RT Journal Article SR Electronic(1) A1 Misko, Ihor S. A1 Schmidt, Christopher A1 Moss, Denis J. A1 Burrows, Scott R. A1 Sculley, Thomas B.YR 1991 T1 Cytotoxic T lymphocyte discrimination between type A Epstein—Barr virus transformants is mapped to an immunodominant epitope in EBNA 3 JF Journal of General Virology, VO 72 IS 2 SP 405 OP 409 DO https://doi.org/10.1099/0022-1317-72-2-405 PB Microbiology Society, SN 1465-2099, AB An immunodominant Epstein—Barr virus (EBV)-specific cytotoxic T lymphocyte (CTL) epitope, represented by peptide 68, has been mapped to the EBV nuclear antigen, EBNA 3. The epitope is recognized by class I-restricted CTLs through HLA-B8 and is functionally active on type A but not type B lymphoblastoid cell lines (LCLs). Herein we show that peptide 68 is not expressed as a functional CTL epitope by type A LCLs infected with an EBV B95-8 isolate. CTLs from cultures stimulated with autologous type A IARC-BL74 or QIMR-WIL LCLs lysed autologous cells stimulated with phytohaemagglutinin (PHA blasts) and coated with exogenous peptide 68. No peptide 68-specific CTLs were generated in cultures stimulated with autologous type A B95-8 or type B AG876 LCLs. However, the B95-8 LCL coated with peptide 68 was effective in the induction of a peptide-specific CTL response. A peptide 68-specific CTL clone failed to lyse the B95-8 LCL, type B AG876 LCL and PHA blasts, although such targets were lysed when coated with peptide 68., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-2-405