular processing, glycosylation, and cell-surface expression of the measles virus fusion protein (F) encoded by a recombinant adenovirus. Virology 175:262–270
    [Google Scholar]
  2. Ashorn P. A., Berger E. A., Moss B. 1990; Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells. Journal of Virology 64:2149–2156
    [Google Scholar]
  3. Dalgleish A. G., Beverley P. C., Clapham P. R., Crawford D. H., Greaves M. F., Weiss R. A. 1984; The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature, London 312:763–766
    [Google Scholar]
  4. Drillien R., Spehner D., Kirn A., Giraudon P., Buckland R., Wild F., Lecocq J. P. 1988; Protection of mice from fatal encephalitis by vaccination with vaccinia virus recombinants encoding either the hemagglutinin or the fusion protein. Proceedings of the National Academy of Sciences, U.S.A 85:1252–1256
    [Google Scholar]
  5. Klatzmann D., Champagne E., Chamaret S., Gruest J., Guetard D., Hercend T., Gluckman J. C., Montagnier L. 1984; T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV. Nature, London 312:767–768
    [Google Scholar]
  6. McClure M. O., Marsh M., Weiss R. A. 1987; Human immunodeficiency virus infection of CD4-bearing cells by a pH-independent mechanism. EMBO Journal 7:513–518
    [Google Scholar]
  7. Malvoisin E., Wild F. 1990a; Effect of drugs which inhibit cholesterol synthesis on syncytia formation in Vero cells infected with measles virus. Biochimica et biophysica acta 1042:359–364
    [Google Scholar]
  8. Malvoisin E., Wild T. F. 1990b; Contribution of measles virus fusion protein in protective immunity: anti-F monoclonal antibodies neutralize virus infectivity and protect mice against challenge. Journal of Virology 64:5160–5162
    [Google Scholar]
  9. Marsh M., Helenius A. 1988; Virus entry into animal cells. Advances in Virus Research 36:107–149
    [Google Scholar]
  10. Norrby E. 1971; The effect of a carbobenzoxy tripeptide on the biological activities of measles virus. Virology 44:599–608
    [Google Scholar]
  11. Norrby E., Gollmar Y. 1972; Appearance and persistence of antibodies against different virus components after regular measles infection. Infection and Immunity 6:240–247
    [Google Scholar]
  12. Richardson C. D., Scheid A., Choppin P. W. 1980; Specific inhibition of paramyxovirus and myxovirus replication by oligopeptides with amino acid sequences similar to those at the N-termini of the F1 or HA2 viral polypeptides. Virology 105:205–222
    [Google Scholar]
  13. Ross D. S., Duchola C. S., Stephensen C. B., Holmes K. V., Choppin P. W. 1990; Control of virus-induced cell fusion by host cell lipid composition. Virology 175:345–357
    [Google Scholar]
  14. Sakai Y., Shibuta H. 1989; Syncytia formation by recombinant vaccinia viruses carrying bovine parainfluenza 3 virus envelope protein genes. Journal of Virology 63:3661–3668
    [Google Scholar]
  15. Stein B. S., Gowda S. D., Litson J. D., Penhallow R. C., Bensch K. G., Engleman E. G. 1987; pH-independent HIV entry into CD4-positive cells via virus envelope fusion to the plasma membrane. Cell 49:659–668
    [Google Scholar]
  16. Tersmette M., Van Dongen J. J. M., Clapham P. R., De Goede R. E. Y., Wolvers-Tettero I. L. M., Van Kessel A. G., Huisman J. G., Weiss R. A., Miedema F. 1989; Human immunodeficiency virus infection studied in CD4-expressing human-murine T-cell hybrids. Virology 168:267–273
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-2-439
Loading
/content/journal/jgv/10.1099/0022-1317-72-2-439
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error