1887

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Volume 72, Issue 2

Characterization of rotavirus guanylyltransferase activity associated with polypeptide VP3 Free

Abstract

Rotaviruses transcribe mRNA containing a 7GpppGp cap at the 5′ end . Guanylyltransferase activity associated with the viral particle was detected by SDS-PAGE due to the formation of a nucleotide-enzyme complex when the virus was incubated with [α-P]GTP. Using purified viral particles it was shown that only the core polypeptide VP3 exhibits the ability to form a complex with the nucleotide. The reaction is specific for GTP or dGTP when Mg is used as a cofactor. The reaction also depends on the incubation temperature and the pH, as described for other guanylyltransferases. The GMP-VP3 complex transfers the GMP to pyrophosphate, synthesizing GTP or GDP, resulting in the formation of a GpppG cap. These properties of the complex allowed the core polypeptide VP3 to be identified as the rotavirus guanylyltransferase.

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  • Accepted:
  • Published Online:
© Journal of General Virology 1991
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1991-02-01
2024-04-18
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Characterization of rotavirus guanylyltransferase activity associated with polypeptide VP3
J. Gen. Virol. 72, 325 (1991); https://doi.org/10.1099/0022-1317-72-2-325
/content/journal/jgv/10.1099/0022-1317-72-2-325
/content/journal/jgv/10.1099/0022-1317-72-2-325
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