Stabilization of human rhinovirus serotype 2 against pH-induced conformational change by antiviral compounds Free

Abstract

Four WIN compounds with anti-picornavirus activities were tested for their ability to stabilize human rhinovirus serotype 2 (HRV-2) against low pH-induced conformational changes , as determined by specific immunoprecipitation. These results were compared to the minimal inhibitory concentration (MIC) as measured in a plaque reduction assay. A direct relationship was observed between the concentration of the compound that prevented the low pH-induced conformational changes and the MIC, indicating that stabilization is an important element in the mode of action of these drugs against HRV-2.

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1991-02-01
2024-03-28
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References

  1. Davoust J., Gruenberg J., Howell K. E. 1987; The threshold values of low pH block endocytosis at different stages. EMBO Journal 6:3601–3609
    [Google Scholar]
  2. Helenius A., Kartenbeck J., Simons K., Fries E. 1980; On the entry of Semliki Forest virus into BHK-21 cells. Journal of Cell Biology 84:404–420
    [Google Scholar]
  3. Kim S., Smith T. J., Chapman M. S., Rossmann M. G., Pevear D.C., Dutko F. J., Felock P. J., Diana G. D., name McKinlay M. A. 1989; Crystal structure of human rhinovirus serotype 1A (HRV1A). Journal of Molecular Biology 210:91–111
    [Google Scholar]
  4. Korant B. D., Lonberg-Holm K., Noble J., Stasny J. T. 1972; Naturally occurring and artificially produced components of three rhinoviruses. Virology 48:71–86
    [Google Scholar]
  5. Kowalski H., Maurer-Fogy I., Vriend G., Casari G., Beyer A., Blaas D. 1989; Trypsin sensitivity of several human rhinovirus serotypes in their low pH induced conformation. Virology 171:611–614
    [Google Scholar]
  6. Lonberg-Holm K., Whiteley N. M. 1976; Physical requirements for early interaction of poliovirus and human rhinovirus with HeLa cells. Journal of Virology 19:857–870
    [Google Scholar]
  7. Madshus I. G., Olsnes S., Sandvig K. 1984a; Different pH requirements for entry of two picornaviruses, human rhinovirus 2 and murine encephalomyocarditis virus. Virology 139:346–357
    [Google Scholar]
  8. Madshus I. H., Olsnes S., Sandvig K. 1984b; Requirements for entry of poliovirus RNA into cells at low pH. EMBO Journal 3:1945–1950
    [Google Scholar]
  9. Neubauer CH., Frasel L., Kuechler E., Blaas D. 1987; Mechanism of entry of human rhinovirus into HeLa cells. Virology 158:255–258
    [Google Scholar]
  10. Pevear D. C., Fancher M. J., Felock P. J., Rossmann M. G., Miller M. S., Diana G., Treasurywala A. M., McKinlay M. A., Dutko F. J. 1989; Conformational change in the floor of the human rhinovirus canyon blocks adsorption to HeLa cell receptors. Journal of Virology 63:2002–2007
    [Google Scholar]
  11. Rossmann M. G. 1989; Neutralization of small RNA viruses by antibodies and antiviral agents. FASEB Journal 3:2335–2343
    [Google Scholar]
  12. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H.-J., Johnson J. E., Kamer G., Luo M., Mosser A. M., Rueckert R. R., Sherry B. A., Vriend G. 1985; Structure of a common cold virus, human rhinovirus 14. Nature, London 317:145–154
    [Google Scholar]
  13. Smith T. J., Kremer J., Luo M., Vriend G., Arnold E., Kamer G., Rossmann M. G., McKinlay M. A., Diana G. D., Otto M. J. 1986; The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating. Science 233:1286–1293
    [Google Scholar]
  14. Woods M. G., Diana G. D., Rogge M. C., Otto M. J., Dutko F. J., McKinlay M. A. 1989; In vitro and in vivo activities of WIN54954, a new broad-spectrum anti-picornavirus drug. Antimicrobial Agents and Chemotherapy 33:2069–2074
    [Google Scholar]
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